2 documents found, page 1 of 1
Interaction of Hoechst 33342 with POPC Membranes at Different pH Values
Cordeiro, Margarida M.; Filipe, Hugo A. L.; Santos, Patrícia dos; Samelo, Jaime Amadeu Cruz Reis; Ramalho, João P Prates; Loura, Luís M. S.Hoechst 33342 (H33342) is a fluorescent probe that is commonly used to stain the DNA of living cells. To do so, it needs to interact with and permeate through cell membranes, despite its high overall charge at physiological pH values. In this work, we address the effect of pH in the association of H33342 with lipid bilayers using a combined experimental and computational approach. The partition of H33342 to 1-p...
Calculation of Permeability Coefficients from Solute Equilibration Dynamics: An...
Cordeiro, Margarida M.; Salvador, Armindo; Moreno, Maria JoãoPredicting the rate at which substances permeate membrane barriers in vivo is crucial for drug development. Permeability coefficients obtained from in vitro studies are valuable for this goal. These are normally determined by following the dynamics of solute equilibration between two membrane-separated compartments. However, the correct calculation of permeability coefficients from such data is not always strai...