49 documents found, page 1 of 5

Surtos de mpox em Portugal: resposta laboratorial e desafios no controlo da epi...

A infeção mpox, causada pelo vírus monkeypox (MPXV), tornou-se uma emergência global de saúde pública em 2022. Portugal foi um dos primeiros países a confirmar casos de infeção, o que levou à implementação de um sistema de vigilância laboratorial a nível nacional. Este estudo descreve a evolução epidemiológica de mpox em Portugal entre 2022 e 2025 e avalia o desempenho de diferentes tipos de amostras biológicas...

Mpox in People Living with HIV: Clinical Challenges, Preventive Strategies and ...

Monkeypox virus (MPXV) re-emerged in 2022 with a global outbreak that affected more than 100,000 individuals worldwide. People living with HIV (PLWH) accounted for a substantial proportion of cases, raising concerns about disease presentation, management, and outcomes in this population. Evidence indicates that PLWH with advanced or uncontrolled HIV infection experienced more severe mpox, with higher hospitaliz...

European pilot interlaboratory comparison study on Mpox virus whole genome sequ...

Objectives: Since 2022, distinct Mpox virus (MPXV) clades have been spreading across different geographic regions, causing a challenging epidemiological situation. Whole genome sequencing (WGS) proved to be instrumental for patient management and global public health. We report a pilot interlaboratory comparison study for MPXV WGS. Methods: We distributed noninfectious DNA samples, including the main MPXV clade...

Mpox in People Living with HIV: Clinical Challenges, Preventive Strategies and ...

Monkeypox virus (MPXV) re-emerged in 2022 with a global outbreak that affected more than 100,000 individuals worldwide. People living with HIV (PLWH) accounted for a substantial proportion of cases, raising concerns about disease presentation, management, and outcomes in this population. Evidence indicates that PLWH with advanced or uncontrolled HIV infection experienced more severe mpox, with higher hospitaliz...

The impact of orthopoxvirus vaccination and Mpox infection on cross-protective ...

Background: Cross-reactive immune memory responses to orthopoxviruses in humans remain poorly characterised despite their relevance for vaccine design and outbreak control. We aimed to assess the magnitude, specificity, and durability of cross-reactive immune responses elicited by smallpox vaccines and mpox virus infection. Methods: We did a multicohort observational study involving participants from the USA, B...

Undetected circulation of monkeypox virus in Portugal: Evidence for a 50-day ga...

As mpox continues to spread globally, proactive monitoring and preparedness are crucial to minimize impact and enhance response strategies. Using a mathematical model combining a negative binomial distribution with Richards' logistic curve, we reconstructed the hidden phase of mpox transmission in Portugal, offering insights into the timing and dynamics of the initial outbreak. The analysis of 950 PCR-positive ...

Mpox Surveillance and Laboratory Response in Portugal: Lessons Learned from Thr...

Background/Objectives: Mpox re-emerged in 2022 as a global health concern. Between 2022 and 2025, Portugal experienced three distinct outbreak waves, highlighting the critical role of laboratory surveillance and public health interventions. This study describes the epidemiological trends, diagnostic performance, and key lessons learned to improve outbreak preparedness. Methods: A total of 5610 clinical samples ...

Coordinated implementation of a conventional PCR assay to detect all Ebola and ...

Background: Filoviruses, including Ebola and Marburg viruses, cause severe hemorrhagic fever in humans and primates. These viruses pose significant threats to public health, making rapid and sensitive detection critical for controlling outbreaks. We developed and validated a hemi-nested generic PanFilo assay to detect all Ebola virus species, Marburg viruses, and recently discovered bat filoviruses. This assay ...

Probe-based metagenomic pathogen detection: advancing laboratory capacity for c...

Probe-based pathogen enrichment, followed by NGS, is a promising tool for complex diagnosis, overcoming traditional challenges of shotgun metagenomics, namely small microbial/human genetic material ratio and demanding computational resources. Here, we assessed the combined detection performance of two Illumina probe-based panels, the Respiratory and the Urinary Pathogen ID panels (RPIP and UPIP), using 99 clini...

Viral genetics and transmission dynamics in the second wave of mpox outbreak in...