9 documents found, page 1 of 1
Immunodiagnostic plasma amino acid residue biomarkers detect cancer early and p...
Tang, Cong; Corredeira, Patrícia; Casimiro, Sandra; Shi, Qi; Han, Qiwei; Sukdao, Wesley; Cavaco, Ana; Melo-Alvim, Cecília; Matos, Carolina OchôaThe immune response to tumour development is frequently targeted with therapeutics but remains largely unexplored in diagnostics, despite being stronger for early-stage tumours. We present an immunodiagnostic platform to detect this. We identify a panel of amino acid residue biomarkers providing a signature of cancer-specific immune activation associated with tumour development and distinct from autoimmune and ...
A randomized controlled trial assessing the release of circulating tumor and me...
Leitão, Tito Palmela; Corredeira, Patrícia; Rodrigues, Carolina; Piairo, Paulina; Miranda, Miguel; Martins Cavaco, Ana Cláudia; Kucharczak, SandraAbstract: Background: Circulating tumor cells (CTCs) may be the missing renal cell carcinoma (RCC) biomarker. No-touch (NT) resection has shown benefit in several tumors. Methods: A randomized controlled trial comparing CTC and circulating mesenchymal cell (CMC) release in no-touch (NT) vs. conventional (C) laparoscopic RN. Blood samples were collected at operation room arrival (S0), specimen extraction (S1), p...
Clinical validation of a size-based microfluidic device for circulating tumor c...
Palmela Leitão, Tito; Corredeira, Patrícia; Kucharczak, Sandra; Rodrigues, Margarida; Piairo, Paulina; Rodrigues, Carolina; Alves, PatríciaRenal cell carcinoma (RCC) presents as metastatic disease in one third of cases. Research on circulating tumor cells (CTCs) and liquid biopsies is improving the understanding of RCC biology and metastases formation. However, a standardized, sensitive, specific, and cost-effective CTC detection technique is lacking. The use of platforms solely relying on epithelial markers is inappropriate in RCC due to the freq...
MicroRNA‐181a restricts human γδ T cell differentiation by targeting Map3k2 and...
Gordino, Gisela; Costa‐Pereira, Sara; Corredeira, Patrícia; Alves, Patrícia; Costa, Luís; Gomes, Anita Q.; Silva‐Santos, Bruno; Ribot, Julie C.γδ T cells are a conserved population of lymphocytes that contributes to anti-tumor responses through its overt type 1 inflammatory and cytotoxic properties. We have previously shown that human γδ T cells acquire this profile upon stimulation with IL-2 or IL-15, in a differentiation process dependent on MAPK/ERK signaling. Here, we identify microRNA-181a as a key modulator of human γδ T cell differentiation. We...
Comprehensive genomic profiling of cell-free circulating tumor DNA detects resp...
Silveira, Catarina; Sousa, Ana Carla; Corredeira, Patrícia; Martins, Marta; Sousa, Ana Rita; Da Cruz Paula, Arnaud; Selenica, Pier; Brown, David N.Analysis of cell-free circulating tumor DNA obtained by liquid biopsy is a non-invasive approach that may provide clinically actionable information when conventional tissue biopsy is inaccessible or infeasible. Here, we followed a patient with hormone receptor-positive and human epidermal growth factor receptor (HER) 2-negative breast cancer who developed bone metastases seven years after mastectomy. We analyze...
Circulating tumor cell detection methods in renal cell carcinoma : a systematic...
Palmela Leitão, Tito; Miranda, Miguel; Polido, Joana; Morais, João; Corredeira, Patrícia; Alves, Patrícia; Ribeiro-de-Oliveira, TiagoCirculating tumor cells (CTCs) have a potential role as the missing renal cell carcinoma (RCC) biomarker. However, the available evidence is limited, and detection methods lack standardization, hindering clinical use. We performed a systematic review on CTC enrichment and detection methods, and its role as a biomarker in RCC. Full-text screening identified 54 studies. Reviewed studies showed wide heterogeneity,...
HER2 expression in circulating tumour cells isolated from metastatic breast can...
Lopes, Cláudia; Piairo, Paulina; Chícharo, Alexandre; Abalde-Cela, Sara; Pires, Liliana R.; Corredeira, Patrícia; Alves, Patrícia; Muinelo-Romay, LauraHER2 is a prognostic and predictive biomarker in breast cancer, normally assessed in tumour biopsy and used to guide treatment choices. Circulating tumour cells (CTCs) escape the primary tumour and enter the bloodstream, exhibiting great metastatic potential and representing a real-time snapshot of the tumour burden. Liquid biopsy offers the unique opportunity for low invasive sampling in cancer patients and ho...
MicroRNA‐181a restricts human γδ T cell differentiation by targeting Map3k2 and...
Gordino, Gisela; Costa Pereira, Sara; Corredeira, Patrícia; Alves, Patrícia; Costa, Luis; Gomes, Anita Q.; Silva-Santos, Bruno; Ribot, Julieγδ T cells are a conserved population of lymphocytes that contributes to anti-tumor responses through its overt type 1 inflammatory and cytotoxic properties. We have previously shown that human γδ T cells acquire this profile upon stimulation with IL-2 or IL-15, in a differentiation process dependent on MAPK/ERK signaling. Here, we identify microRNA-181a as a key modulator of human γδ T cell differentiation. We...
Expression of receptor activator of NFkB (RANK) drives stemness and resistance ...
Fernandes Gomes, Inês; Almeida, Bernardo P. De; Dâmaso, Sara; Mansinho, André; Correia, Inês; Henriques, Sara; Duarte, Raquel; Vilhais, GuilhermeThe role of RANKL-RANK pathway in progesterone-driven mammary carcinogenesis and triple negative breast cancer tumorigenesis has been well characterized. However, and despite evidences of the existence of RANK-positive hormone receptor (HR)-positive breast tumors, the implication of RANK expression in HR-positive breast cancers has not been addressed before. Here, we report that RANK pathway affects the express...