16 documents found, page 1 of 2
Continuous Production of Influenza VLPs Using IC-BEVS and Multi-Stage Bioreactors
Correia, Ricardo; Zotler, Taja; Ferraz, Francisco; Fernandes, Bárbara; Graça, Miguel; Pijlman, Gorben P.; Alves, Paula M.; Roldão, AntónioFunding Information: iNOVA4Health (UIDB/04462/2020 and UIDP/04462/2020), a program financially supported by Funda\u00E7\u00E3o para a Ci\u00EAncia e a Tecnologia (FCT)/Minist\u00E9rio da Ci\u00EAncia, Tecnologia e Ensino Superior, is acknowledged through national funds. Funding from INTERFACE Programme, through the Innovation, Technology and Circular Economy Fund (FITEC), is gratefully acknowledged. This work w...
Engineered thermoswitch in the baculovirus expression vector system for product...
van Oosten, Linda; Altenburg, Jort J.; Nowee, Gwen; Kenbeek, Dennis; Neef, Tessa; Rouw, Thomas; Tegelbeckers, Vivian I.P.; van der Heijden, JansFunding Information: We acknowledge Dr Manli Wang (Wuhan Institute of Virology, Chinese Academy of Sciences) for sharing the Ac\u0394gp41 bacmid. The authors acknowledge Dr Sandra Abbo for her valuable input on the WNV prME cassette design and Dr Astrid Bryon for analysis of the Illumina sequencing data. We thank Adrie Westphal and Simon Lindhoud (Laboratory of Biochemistry, Wageningen University) for scientifi...
The Impact of Intra-Operative Heparin on Thromboembolism and Death in a Matched...
Ribeiro, Tiago F; Correia, Ricardo; Soares Ferreira, Rita; Bastos Gonçalves, Frederico; Amaral, Carlos; Ferreira, Maria EmíliaPublisher Copyright: © 2023 The Author(s); Objective: Portuguese nationwide estimates indicate that 20% of abdominal aortic aneurysms (AAAs) are treated when ruptured. In these cases, intra-operative unfractionated heparin (UFH) usage rates vary widely. Evidence on this topic is scarce and focused on patients treated by open repair (OSR). The aim was to determine the influence of UFH on peri-operative thromboem...
Maceration of Waste Cork in Binary Hydrophilic Solvents for the Production of F...
Şen, Umut; Viegas, Catarina; Duarte, Maria Paula; Maurício, Elisabete Muchagato; Nobre, Catarina; Correia, Ricardo; Pereira, HelenaPublisher Copyright: © 2023 by the authors.; Waste-grade cork samples of Quercus cerris were subjected to maceration extraction using 7 different solvents, including pure water (I), pure acetone (II), 75% aqueous ethanol (III), 75% aqueous methanol (IV), 75% aqueous acetone (V), 50% aqueous acetone (VI), and 25% aqueous acetone (VII). The extract yields, extract compositions, as well as antioxidant and antimicr...
Dissecting insect cell heterogeneity during influenza VLP production using sing...
Silvano, Marco; Virgolini, Nikolaus; Correia, Ricardo; Clarke, Colin; Isidro, Inês A.; Alves, Paula M.; Roldão, AntónioFunding Information: This work has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No. 813453 and the grant agreement N° 730964, and by Fundação para a Ciência e a Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior (FCT/MCTES, Portugal) through the following initiatives: “Investigador FCT” Program (IF/01704/20...
Transcriptome analysis of Sf9 insect cells during production of recombinant Ade...
Virgolini, Nikolaus; Hagan, Ryan; Correia, Ricardo; Silvano, Marco; Fernandes, Sofia; Alves, Paula m.; Clarke, Colin; Roldão, António; Isidro, Inês a.The insect cell-baculovirus expression vector system (IC-BEVS) has emerged as an alternative time- and cost-efficient production platform for recombinant Adeno-associated virus (AAV) for gene therapy. However, a better understanding of the underlying biological mechanisms of IC-BEVS is fundamental to further optimize this expression system toward increased product titer and quality. Here, gene expression of Sf9...
Impact of dual-baculovirus infection on the Sf9 insect cell transcriptome durin...
Virgolini, Nikolaus; Silvano, Marco; Hagan, Ryan; Correia, Ricardo; Alves, Paula M.; Clarke, Colin; Roldão, António; Isidro, Inês A.Funding Information: The authors gratefully acknowledge the funding from the European Union's Horizon 2020 research and innovation program under the Marie Sklodowska‐Curie grant agreement no. 813453 and from Fundação para a Ciência e a Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior (FCT/MCTES, Portugal) through national funds to iNOVA4Health (UIDB/04462/2020 and UIDP/04462/2020) and the Associat...
Vaccine-induced neutralizing antibody responses to seasonal influenza virus H1N...
Mooij, Petra; Mortier, Daniella; Aartse, Aafke; Murad, Alexandre B.; Correia, Ricardo; Roldão, António; Alves, Paula M.; Fagrouch, Zahra; Eggink, DirkFunding Information: This work was supported by EU-funded project “EDUFLUVAC” (FP7-HEALTH-2013-INNOVATION-1, GA n. 602640), by Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior (FCT/MCTES, Portugal) through several initiatives (iNOVA4Health (UIDB/04462/2020 and UIDP/04462/2020), Associate Laboratory LS4FUTURE (LA/P/0087/2020), “Investigador FCT” Program (IF/01704/2014), Ex...
Towards a scalable bioprocess for rAAV production using a HeLa stable cell line
Escandell, José; Moura, Filipa; Carvalho, Sofia B.; Silva, Ricardo J.S.; Correia, Ricardo; Roldão, Antonio; Gomes-Alves, Patrícia; Alves, Paula M.Funding Information: We thank Dr. Maria João Sebastião, Dr. Mafalda Dias, Maria Gonçalves and Mariana Antunes for their helpful discussion and experimental support. This work was funded by Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior (FCT/MCTES, Portugal) through national funds to iNOVA4Health (UIDB/04462/2020 and UIDP/04462/2020), the Associate Laboratory LS4FUTURE (...
Water Peel-Off Transfer of Electronically Enhanced, Paper-Based Laser-Induced G...
Pinheiro, Tomás; Correia, Ricardo; Morais, Maria; Coelho, João; Fortunato, Elvira; Sales, M. Goreti F.; Marques, Ana C.; Martins, RodrigoPublisher Copyright: © 2022 American Chemical Society.; Laser-induced graphene (LIG) has gained preponderance in recent years, as a very attractive material for the fabrication and patterning of graphitic structures and electrodes, for multiple applications in electronics. Typically, polymeric substrates, such as polyimide, have been used as precursor materials, but other organic, more sustainable, and accessib...