43 documents found, page 1 of 5
Viscous microcapsules as microbioreactors to study mesenchymal stem/stromal cel...
Ghasemzadeh-Hasankolaei, Maryam; Miranda, João M.; Correia, Clara R.; Mano, João F.It is essential to design a multifunctional well-controlled platform to transfermechanical cues to the cells in different magnitudes. This study introduces aplatform, a miniaturized bioreactor, which enables to study the effect of shearstress in microsized compartmentalized structures. In this system, thewell-established cell encapsulation system of liquefied capsules (LCs) is usedas microbioreactors in which t...
Close-to-native bone repair via tissue-engineered endochondral ossification app...
Nadine, Sara; Fernandes, Inês J.; Correia, Clara R.; Mano, João F.In order to solve the clinical challenges related to bone grafting, several tissue engineering (TE) strategies have been proposed to repair critical-sized defects. Generally, the classical TE approaches are designed to promote bone repair via intramembranous ossification. Although promising, strategies that direct the osteogenic differentiation of mesenchymal stem/stromal cells are usually characterized by a la...
Engineering immunomodulatory hydrogels and cell-laden systems towards bone rege...
Nadine, Sara; Correia, Clara R.; Mano, João F.The well-known synergetic interplay between the skeletal and immune systems has changed the design of advanced bone tissue engineering strategies. The immune system is essential during the bone lifetime, with macrophages playing multiple roles in bone healing and biomaterial integration. If in the past, the most valuable aspect of implants was to avoid immune responses of the host, nowadays, it is well-establis...
Bioengineered hierarchical bonelike compartmentalized microconstructs using nan...
Bjørge, Isabel M.; Sousa, Bárbara M. de; Patrício, Sónia G.; Silva, Ana Sofia; Nogueira, Liebert P.; Santos, Lúcia F.; Vieira, Sandra I.Fabrication of vascularized large-scale constructs for regenerative medicine remains elusive since most strategies rely solely on cell self-organization or overly control cell positioning, failing to address nutrient diffusion limitations. We propose a modular and hierarchical tissue-engineering strategy to produce bonelike tissues carrying signals to promote prevascularization. In these 3D systems, disc-shaped...
The therapeutic potential of hematopoietic stem cells in bone regeneration
Oliveira, Claudia S.; Carreira, Mariana; Correia, Clara R.; Mano, João F.The repair process of bone fractures is a complex biological mechanism requiring the recruitment and in situ functionality of stem/stromal cells from the bone marrow (BM). BM mesenchymal stem/stromal cells have been widely explored in multiple bone tissue engineering applications, whereas the use of hematopoietic stem cells (HSCs) has been poorly investigated in this context. A reasonable explanation is the fac...
Hipster microcarriers: exploring geometrical and topographical cues of non-sphe...
Bjørge, Isabel M.; Correia, Clara R.; Mano, João F.Structure and organisation are key aspects of the native tissue environment, which ultimately condition cell fate via a myriad of processes, including the activation of mechanotransduction pathways. By modulating the formation of integrin-mediated adhesions and consequently impacting cell contractility, engineered geometrical and topographical cues may be introduced to activate downstream signalling and ultimat...
Bioengineering the human bone marrow microenvironment in liquefied compartments...
Oliveira, Cláudia S.; Nadine, Sara; Gomes, Maria C.; Correia, Clara R.; Mano, João F.Recreating the biological complexity of living bone marrow (BM) in a single in vitro strategy has faced many challenges. Most bioengineered strategies propose the co-culture of BM cellular components entrapped in different matrices limiting their migration and self-organization capacity or in open scaffolds enabling their escaping. We propose a methodology for fabricating a "human bone marrow-in-a-liquefied-cap...
Liquefied microcapsules compartmentalizing macrophages and umbilical cord-deriv...
Nadine, Sara; Fernandes, Inês; Patrício, Sónia G.; Correia, Clara R.; Mano, João F.Extraordinary capabilities underlie the potential use of immune cells, particularly macrophages, in bone tissue engineering. Indeed, the depletion of macrophages during bone repair often culminates in disease scenarios. Inspired by the native dynamics between immune and skeletal systems, this work proposes a straightforward in vitro method to bioengineer biomimetic bone niches using biological waste. For that, ...
3D-bioprinted constructs that breathe
Correia, Clara R.; Mano, João F.An old but still hot topic in tissue engineering (TE) is the establishment of efficient vascularization networks proving fine, controlled, and long-term distribution of oxygen and nutrients. Combining elegant three-dimensional (3D) fabrication techniques with unconventional living microorganisms, namely photosynthetic species, complex 3D-printed TE constructs are proposed by Maharjan et al. enabling to provide ...
An immunomodulatory miniaturized 3D screening platform using liquefied capsules
Nadine, Sara; Correia, Clara R.; Mano, João F.A critical determinant of successful clinical outcomes is the host's response to the biomaterial. Therefore, the prediction of the immunomodulatory bioperformance of biomedical devices following implantation is of utmost importance. Herein, liquefied capsules are proposed as immunomodulatory miniaturized 3D platforms for the high-content combinatorial screening of different polymers that could be used generical...