52 documents found, page 1 of 6

Distribution of Chlamydia trachomatis ompA-genotypes over three decades in Port...

Objectives: Chlamydia trachomatis is classified into 15 major genotypes, A to L3, based on the diversity of ompA gene. Here, we evaluated and characterised the distribution and diversity of ompA-genotypes over 32 years (1990–2021) in Portugal. Methods: The collection of the Portuguese National Reference Laboratory for Sexually Transmitted Infections includes 5824 C. trachomatis-positive samples that were succes...

Surveying genetic markers of antibiotic resistance and genomic background in Ch...

Objectives: To survey genetic markers of potential antimicrobial resistance (AMR) to macrolides and fluoroquinolones among Chlamydia trachomatis–positive samples from the collection of the Portuguese National Reference Laboratory for Sexually Transmitted Infections (STIs), and explore a multiplex PCR approach coupled with NGS to provide complementary information regarding a strain’s genomic backbone. Methods: A...

Lymphogranuloma venereum (LGV) ompA-subvariants of the Portuguese collection of...

Background: Lymphogranuloma venereum (LGV) is a sexually transmitted infection caused by Chlamydia trachomatis ompA-genotypes L1–L3, with increasing numbers of detected cases across Europe. Here, we analysed diversity and temporal distribution of the LGV ompA-subvariants detected in Portugal between 2007 and 2023, in order to better understand the dissemination and diversification landscape of LGV strains. Meth...

Infeções por Chlamydia trachomatis e Neisseria gonorrhoeae: resultados do diagn...

Chlamydia trachomatis e Neisseria gonorrhoeae são responsáveis pelas duas infeções sexualmente transmissíveis (IST) curáveis mais comuns em todo o mundo. Sendo frequentemente assintomáticas, o rastreio laboratorial destas IST é fundamental, para que possa ser efetuado o tratamento adequado que evite o desenvolvimento de sequelas clínicas graves e para quebrar cadeias de transmissão. O presente estudo teve por o...

Orogenital and anal infection by Chlamydia trachomatis, Neisseria gonorrhoeae, ...

Background: Men who have sex with men (MSM) are at risk for sexually transmitted infections (STIs), but more data on extragenital carriage are needed. Aim: We assessed the genital and extragenital prevalence of bacterial and other STIs in MSM in a Lisbon sexual health clinic. Methods: We screened oral, anal, and urine samples of MSM visiting the GAT-CheckpointLX clinic June 2017-December 2021 for Chlamydia trac...

Antioxidant and antimicrobial properties of PLA based active packaging with pom...

Active food packaging’ primary goal is to extend foodstuffs’ shelf life, through a dynamic and continuous interaction between the package and the packaged food. In an emission active packaging, the objective is the gradual release of antioxidant and/or antimicrobial compounds into the food surface, to delay the natural foods’ degradation. The active compounds can be extracted from several sources, such as aroma...

Using B15 in vehicles on real on-road circumstances - A case study

Extending beef meat shelf life: an approach using active food packaging with po...

More than 50% of the pomegranate fruit (Punica granatum L.) is not considered edible, being composed by peels (50%) and seeds (10%). Pomegranate peels represent an excellent source of bioactive compounds with excellent antioxidant and antimicrobial activities due to the peels’ high content in phenolic compounds, specially punicalagin (A+B) and ellagic acid. The aim of the present study was to evaluate the poten...

Transcontinental dissemination of the L2b/D-Da recombinant Chlamydia trachomati...

Previously, we identified a Chlamydia trachomatis Lymphogranuloma venereum (LGV) recombinant strain possessing a unique non-LGV ompA genotype. Here, culture-independent genome sequencing confirms its circulation in Europe, Middle East and North America, and unveils genetic evidence of emergence of antibiotic resistance. Multi-country and systematic molecular surveillance is needed.

Transcontinental Dissemination of the L2b/D-Da Recombinant Chlamydia trachomati...

Previously, we identified a Chlamydia trachomatis lymphogranuloma venereum (LGV) recombinant strain possessing a non-LGV ompA genotype. Here, culture-independent genome sequencing confirms its circulation in Europe, Middle East, and North America, and unveils emergence of antibiotic resistance. Broad surveillance is needed.