21 documents found, page 1 of 3

An electronic tongue as a classifier tool for assessing perfume olfactory famil...

The identification of more than three perfumes is difficult and no analytical tool can completely replace the human olfactory system for fragrance classification. Indeed, no analytical system can mimic the human fragrance perception, being the recognition of perfume aroma patterns by conventional or sensor-based analytical tools a challenging task. For the perfume sector, the possibility of applying fast, cost-...

Mapping Salmonella typhimurium pathways using ¹³C metabolic flux analysis

In the last years, Salmonella has been extensively studied not only due to its importance as a pathogen, but also as a host to produce pharmaceutical compounds. However, the full exploitation of Salmonella as a platform for bioproduct delivery has been hampered by the lack of information about its metabolism. Genome-scale metabolic models can be valuable tools to delineate metabolic engineering strategies as lo...

Análise de fluxos metabólicos com substrato isotopicamente marcado (13C-MFA) em...

Linhagens atenuadas de Salmonella têm sido estudadas para produção e veiculação de substâncias com fins terapêuticos. Modelos metabólicos à escala genômica são ferramentas importantes no desenvolvimento de estratégias de engenharia metabólica. Este trabalho teve por objetivo obter dados experimentais para aprimorar o modelo metabólico e aprofundar o conhecimento do metabolismo de S. typhimurium (St). Foram real...

Reconstruction and validation of iTR383, a genome-scale metabolic model for Hel...

Helicobacter pylori 26695, a highly pathogenic bacterium, is a human gastric epithelia colonizer, correlated with the development of duodenal and gastric ulcers, and gastric cancer worldwide. Its genome has been previously sequenced and annotated, and two genome-scale metabolic models have been developed; however, since their publication, vast amounts of data and new methodologies have been developed. In order ...

Salmonella typhimurium and Escherichia coli dissimilarity: closely related bact...

Live attenuated strains of Salmonella typhimurium have been extensively investigated as vaccines for a number of infectious diseases. However, there is still little information available concerning aspects of their metabolism. S. typhimurium and Escherichia coli show a high degree of similarity in terms of their genome contents and metabolic networks. However, this work presents experimental evidence showing th...

Analysis of Salmonella typhimurium pathways and metabolic model improvement

Live attenuated strains of Salmonella typhimurium have been extensively investigated as vaccines for several infectious diseases. However, a better knowledge of S. typhimurium metabolism is required to develop protocols to improve bioprocesses for the production of biotechnological products in large scale. Currently, genome scale metabolic models are important tools for better understanding the phenotypic behav...

Uncovering the metabolic capacities of H. pylori 26695 using 13C labeling exper...

The determination of nutritional requirements of pathogenic organisms is of great significance for understanding host-pathogen interactions. Despite the knowledge obtained so far concerning amino acid requirements in H. pylori, it is still unclear which are the metabolic pathways used for biosynthesis and catabolism. Thus, information on the carbon flow in this organism is required. Glutamate is a very importan...

Reconstruction and validation of a genome-scale metabolic model for Helicobacte...

Dietary sugars analysis: quantification of fructooligossacharides during fermen...

In this work, a simple chromatographic method is proposed and in-house validated for the quantification of total and individual fructooligossacharides (e.g., 1-kestose, nystose, and 1F-fructofuranosylnystose). It was shown that a high-performance liquid chromatography with refractive index detector could be used to monitor the dynamic of fructooligossacharides production via sucrose fermentation using Aspergill...

Systems analysis of metabolism in Helicobacter pylori

Helicobacter pylori is associated with gastric diseases, such as gastritis, peptic and duodenal ulcers, mucosa associated lymphoid tissue lymphoma and gastric adenocarcinomas. Despite more than half of the global population being infected with this bacterium, not all individuals will develop clinical symptoms. Nevertheless, its association with gastric cancer, the high infection rate, as well as the failures on...