3 documents found, page 1 of 1
Impact of Conventional and Potential New Metal-Based Drugs on Lipid Metabolism ...
Bispo, Daniela S. C.; Correia, Marlene; Carneiro, Tatiana J.; Martins, Ana S.; Reis, Aliana A. N.; Carvalho, Ana L. M. Batista de; Marques, Maria P. M.This work investigated the mechanisms of action of conventional drugs, cisplatin and oxaliplatin, and the potentially less deleterious drug Pd2Spermine (Spm) and its Pt(II) analog, against osteosarcoma MG-63 cells, using nuclear-magnetic-resonance metabolomics of the cellular lipidome. The Pt(II) chelates induced different responses, namely regarding polyunsaturated-fatty-acids (increased upon cisplatin), sugge...
NMR metabolomics assessment of osteogenic differentiation of adipose-tissue-der...
Bispo, Daniela S. C.; Jesus, Catarina S. H.; Correia, Marlene; Ferreira, Filipa; Bonifazio, Giulia; Goodfellow, Brian J.; Oliveira, Mariana B.This Article presents, for the first time to our knowledge, an untargeted nuclear magnetic resonance (NMR) metabolomic characterization of the polar intracellular metabolic adaptations of human adipose-derived mesenchymal stem cells during osteogenic differentiation. The use of mesenchymal stem cells (MSCs) for bone regeneration is a promising alternative to conventional bone grafts, and untargeted metabolomics...
Endo- and exometabolome crosstalk in mesenchymal stem cells undergoing osteogen...
Bispo, Daniela S. C.; Michálková, Lenka; Correia, Marlene; Jesus, Catarina S. H.; Duarte, Iola F.; Goodfellow, Brian J.; Oliveira, Mariana B.This paper describes, for the first time to our knowledge, a lipidome and exometabolome characterization of osteogenic differentiation for human adipose tissue stem cells (hAMSCs) using nuclear magnetic resonance (NMR) spectroscopy. The holistic nature of NMR enabled the time-course evolution of cholesterol, mono- and polyunsaturated fatty acids (including ω-6 and ω-3 fatty acids), several phospholipids (phosph...