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Heterotrophic cultivation of Chlorella vulgaris yellow mutant on sidestreams: M...

Trovão, Mafalda; Barros, Ana; Machado, Adriana; Reis, Ana; Pedroso, Humberto; Espírito Santo, Gonçalo; Correia, Nádia; Costa, Monya; Ferreira, Sara

Microalgal protein is a promising feedstock to complement and/or replace other protein sources. Besides requiring less land and water usage, microalgae production is a more sustainable process, especially if industrial sidestreams are used as nutrient sources. Additionally, the heterotrophic cultivation of microalgae, such as Chlorella vulgaris, enables the achievement of much higher biomass productivity and lo...


Improving the heterotrophic media of three chlorella vulgaris mutants toward op...

Trovão dos Santos, Mafalda; Cunha, Miguel; Santo, Gonçalo Espírito; Pedroso, Humberto; Reis, Ana; Barros, Ana; Correia, Nádia; Schüler, Lisa

The high production costs and unappealing sensory properties still limit the widespread commercialization of microalgae feedstocks. Therefore, this work focused on fine-tuning the heterotrophic medium composition to cultivate novel green, yellow, and white Chlorella vulgaris mutant strains. Screening assays were carried out to select the most significant factors, and different nutrient concentrations were optim...


Oxyfluorfen: a novel metabolic inhibitor to select microalgal chlorophyll-defic...

Trovão dos Santos, Mafalda; Cardoso, Lucas; Schüler, Lisa; Machado, Adriana; Santo, Gonçalo Espírito; Pedroso, Humberto; Reis, Ana; Barros, Ana

Nowadays, there is an increasing demand for novel feedstocks and alternative protein sources to meet global needs. Because of their rich nutritional profiles and high protein contents, microalgae-based food products and supplements are being developed. Nonetheless, these products present organoleptic characteristics such as taste, smell and colour that are often considered unpleasant by human and animal consume...


Isolation and selection of protein-rich mutants of chlorella vulgaris by fluore...

Trovão dos Santos, Mafalda; Schüler, Lisa; Pedroso, Humberto; Reis, Ana; Santo, Gonçalo Espírito; Barros, Ana; Correia, Nádia; Ribeiro, Joana

Microalgae are a promising feedstock with proven biostimulant activity that is enhanced by their biochemical components (e.g., amino acids and phytohormones), which turns them into an appealing feedstock to reduce the use of fertilisers in agriculture and improve crop productivity and resilience. Thus, this work aimed to isolate protein-rich microalgal mutants with increased biostimulant activity. Random mutage...


Relatório de Estágio Curricular – Comunilog Consulting, Lda (Guarda)

Correia, Nádia

Com o término da licenciatura em Gestão torna-se necessário colocar em prática aquilo que foi parte do processo de aprendizagem ao longo dos 3 anos académicos. O fim da licenciatura em Gestão na Escola Superior de Tecnologia e Gestão (ESTG) do Instituto Politécnico da Guarda (IPG) fica marcado pelo estágio final para a conclusão do curso perfazendo 400 horas. O presente relatório é elaborado respeitando o regul...


Nonthermal high-pressure microalgae extracts: A new source of natural ingredien...

Garcia, Mónica P.; Regueiras, Ana; Lopes, Graciliana; Matos, Gabriela; Silva, Lucília P. da; Cerqueira, Mariana T.; Cardoso, Helena; Correia, Nádia

Microalgae are one of the most prominent sources of ingredients for the cosmetic industry not only due to their diversity but, importantly, due to the low environmental impact in production and extraction. A sustainable extraction process requires the use of effective and environmentally friendly solvents that guarantee an extract with a relevant content and in high yields. In this work, Chlorella vulgaris, Nan...


Heterotrophic and Photoautotrophic Media Optimization Using Response Surface Me...

Correia, Nádia; Pereira, Hugo; Schulze, Peter; Costa, Monya M.; Santo, Gonçalo E.; Guerra, Inês; Trovão, Mafalda; Barros, Ana; Cardoso, Helena

ABSTRACT: The nutritional requirements of novel microalgal strains are key for their effective cultivation and metabolite content. Therefore, the optimization of heterotrophic and photoautotrophic culture media is crucial for novel Chlorococcum amblystomatis growth. Heterotrophic and photoautotrophic biomass samples were characterized to identify the differences between their heterotrophic and photoautotrophic ...

Date: 2023   |   Origin: Repositório do LNEG

Heterotrophic and photoautotrophic media optimization using response surface me...

Correia, Nádia; Pereira, Hugo; Schulze, Peter S.C.; Costa, Monya; Santo, Gonçalo E.; Guerra, Inês; Trovão dos Santos, Mafalda; Barros, Ana

The nutritional requirements of novel microalgal strains are key for their effective cultivation and metabolite content. Therefore, the optimization of heterotrophic and photoautotrophic culture media is crucial for novel Chlorococcum amblystomatis growth. Heterotrophic and photoautotrophic biomass samples were characterized to identify the differences between their heterotrophic and photoautotrophic biomass co...


Effects of outdoor and indoor cultivation on the polar lipid composition and an...

Couto, Daniela; Conde, Tiago A.; Melo, Tânia; Neves, Bruna; Costa, Margarida; Cunha, Pedro; Guerra, Inês; Correia, Nádia; Silva, Joana T.; Pereira, Hugo

Nannochloropsis is a genus of eicosapentaenoic acid-rich microalgae with high levels of value-added polar lipids. However, the polar lipid composition of microalgal biomass is highly dependent on culture conditions (e.g., light or temperature), which are significantly different under indoor and outdoor culture conditions. In this study, we sought to investigate the plasticity of the polar lipid profile of a mar...


Isolation, Identification and Biotechnological Applications of a Novel, Robust,...

Correia, Nádia; Pereira, Hugo; Silva, Joana T.; Santos, Tamára; Soares, Maria; Sousa, Carolina B.; Schüler, Lisa M.; Costa, Margarida; Varela, João

Bioprospection of novel autochthonous strains is key to the successful industrial-scale production of microalgal biomass. A novel Chlorococcum strain was recently isolated from a pond inside the industrial production facility of Allmicroalgae (Leiria, Portugal). Phylogenetic analysis based on 18S ribosomal ribonucleic acid (rRNA) gene sequences suggests that this isolate is a novel, free-living Oophila amblysto...


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