34 documents found, page 1 of 4
Encapsulation of pristine and silica-coated human adipose-derived mesenchymal s...
Maciel, Marta M.; Hassani Besheli, Negar; Correia, Tiago R.; Mano, João F.; Leeuwenburgh, Sander C. G.Colloidal gels assembled from gelatin nanoparticles (GNPs) as particulate building blocks show strong promise to solve challenges in cell delivery and biofabrication, such as low cell survival and limited spatial retention. These gels offer evident advantages to facilitate cell encapsulation, but research on this topic is still limited, which hampers our understanding of the relationship between the physicochem...
In-bath 3D printing of anisotropic shape-memory cryogels functionalized with bo...
Castanheira, Edgar J.; Monteiro, Luís P. G.; Gaspar, Vítor M.; Correia, Tiago R.; Rodrigues, João M. M.; Mano, João F.Cryogels exhibit unique shape memory with full recovery and structural stability features after multiple injections. These constructs also possess enhanced cell permeability and nutrient diffusion when compared to typical bulk hydrogels. Volumetric processing of cryogels functionalized with nanosized units has potential to widen their biomedical applications, however this has remained challenging and relatively...
Bioinstructive liquefied pockets in hierarchical hydrogels and bioinks
Ghasemzadeh-Hasankolaei, Maryam; Correia, Tiago R.; Mano, João F.This study proposes a novel, versatile, and modular platform for constructing porous and heterogeneous microenvironments based on the embedding of liquefied-based compartments in hydrogel systems. Using a bottom-up approach, microgels carrying the necessary cargo components, including cells and microparticles, are combined with a hydrogel precursor to fabricate a hierarchical structured (HS) system. The HS syst...
Microparticles orchestrating cell fate in bottom-up approaches
Maciel, Marta M.; Correia, Tiago R.; Henriques, Mariana; Mano, J. F.The modulation of cells in tissue formation is still one of the hardest tasks to achieve in Tissue Engineering. To control the cell response when undergoing their normal functions such as adhesion, differentiation, assembly, or maturation is vital the development of more successful solutions. Herein, we discuss how microparticles are being overlooked in their potential for controlling the cellular response. Unt...
Partial coated stem cells with bioinspired silica as new generation of cellular...
Maciel, Marta M.; Correia, Tiago R.; Gaspar, Vítor M.; Rodrigues, João M. M.; Choi, Insung S.; Mano, J. F.The manipulation of cell surfaces in anchorage-dependent cells can help overcome difficulties presented during cell handling and application. Silica-based protective mechanisms existing in free-floating microorganisms may be used as inspiration for sustaining human cell survival in suspension. Gathering on this, herein human adipose-derived mesenchymal stem cells (hASCs) are partially coated with a hard silica ...
Bioinstructive Layer-by-Layer-Coated Customizable 3D Printed Perfusable Microch...
Sousa, Cristiana F. V.; Saraiva, Catarina A.; Correia, Tiago R.; Pesqueira, Tamagno; Patrício, Sónia G.; Rial-Hermida, Maria Isabel; Borges, JoãoThe development of complex and large 3D vascularized tissue constructs remains the major goal of tissue engineering and regenerative medicine (TERM). To date, several strategies have been proposed to build functional and perfusable vascular networks in 3D tissue-engineered constructs to ensure the long-term cell survival and the functionality of the assembled tissues after implantation. However, none of them ha...
Partial coated stem cells with bioinspired silica as new generation of cellular...
Maciel, Marta M.; Correia, Tiago R.; Gaspar, Vítor M.; Rodrigues, João M. M.; Choi, Insung S.; Mano, João F.The manipulation of cell surfaces in anchorage-dependent cells can help overcome difficulties presented during cell handling and application. Silica-based protective mechanisms existing in free-floating microorganisms may be used as inspiration for sustaining human cell survival in suspension. Gathering on this, herein human adipose-derived mesenchymal stem cells (hASCs) are partially coated with a hard silica ...
Natural origin biomaterials for 4D bioprinting tissue‐like constructs
Costa, Patrícia D. C.; Costa, Dora C. S.; Correia, Tiago R.; Gaspar, Vítor M.; Mano, João F.Leveraging 4D biofabrication for engineering biomimetic living constructs is rapidly emerging as a valuable strategy for recapitulating native tissue dynamics, via on-demand stimuli, or in a naturally evolving mode. Carefully selecting smart materials with suitable responsiveness and cell-supporting functionalities is crucial to take full operational advantage of this next-generation technology. Recent endeavor...
Thin silica-based microsheets with controlled geometry
Maciel, Marta; Patrício, Sónia G.; Borges, João; Levkin, Pavel A.; Correia, Tiago R.; Mano, J. F.A high demand for materials with defined geometry and size is required in a wide range of fields. Inorganic compounds, especially silica-based, arise as a cheap source and chemically flexible for the purpose. Silica display unique properties, like easy functionalization and good optical features making an interesting material to manipulate. In this work, we developed a method to create thin silica microsheets w...
Novel biodegradable laminarin microparticles for biomedical applications
Castanheira, Edgar J.; Correia, Tiago R.; Rodrigues, João M. M.; Mano, João F.Fabrication of biocompatible polymeric carriers for a sustained/controlled drug-delivery have been extensively explored over the years. Furthermore, systems based on polymers from natural origins exceed conventional polymers in biocompatibility, biodegradability and cost efficiency. Polysaccharides are one of the most common biopolymers found in nature and they can achieve a high degree of complexity and fine b...