13 documents found, page 1 of 2
How to dissect viral infections and their interplay with the host-proteome by i...
Santos, Hugo M.; Carvalho, Luís B.; Lodeiro, Carlos; Martins, Gonçalo; Gomes, Inês L.; Antunes, Wilson D.T.; Correia, Vanessa; Almeida-Santos, Maria M.The capabilities of bioanalytical mass spectrometry to (i) detect and differentiate viruses at the peptide level whilst maintaining high sample throughput and (ii) to provide diagnosis and prognosis for infected patients are presented as a tutorial in this work to aid analytical chemists and physicians to gain insights into the possibilities offered by current high-resolution mass spectrometry technology and bi...
NS1 protein as a novel anti-influenza target: Map-and-mutate antiviral rational...
Trigueiro-Louro, João; Santos, Luís A.; Almeida, Filipe; Correia, Vanessa; Brito, Rui M.M.; Rebelo-de-Andrade, HelenaInfluenza NS1 is a promising anti-influenza target, considering its conserved and druggable structure, and key function in influenza replication and pathogenesis. Notwithstanding, target identification and validation, strengthened by experimental data, are lacking. Here, we further explored our previously designed structure-based antiviral rationale directed to highly conserved druggable NS1 regions across a br...
Unlocking COVID therapeutic targets: A structure-based rationale against SARS-C...
Trigueiro-Louro, João; Correia, Vanessa; Figueiredo-Nunes, Inês; Gíria, Marta; Rebelo-de-Andrade, HelenaThere are no approved target therapeutics against SARS-CoV-2 or other beta-CoVs. The beta-CoV Spike protein is a promising target considering the critical role in viral infection and pathogenesis and its surface exposed features. We performed a structure-based strategy targeting highly conserved druggable regions resulting from a comprehensive large-scale sequence analysis and structural characterization of Spi...
To hit or not to hit: large-scale sequence analysis and structure characterizat...
Trigueiro-Louro, João M.; Correia, Vanessa; Santos, Luís A.; Guedes, Rita C.; Brito, Rui M.M.; Rebelo-de-Andrade, HelenaInfluenza NS1 protein is among the most promising novel druggable anti-influenza target, based on its structure; multiple interactions; and global function in influenza replication and pathogenesis. Notwithstanding, drug development guidance based on NS1 structural biology is lacking. Here, we design a promising strategy directed to highly conserved druggable regions as a result of an exhaustive large-scale seq...
Molecular footprints of selective pressure in the neuraminidase gene of current...
Correia, Vanessa; Abecasis, Ana B.; Rebelo-de-Andrade, HelenaInfluenza neuraminidase (NA) is under selective pressure (SP) of both host immune system and drug use. Here, we assembled large datasets of NA sequences of worldwide circulating viruses to estimate the global and site-specific SP acting on all current subtypes/lineages of human influenza NA. An overall negative SP of similar magnitude and a prevalence of negatively selected sites were observed for all subtypes/...
Variantes genéticas do gene IFITM3 revelam um potencial modificador da gravidad...
David, Susana; Correia, Vanessa; Antunes, Liliana; Faria, Ricardo; Ferrão, José; Faustino, Paula; Nunes, Baltazar; Maltez, Fernando; Lavinha, JoãoAs epidemias de gripe representam um sério problema de saúde pública com elevados custos económicos. Foi sugerido que um dos alelos do gene IFITM3 (rs12252-C) constitui um impor tante fator de risco de base populacional para as formas graves da gripe por infeção pelo vírus A(H1N1)pdm09. Analisámos variantes do IFITM3 associadas à gravidade da doença em doentes por tugueses (n=41). Foram identificados sete SNPs ...
Influenza virus susceptibility to antiviral drugs : drug susceptibility profili...
Correia, VanessaAntivirals play an important and decisive role in the clinical management of influenza and in the underlying reduction of related morbidity and mortality. The emergence of antiviral resistance, and particularly of transmissible resistance, poses a serious threat to public health as it could render influenza antivirals useless against circulating viruses. This is even more worrying when considering the current p...
Population genetics of IFITM3 in Portugal and Central Africa reveals a potentia...
David, Susana; Correia, Vanessa; Antunes, Liliana; Faria, Ricardo; Ferrão, José; Faustino, Paula; Nunes, Baltazar; Maltez, Fernando; Lavinha, JoãoInfluenza epidemics are a serious global public health and economic problem. The IFITM3 allele (rs12252-C) was suggested as a population-based genetic risk factor for severe influenza virus infection by A(H1N1)pdm09. We analyzed the population genetics of IFITM3 variants in the Portuguese general population (n = 200) and Central Africans (largely Angolan) (n = 148) as well as its association to influenza severi...
A cost-efficient solution: reagent comparison guide for neuraminidase inhibitio...
Louro, João; Correia, Vanessa; Rebelo de Andrade, HelenaObjectives: Compare available alternative reagents for Neuraminidase Activity Assay and Neuraminidase Inhibition Assay; Assess the phenotypic susceptibility profiles of influenza viruses from different (sub)types to the new Neuraminidase Inhibitors: laninamivir and peramivir.
Uncovering the role of positive selection in the evolutionary pathways to influ...
Correia, Vanessa; Abecasis, Ana; Rebelo de Andrade, HelenaThis study aimed at estimating the site-specific SP on the NA gene of currently circulating human influenza viruses, in order to infer the SP acting specifically on the sites associated with (H)RI by NAIs and on further sites contacting directly or indirectly with the drug (active site). The frequency of drug-resistant and (H)RI variants was determined as a secondary objective, prompted by the need of determini...