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INDUCTION AND MAINTENANCE OF EMBRYOGENIC CHARACTERISTICS OF CALLUS OF THE OIL P...

Vilela,Marlúcia Souza Pádua; Andrade,Jéssica de Castro e; Santos,Raíssa Silveira; Stein,Vanessa Cristina; Alves,Patrick Callegari Magnani Santos

ABSTRACT Large-scale oil palm propagation (Elaeis guineensis Jacq.) is difficult due to its unique apical meristem. In this context, micropropagation allows the multiplication of seedlings in vitro and the storage of germplasm elites. This study aimed to induce embryogenic calluses from leaves of oil palm plants in low concentrations of auxins and to observe the maintenance of these characteristics during in vi...

Data: 2021   |   Origem: Oasisbr

HISTOLOGICAL ANALYSIS OF INDIRECT SOMATIC EMBRYOGENESIS INDUCED FROM ROOT EXPLA...

Vilela,Marlucia Souza Pádua; Andrade,Jessica de Castro e; Santos,Raissa Silveira; Stein,Vanessa Cristina; Paiva,Luciano Vilela

ABSTRACT Oil palm is economically important as a crop with high oil production. Indirect somatic embryogenesis in oil palm requires a long time for callus induction and plant formation, and it is important to study the embryogenic potential of calli and the mechanisms of somatic embryogenesis. The aim of this study was to test different growth regulators and spermine in induction of embryogenic calli in root ex...

Data: 2019   |   Origem: Oasisbr

Morpho and Cytological Differentiation of Calli of Eucalyptus grandis x Eucalyp...

Batista,Tânia Regna; Mendonça,Evânia Galvão; Pádua,Marlúcia Souza; Stein,Vanessa Cristina; Paiva,Luciano

ABSTRACT The aim of this study was to induce and analyze embryogenic calli from two types of explants (leaves and meristems) of the hybrid Eucalyptus grandis x Eucalyptus urophylla. Leaves and meristems of plants kept in a nursery were disinfected and inoculated in Petri dishes containing MS culture medium supplemented with different concentrations of the growth regulator dicamba (1.13, 4.52, and 9.04 µM) and w...

Data: 2018   |   Origem: Oasisbr

In vitro ROOTING OF TENERA HYBRID OIL PALM (Elaeis guineensis Jacq.) PLANTS1

Pádua,Marlúcia Souza; Santos,Raíssa Silveira; Paiva,Luciano Vilela; Stein,Vanessa Cristina; Silva,Luciano Coutinho

ABSTRACT Oil palm is a woody monocot of economic importance due to high oil production from its fruits. Currently, the conventional method most used to propagate oil palm is seed germination, but success is limited by long time requirements and low germination percentage. An alternative for large-scale propagation of oil palm is the biotechnological technique of somatic embryogenesis. The rooting of plants germ...

Data: 2017   |   Origem: Oasisbr

USO DE BIORREATORES DE IMERSÃO CONTINUA, TEMPORÁRIA E MEIO DE CULTURA SEMISSÓLI...

Mendonça,Evânia Galvão; Stein,Vanessa Cristina; Carvalho,Humberto Henrique de; Santos,Breno Régis; Beijo,Luiz Alberto; Paiva,Luciano Vilela

RESUMO A micropropagação em sistemas de biorreatores é considerada como uma forma de reduzir os custos de produção por meio do escalonamento de automatização do processo. O objetivo desse trabalho foi desenvolver um protocolo eficiente de produção de mudas de Eucalyptus camaldulensis em diferentes tipos de sistema, incluindo biorreator de imersão continua e temporária. Para isso, meristemas apicais (1 mm) e mer...

Data: 2016   |   Origem: Oasisbr

Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild Pa...

Carvalho,Milene Alves de Figueiredo; Paiva,Renato; Stein,Vanessa Cristina; Herrera,Raírys Cravo; Porto,Jorge Marcelo Padovani; Vargas,Daiane Peixoto

This work studied a new protocol for organogenic calli induction and characterization of the morphology and ultrastructure of callogenesis in leaf explants of Passiflora gibertii N. E. Brown, a native passion fruit species from Brazil. Calli induction was performed in different growth conditions (light and dark), different MS medium salt concentrations (MS and MS half strength) and the presence or absence of co...

Data: 2014   |   Origem: Oasisbr

Genetic transformation of Eucalyptus camaldulensis by agrobalistic method

Mendonça,Evânia Galvão; Stein,Vanessa Cristina; Balieiro,Flávia Pereira; Lima,Carolina Delfin Fernandes; Santos,Breno Régis; Paiva,Luciano Vilela

Eucalyptus stands in the setting of worldwide forestry due to its adaptability, rapid growth, production of high-quality and low cost of wood pulp fibers. The eucalyptus convetional breeding is impaired mainlly by the long life cycle making the genetic transformation systems an important tool for this purpose. However, this system requires in vitro eficient protocols for plant induction, regeneration and seleti...

Data: 2013   |   Origem: Oasisbr

Growth curve and development of the internal calli structure of Eucalyptus cama...

Mendonça,Evânia Galvão; Paiva,Luciano Vilela; Stein,Vanessa Cristina; Pires,Marinês Ferreira; Santos,Breno Régis; Pereira,Fabricio José

The objective of this work was to elucidate the growth curve of Eucalyptus camaldulensis Dehn. calli analyzing their anatomical modifications. A sigmoid aspect of the growth curve of the calli fresh matter was observed, with five different phases (lag, exponential, linear, deceleration and decline). In the lag phase, the highest growth percentage 87%, was observed, which reduced during the evaluation period to ...

Data: 2012   |   Origem: Oasisbr

Ultrastructural calli analysis of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn

Stein,Vanessa Cristina; Paiva,Renato; Vargas,Daiane Peixoto; Soares,Fernanda Pereira; Alves,Eduardo; Nogueira,Gabriela Ferreira

Subcellular changes are relevant to understand plant organogenesis and embryogenesis in the early stages of cell development. The cytology during cell development in tissue culture is however still poorly characterized. This study aimed to characterize the ultrastructural differences related to callogenesis of anthers, ovaries, leaf and nodal segments of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn. Flower b...

Data: 2010   |   Origem: Oasisbr

Efeito de meios de cultura, concentrações de GA3 e pH sobre a germinação in vit...

Soares,Fernanda Pereira; Paiva,Renato; Stein,Vanessa Cristina; Nery,Fernanda Carlota; Nogueira,Raírys Cravo; Oliveira,Lenaldo Muniz de

A mangabeira (Hancornia speciosa Gomes) destaca-se por possuir um grande potencial como planta frutífera e produtora de borracha. As dificuldades encontradas no seu processo de propagação por meio de sementes, devido, principalmente, à baixa taxa de germinação e à recalcitrância, valorizam a busca por soluções alternativas para a produção de mudas dessa espécie, de maneira rápida e eficiente. Objetivou-se, nest...

Data: 2009   |   Origem: Oasisbr

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