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Bioactive potential and chromatographic characterization of body mucus from Por...

Cunha, M. Fernandez; Coscueta, E. R.; Brassesco, M. E.; Almada, F.; Gonçalves, D.; Pintado, M.

The body mucus in fish provides a stable physical or chemical barrier against invading pathogens. In mucus, antimicrobial peptides are secreted as a response to immune stimulation. Studies have shown antimicrobial activity against multidrug-resistant pathogens and low toxicity to eukaryotic cells. Previously, body mucus from five captive Halobatrachus didactylus individuals was collected. We aim to explore the ...


Exploring the role of human gut microbiota in the phenolic compound's metabolis...

Pais, A. C. S.; Ribeiro, T. B.; Coscueta, E. R.; Pintado, M. M.; Silvestre, A. J. D.; Santos, S. A. O.


gBIOT - Nutraceutical biopolymeric-biocatalytic microbot against gut inflammato...

Sousa, A. S.; Pintado, M. M.; Matos, R. D.; Sousa, C. C.; Machado, M. F.; Coelho, M.; Rodrigues, P. M.; Magalhães, A. M.; Coscueta, E. R.

gBiOT is a novel project that proposes micro-robotic technology for nutraceuticals and therapeutic foods for gastrointestinal tract disorders. Gastrointestinal disorders have high prevalence and morbidity, leading to neoplasia and cancer. Recent advances in nanotechnology have opened up new opportunities in the food sector, allowing for the development of health-food products containing nanosized ingredients th...


P82 Peptide fraction identification by SE-HPLC and LC-MS/MS analysis of the bod...

Cunha, M.; Coscueta, E. R.; Brassesco, M. E.; Almada, F.; Gonçalves, D.; Pintado, M. Manuela

The mucus covers the fish's body, working as a protective barrier. Besides physical protection, mucus provides molecules that protect the fish from pathogens damaging 1,2. These include antimicrobial peptides secreted in the mucus, which play an essential role in defense against microbial pathogens since these belong to the innate immune system2,3. In this study, two adult Halobatrachus didactylus individuals w...


UHPLC-DAD-MSn analysis of phenolic compounds bioavailability throughout in vitr...

Pais, A. C. S.; Coscueta, E. R.; Pintado, M. M.; Silvestre, A. J. D.; Santos, S. A. O.

Phenolic compounds, one of the most widely distributed and structural diverse plant secondary metabolites families, have been the focus of several studies due to their vast range of biological activities (such as antioxidant, anti-inflammatory and/or antiproliferative). Since they are commonly present in human diet, phenolic compounds could be responsible for human health beneficial effects, preventing some dis...


Production, characterization and ultrafiltration of bioactive extracts derived ...

Cunha, S. A.; Castro, R. de; Coscueta, E. R.; Silva, J. L. da; Pintado, M. E.

Algae are very abundant worldwide and contains compounds with high biological value, such as proteins, amino acids, antioxidants. It is noteworthy that some algae species contain protein levels similar to meat, soybean and milk. Micro and macroalgae have been studied as a source of bioactive compounds, such as bioactive peptides, with properties of interest for humans and animals. In vitro, enzymatic hydrolysis...


Bioactive peptides produced from the mussel mytilus galloprovincialis by enzyma...

Cunha, S. A.; Castro, R. de; Coscueta, E. R.; Pintado, M. E.

Bioactive peptides have been produced from several marine sources since they can exhibit positive effects for humans and animals. Mytilus galloprovincialis is highly consumed in several countries and has a meat rich in proteins, which has been described as a source of bioactive peptides with relevant properties such as antioxidant, anti-hypertensive and antimicrobial activities. Mussel commercialization generat...


Collagen-based bioactive hydrolysates production from Blue Shark skin

Brassesco, M. E.; Coscueta, E. R.; Pintado, Maria Manuela

Portugal is the country with the highest levels of fish consumption in the world with an average of 59 kg per capita year. As a result, a huge amount of fish residues is produced, of which up to 30% may be skin and bone. Is well-known that fish skin has more than 80% of its total protein content as collagen. This structural protein is present in the extracellular matrices of animals and has widely applications ...


Collagen-based bioactive hydrolysates production from blue shark skin

Brassesco, M. E.; Coscueta, E. R.; Pintado, M.

Portugal is the country with the highest levels of fish consumption in the world with an average of 59 kg per capita year. As a result, a huge amount of fish residues is produced, of which up to 30% may be skin and bone. Is well-known that fish skin has more than 80% of its total protein content as collagen. This structural protein is present in the extracellular matrices of animals and has widely applications ...


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