5 documents found, page 1 of 1
Pro-inflammatory polarization and colorectal cancer modulate alternative and in...
Wilton, J; Mendonça, FL; Pereira-Castro, I; Tellier, M; Nojima, T; Costa, AM; Freitas, J; Murphy, S; Oliveira, MJ; Proudfoot, NJ; Moreira, AMacrophages are essential cells of the immune system that alter their inflammatory profile depending on their microenvironment. Alternative polyadenylation in the 3'UTR (3'UTR-APA) and intronic polyadenylation (IPA) are mechanisms that modulate gene expression, in particular in cancer and activated immune cells. Yet, how polarization and colorectal cancer (CRC) cells microenvironment affect 3'UTR-APA and IPA in...
Hypoxia and macrophages act in concert towards a beneficial outcome in colon ca...
Martins, F; Oliveira, R; Cavadas, B; Pinto, F; Cardoso, AP; Castro, F; Sousa, B; Pinto, ML; Silva, AJ; Adão, D; Loureiro, JP; Pedro, N; Reis, RMIn colon cancer, the prognostic value of macrophages is controversial, and it is still unknown how hypoxia modulates macrophage–cancer cell crosstalk. To unravel this, co-cultures of human primary macrophages and colon cancer cells were performed at 20% and 1% O2, followed by characterization of both cellular components. Different colon cancer patient cohorts were analyzed for hypoxia and immune markers, and th...
Role of Genetic Ancestry in 1,002 Brazilian Colorectal Cancer Patients From Bar...
Durães, RO; Berardinelli, GN; Costa, AM; Scapulatempo-Neto, C; Pereira, R; Oliveira, MA; Guimarães, DP; Reis, RMBackground: Colorectal cancer (CRC) is the third most frequent and the second deadliest cancer worldwide. The ethnic structure of the population has been gaining prominence as a cancer player. The purpose of this study was to determine the genetic ancestry of Brazilian CRC patients. Moreover, we intended to interrogate its impact on patients' clinicopathological features. Methods: Retrospective observational co...
It is all about location: how to pinpoint microorganisms and their functions in...
Costa, AM; Filipe Mergulhão; Briandet, R; Azevedo, NFMultispecies biofilms represent the dominant mode of life for the vast majority of microorganisms. Bacterial spatial localization in such biostructures governs ecological interactions between different populations and triggers the overall community functions. Here, we discuss the pros and cons of fluorescence-based techniques used to decipher bacterial species patterns in biofilms at single cell level, includin...
Short-term versus long-term changes in the benthic communities of a small coast...
Félix, PM; Chainho, P; Costa, Jl; Correia, MJ; Chaves, ML; Medeiros, JP; Cabral, HN; Wouters, N; Bernardo, J; Costa, AM; Cristo, M; Silva, G; Azeda, CThe characteristic high variability and low predictability of coastal lagoons, due to strong changes in marine and freshwater inputs, make these ecosystems an interesting casestudy. The small Melides landlocked coastal lagoon in SW Portugal is a paradigmatic example, with a biological community highly stressed by these phenomena. Benthic macroinvertebrate samples were collected in 1998/99 and 2009 and each year...