5 documents found, page 1 of 1
Hypoxia and Macrophages Act in Concert Towards a Beneficial Outcome in Colon Ca...
Martins, Flávia; Oliveira, Rosa; Cavadas, Bruno; Pinto, Filipe; Cardoso, Ana Patrícia; Castro, Flávia; Sousa, Bárbara; Pinto, Marta LaranjeiroIn colon cancer, the prognostic value of macrophages is controversial, and it is still unknown how hypoxia modulates macrophage-cancer cell crosstalk. To unravel this, co-cultures of human primary macrophages and colon cancer cells were performed at 20% and 1% O2, followed by characterization of both cellular components. Different colon cancer patient cohorts were analyzed for hypoxia and immune markers, and th...
WNK2 inhibits autophagic flux in human glioblastoma cell line
Alves, Ana Laura Vieira; Costa, Angela Margarida; Martinho, Olga; da Silva, Vinicius Duval; Jordan, Peter; Silva, Viviane Aline Oliveira; Reis, R. M.Autophagy is a cell-survival pathway with dual role in tumorigenesis, promoting either tumor survival or tumor death. <i>WNK2</i> gene, a member of the WNK (with no lysine (K)) subfamily, acts as a tumor suppressor gene in gliomas, regulating cell migration and invasion; however, its role in autophagy process is poorly explored. The <i>WNK2</i>-methylated human glioblastoma cell line A172 WT (wild type) was com...
WNK2 Inhibits Autophagic Flux in Human Glioblastoma Cell Line
Alves, Ana Laura Vieira; Costa, Angela Margarida; Martinho, Olga; da Silva, Vinicius Duval; Jordan, Peter; Silva, Viviane Aline OliveiraAutophagy is a cell-survival pathway with dual role in tumorigenesis, promoting either tumor survival or tumor death. WNK2 gene, a member of the WNK (with no lysine (K)) subfamily, acts as a tumor suppressor gene in gliomas, regulating cell migration and invasion; however, its role in autophagy process is poorly explored. The WNK2-methylated human glioblastoma cell line A172 WT (wild type) was compared to trans...
Silencing of the tumor suppressor gene WNK2 is associated with upregulation of ...
Costa, Angela Margarida; Pinto, Filipe; Martinho, Olga; Oliveira, Maria José; Jordan, Peter; Reis, R. M.Matrix metalloproteinases (MMPs) are proteolytic enzymes that degrade extracellular matrix (ECM), thus assisting invasion. Upregulation of MMPs, frequently reported in gliomas, is associated with aggressive behavior. WNK2 is a tumor suppressor gene expressed in normal brain, and silenced by promoter methylation in gliomas. Patients without WNK2 exhibited poor prognosis, and its downregulation was associated wit...
Silencing of WNK2 is associated with upregulation of MMP2 and JNK in gliomas
Costa, Angela Margarida; Pinto, Filipe; Martinho, Olga; Oliveira, Maria José; Jordan, Peter; Reis, R. M.Matrix metalloproteinases (MMPs) are proteolytic enzymes that degrade extracellular matrix (ECM), thus assisting invasion. Upregulation of MMPs, frequently reported in gliomas, is associated with aggressive behavior. WNK2 is a tumor suppressor gene expressed in normal brain, and silenced by promoter methylation in gliomas. Patients without WNK2 exhibited poor prognosis, and its downregulation was associated wit...