8 documents found, page 1 of 1
Bioactive self-regulated liquified microcompartments to bioengineer bone-like m...
Pinho, Ana R.; Gomes, Maria C.; Costa, Dora C. S.; Mano, João F.Designing a microenvironment that drives autonomous stromal cell differentiation toward osteogenesis while recapitulating the complexity of bone tissue remains challenging. In the current study, bone-like microtissues are created using electrohydrodynamic atomization to form two distinct liquefied microcapsules (mCAPs): i) hydroxypyridinone (HOPO)-modified gelatin (GH mCAPs, 7.5% w/v), and ii) HOPO-modified gel...
Biomimetic adhesive micropatterned hydrogel patches for drug release
Amaral, Katia R.; Silva, A. Sofia; Santos, Lúcia F.; Castanheira, Edgar J.; Mendes, Maria C.; Costa, Dora C. S.; Rodrigues, João M. M.; Marto, JoanaThe optimized physical adhesion between bees' leg hairs and pollen grains-whereby the latter's diameter aligns with the spacing between the hairs-has previously inspired the development of a biomimetic drug dressing. Combining this optimized process with the improved natural mussels' adhesion in wet environments in a dual biomimetic process, it is herein proposed the fabrication of a natural-derived micropatter...
Bioinspired oxidation-resistant catechol-like sliding ring polyrotaxane hydrogels
Rial-Hermida, M. Isabel; Costa, Dora C. S.; Jiang, Lan; Rodrigues, João M. M.; Ito, Kohzo; Mano, João F.Adaptable hydrogels have been used in the biomedical field to address several pathologies, especially those regarding tissue defects. Here, we describe unprecedented catechol-like functionalized polyrotaxane (PR) polymers able to form hydrogels. PR were functionalized with the incorporation of hydroxypyridinone (HOPO) moieties into the polymer backbone, with a degree of substitution from 4 to 22%, depending on ...
A supramolecular injectable methacryloyl chitosan-tricine-based hydrogel with 3...
Ouro, Pedro M. S.; Costa, Dora C. S.; Amaral, Adérito J. R.; Mano, João F.Printable hydrogels have attracted significant attention as versatile, tunable, and spatiotemporally controlled biomaterials for tissue engineering (TE) applications. Several chitosan-based systems are reported presenting low or no solubility in aqueous solutions at physiological pH. Herein, a novel neutrally charged, biomimetic, injectable, and cytocompatible dual-crosslinked (DC) hydrogel system based on a do...
Universal strategy for designing shape memory hydrogels
Costa, Dora C. S.; Costa, Patrícia D. C.; Gomes, Maria C.; Chandrakar, Amit; Wieringa, Paul A.; Moroni, Lorenzo; Mano, João F.Smart polymeric biomaterials have been the focus of many recent biomedical studies, especially those with adaptability to defects and potential to be implanted in the human body. Herein, we report a versatile and straightforward method to convert non-thermoresponsive hydrogels into thermoresponsive systems with shape memory ability. As a proofof-concept, a thermoresponsive polyurethane mesh was embedded within ...
Natural origin biomaterials for 4D bioprinting tissue‐like constructs
Costa, Patrícia D. C.; Costa, Dora C. S.; Correia, Tiago R.; Gaspar, Vítor M.; Mano, João F.Leveraging 4D biofabrication for engineering biomimetic living constructs is rapidly emerging as a valuable strategy for recapitulating native tissue dynamics, via on-demand stimuli, or in a naturally evolving mode. Carefully selecting smart materials with suitable responsiveness and cell-supporting functionalities is crucial to take full operational advantage of this next-generation technology. Recent endeavor...
Design of protein-based liquefied cell-laden capsules with bioinspired adhesion...
Gomes, Maria C.; Costa, Dora C. S.; Oliveira, Cláudia S.; Mano, João F.Platforms with liquid cores are extensively explored as cell delivery vehicles for cell-based therapies and tissue engineering. However, the recurrence of synthetic materials can impair its translation into the clinic. Inspired by the adhesive proteins secreted by mussels, liquefied capsule is developed using gelatin modified with hydroxypyridinones (Gel-HOPO), a catechol analogue with oxidant-resistant propert...
Comparative photodynamic inactivation of antibiotic resistant bacteria by first...
Costa, Dora C. S.; Gomes, Maria Clara; Faustino, Maria A. F.; Neves, Maria G. P. M. S.; Cunha, Ângela; Cavaleiro, José A. S.; Almeida, AdelaidePhotodynamic inactivation (PDI) is an efficient approach against a wide range of microorganisms and can be viewed as an alternative for the treatment of microbial infections. In this work we synthesized "first" and "second" generation photosensitizers (PSs), the tetra-cationic porphyrin and the new penta-cationic chlorin , respectively, and evaluated their efficiency against two antibiotic resistant bacterial s...