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Influence of lysosomal protease sensitivity in the immunogenicity of the antitu...

Rodrigues, Mariane A.D.; Pimenta, Marcela V.; Costa, Iris M.; Zenatti, Priscila P.; Migita, Natacha A.; Yunes, José A.; Rangel-Yagui, Carlota O.

Made available in DSpace on 2021-06-25T10:35:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-12-01; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Ministério da Ciência, Tecnologia, Inovações e Comunicações; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); L-asparaginase (ASNase) from Escherichia ...

Date: 2021   |   Origin: Oasisbr

Heterologous expression and purification of active L-asparaginase I of Saccharo...

Santos, João H. P. M.; Costa, Iris M.; Molino, João V. D.; Leite, Mariana S. M.; Pimenta, Marcela V.; Coutinho, João A. P.; Pessoa, Adalberto

l-asparaginase (ASNase) is a biopharmaceutical widely used to treat child leukemia. However, it presents some side effects, and in order to provide an alternative biopharmaceutical, in this work, the genes encoding ASNase from Saccharomyces cerevisiae (Sc_ASNaseI and Sc_ASNaseII) were cloned in the prokaryotic expression system Escherichia coli. In the 93 different expression conditions tested, the Sc_ASNaseII ...


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