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Antigenic peptides capable of inducing specific antibodies for detection of the...
Paro, Marina de Oliveira; Ferreira, Cyntia Silva; Vieira, Fernanda Silva; Santana, Marcos Aurélio de; Borges, William de CastroVonWillebrand disease (VWD) is an inherited hemorrhagic disorder promoted by either quantitative or qualitative defects of the von Willebrand factor (VWF). The disease represents the most common human coagulopathy afflicting 1.3% of the population. Qualitative defects are subdivided into four subtypes and classified according to the molecular dysfunction of the VWF. The differential diagnosis of the VWD is a di...
A novel and efficient and low-cost methodology for purification of Macrotyloma ...
Santana, Marcos Aurélio de; Santos, Alexandre Martins Costa; Oliveira, Marcelo Eurípedes; Oliveira, Jamil Silvano de; Babá, Élio HideoThe N-acetyl-galactosamine specific lectin fromMacrotyloma axillare seeds (LMA)was purified by precipitation and ion exchange chromatography. The LMA 0.2 mol L−1 fraction showed hemagglutinating activity on erythrocytes A1. The results for molecular mass determinations were about 28 kDa. The LMA pHdependent assays showed best hemagglutinating activity at pH 6.0–8.0; being decreased at acidic/alkaline conditions...
Isolamento, propriedades bioquímicas e estudos biológicos da lectina de semente...
Santana, Marcos Aurélio deDuas frações de uma lectina específica para 2-N-acetil-galactosamina foram isoladas de sementes da Macrotyloma axillare (LMA) através de uma metodologia alternativa a cromatografia de afinidade. As frações ativas foram purificadas por precipitação térmica do extrato bruto a 90°C por 30 min; precipitação com etanol a frio na concentração de 20-60%, seguido de cromatografia de troca iônica em pH 8,3 procedendo-se...