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Bioremediation of drainwater from soilless cultivation by Tetradesmus obliquus ...

Maia, Inês Beatriz; Pinto, Bruno; Carneiro, Mariana; Konucu, Merve; Schulze, Peter S.C.; Santos, Tamara; Rodrigues, Alexandre M.C.

To meet the demands of the growing population, agricultural practices have been increasing and putting a strain on land and freshwater usage. Soilless agriculture has emerged as a more sustainable practice to mitigate this issue but still generates nutrient-rich drainwater that can harm the environment if not properly managed. In this context, this study explores a circular economy approach to reuse the drainwa...


Glyphosate: a terrestrial threat to marine plants? a study on the seagrass zost...

Deguette, Alizé; Pes, Katia; Vasconcellos, Bernard; Costa, Monya; Silva, João; Barrote, Isabel

Glyphosate-based herbicides (GBHs) are extensively used worldwide, raising concerns about their potential effect on non-target aquatic ecosystems. This study investigated the short-term physiological effects of a commercially available GBH on the seagrass Zostera marina under controlled mesocosm conditions. Z. marina individuals were exposed to three concentrations of glyphosate (0.165, 51, and 5100 mg L−1 ) fo...


Heterotrophic cultivation of Chlorella vulgaris yellow mutant on sidestreams: M...

Trovão, Mafalda; Barros, Ana; Machado, Adriana; Reis, Ana; Pedroso, Humberto; Espírito Santo, Gonçalo; Correia, Nádia; Costa, Monya; Ferreira, Sara

Microalgal protein is a promising feedstock to complement and/or replace other protein sources. Besides requiring less land and water usage, microalgae production is a more sustainable process, especially if industrial sidestreams are used as nutrient sources. Additionally, the heterotrophic cultivation of microalgae, such as Chlorella vulgaris, enables the achievement of much higher biomass productivity and lo...


Oxyfluorfen: a novel metabolic inhibitor to select microalgal chlorophyll-defic...

Trovão dos Santos, Mafalda; Cardoso, Lucas; Schüler, Lisa; Machado, Adriana; Santo, Gonçalo Espírito; Pedroso, Humberto; Reis, Ana; Barros, Ana

Nowadays, there is an increasing demand for novel feedstocks and alternative protein sources to meet global needs. Because of their rich nutritional profiles and high protein contents, microalgae-based food products and supplements are being developed. Nonetheless, these products present organoleptic characteristics such as taste, smell and colour that are often considered unpleasant by human and animal consume...


Isolation and selection of protein-rich mutants of chlorella vulgaris by fluore...

Trovão dos Santos, Mafalda; Schüler, Lisa; Pedroso, Humberto; Reis, Ana; Santo, Gonçalo Espírito; Barros, Ana; Correia, Nádia; Ribeiro, Joana

Microalgae are a promising feedstock with proven biostimulant activity that is enhanced by their biochemical components (e.g., amino acids and phytohormones), which turns them into an appealing feedstock to reduce the use of fertilisers in agriculture and improve crop productivity and resilience. Thus, this work aimed to isolate protein-rich microalgal mutants with increased biostimulant activity. Random mutage...


Comparison of different pretreatment processes envisaging the potential use of ...

Marques, Fabiana; Pereira, Francisco; Machado, Luís; Martins, Joana T.; Pereira, Ricardo N.; Costa, Monya; Genisheva, Zlatina; Pereira, Hugo

A significant fraction of the food produced worldwide is currently lost or wasted throughout the supply chain, squandering natural and economic resources. Food waste valorization will be an important necessity in the coming years. This work investigates the ability of food waste to serve as a viable nutritional substrate for the heterotrophic growth of Chlorella vulgaris. The impact of different pretreatments o...


Heterotrophic and photoautotrophic media optimization using response surface me...

Correia, Nádia; Pereira, Hugo; Schulze, Peter S.C.; Costa, Monya; Santo, Gonçalo E.; Guerra, Inês; Trovão dos Santos, Mafalda; Barros, Ana

The nutritional requirements of novel microalgal strains are key for their effective cultivation and metabolite content. Therefore, the optimization of heterotrophic and photoautotrophic culture media is crucial for novel Chlorococcum amblystomatis growth. Heterotrophic and photoautotrophic biomass samples were characterized to identify the differences between their heterotrophic and photoautotrophic biomass co...


Daily regulation of key metabolic pathways in two seagrasses under natural ligh...

Ruocco, Miriam; Barrote, Isabel; Hofman, Jan Dirk; Pes, Katia; Costa, Monya; Procaccini, Gabriele; Silva, João; Dattolo, Emanuela

The circadian clock is an endogenous time-keeping mechanism that enables organisms to adapt to external environmental cycles. It produces rhythms of plant metabolism and physiology, and interacts with signaling pathways controlling daily and seasonal environmental responses through gene expression regulation. Downstream metabolic outputs, such as photosynthesis and sugar metabolism, besides being affected by th...


Heatwave effects on the photosynthesis and antioxidant activity of the seagrass...

Costa, Monya; Silva, João; Barrote, Isabel; Santos, Rui

Global climate change, specifically the intensification of marine heatwaves, affect seagrasses. In the Ria Formosa, saturating light intensities may aggravate heatwave effects on seagrasses, particularly during low spring tides. However, the photophysiological and antioxidant responses of seagrasses to such extreme events are poorly known. Here, we evaluated the responses of <i>Cymodocea nodosa</i> exposed at 2...


A review of common parameters and descriptors used in studies of the impacts of...

Costa, Giulia Burle; Koerich, Gabrielle; Ramos, Bruna de; Ramlov, Fernanda; Martínez-Crego, Begoña; Costa, Monya; Jesus, Dora; Santos, Rui

This study presents a systematic review to assess the main similarities and gaps in efforts to evaluate the impacts of heavy metals on benthic marine seaweeds. A total of 91 studies were compiled, the main parameters (abiotic, biological, ecotoxicological, and heavy metals) and descriptors of which were evaluated by quantitative and qualitative analyses. Our results indicate the importance of diversifying searc...


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