48 documents found, page 1 of 5
Assessment of the Genotoxic Hazard of Estuarine Sediments Using an Integrative ...
Pinto, Miguel; Sacadura, Joana; Costa, Pedro M.; Caeiro, Sandra; Louro, Henriqueta; Silva, Maria J.Under the influence of multiple anthropogenic pressures, from industrial to agricultural activities, estuaries have long been regarded as particularly sensitive ecosystems to contamination. The present study aimed at investigating the genotoxic potential of a contaminated sediment sample from an urban and industrial area of the Sado Estuary, by combining the analysis of multiple endpoints in the <jats:italic>La...
Assessment of the genotoxic hazard of estuarine sediments using an integrative ...
Pinto, Miguel; Sacadura, Joana; Costa, Pedro M.; Caeiro, Sandra; Louro, Henriqueta; Silva, Maria J.Under the influence of multiple anthropogenic pressures, from industrial to agricultural activities, estuaries have long been regarded as particularly sensitive ecosystems to contamination. The present study aimed at investigating the genotoxic potential of a contaminated sediment sample from an urban and industrial area of the Sado Estuary, by combining the analysis of multiple endpoints in the LacZ plasmid-ba...
A comparative analysis of the venom system between two morphotypes of the sea a...
Alcaide, Maria; Cabral, Inês Moutinho; Carvalho, Lara; Mendes, Vera M.; Matos, António P. Alves de; Manadas, Bruno; Saúde, LeonorThe current study investigates the venom-delivery system of green and red morphotypes of the sea anemone Actinia equina to disclose its potential as a source of bioactive compounds. We compared the two morphotypes using electron and optical microscopy, proteomics, and toxicity assessment on zebrafish embryos. Specialized venom-injecting cells (nematocysts) are equally distributed and found in the tentacles of b...
Transcriptome profiling of the posterior salivary glands of the cuttlefish Sepi...
Gonçalves, Cátia; Cabral, Inês Moutinho; Matos, António P. Alves de; Grosso, Ana R.; Costa, Pedro M.Cephalopods like octopuses and cuttlefishes are known to secrete a ‘toxic saliva’ to inject into their prey, especially crustaceans since the XIX century. However, only in the mid-XX century were the first coleoid-specific toxins successfully isolated. Motivated by the growing interest on the global ocean as an almost inexhaustible source of novel bioactive compounds, we used RNA-Seq – based transcriptomics and...
Endogenous Fluorescent Proteins in the Mucus of an Intertidal Polychaeta: Clues...
Rodrigo, Ana P.; Lopes, Ana; Pereira, Ricardo; Anjo, Sandra I.; Manadas, Bruno; Grosso, Ana R.; Baptista, Pedro V.; Fernandes, Alexandra R.The vast ocean holds many unexplored organisms with unique adaptive features that enable them to thrive in their environment. The secretion of fluorescent proteins is one of them, with reports on the presence of such compounds in marine annelids being scarce. The intertidal Eulalia sp. is an example. The worm secretes copious amounts of mucus, that when purified and concentrated extracts, yield strong fluoresce...
Differential effects of food restriction and warming in the two-spotted goby: I...
Lopes, Ana F.; Murdoch, Robyn; Cardoso, Sara D.; Madeira, Carolina; Costa, Pedro M.; Félix, Ana Sofia; Oliveira, Rui Filipe; Bandarra, NarcisaClimate change is a growing threat to marine organisms and ecosystems, and it is already modifying ocean properties by, for example, increasing temperature and decreasing pH. Increasing water temperature may also lead to an impairment of primary productivity and an overall depletion of available zooplankton. Understanding how the crossover between warming and zooplankton availability impacts fish populations ha...
Differential effects of food restriction and warming in the two-spotted goby: i...
Lopes, Ana F.; Murdoch, Robyn; Martins-Cardoso, Sara; Madeira, Carolina; Costa, Pedro M.; Félix, Ana S.; Oliveira, Rui F.; Bandarra, Narcisa M.Climate change is a growing threat to marine organisms and ecosystems, and it is already modifying ocean properties by, for example, increasing temperature and decreasing pH. Increasing water temperature may also lead to an impairment of primary productivity and an overall depletion of available zooplankton. Understanding how the crossover between warming and zooplankton availability impacts fish populations ha...
Specific Antiproliferative Properties of Proteinaceous Toxin Secretions from th...
Rodrigo, Ana P.; Mendes, Vera M.; Manadas, Bruno; Grosso, Ana R.; Alves de Matos, António P.; Baptista, Pedro V.; Costa, Pedro M.As Yondelis joins the ranks of approved anti-cancer drugs, the benefit from exploring the oceans' biodiversity becomes clear. From marine toxins, relevant bioproducts can be obtained due to their potential to interfere with specific pathways. We explored the cytotoxicity of toxin-bearing secretions of the polychaete Eulalia onto a battery of normal and cancer human cell lines and discovered that the cocktail of...
Different sensitivity to heatwaves across the life cycle of fish reflects pheno...
Madeira, Diana; Madeira, Carolina; Costa, Pedro M.; Vinagre, Catarina; Portner, Hans-Otto; Diniz, Mario S.Predicting responses of marine organisms to global change requires eco-physiological assessments across the complex life cycles of species. Here, we experimentally tested the vulnerability of a demersal temperate fish (Sparus aurata) to long-lasting heatwaves, on larval , juvenile and adult life-stages. Fish were exposed to simulated coastal (18 degrees C), estuarine (24 degrees C) summer temperatures, and heat...
Different sensitivity to heatwaves across the life cycle of fish reflects pheno...
Madeira, Diana; Madeira, Carolina; Costa, Pedro M.; Vinagre, Catarina; Pörtner, Hans-Otto; Diniz, Mário S.Predicting responses of marine organisms to global change requires eco-physiological assessments across the complex life cycles of species. Here, we experimentally tested the vulnerability of a demersal temperate fish (Sparus aurata) to long-lasting heatwaves, on larval, juvenile and adult life-stages. Fish were exposed to simulated coastal (18 °C), estuarine (24 °C) summer temperatures, and heatwave conditions...