10 documents found, page 1 of 1
Diversidade da microbiota de fungos da própolis in natura
Costa, Rodrigo Arthur Fonseca; Bispo, Aline Simões da Rocha; Choupina, Altino; Evangelista-Barreto, Norma Suely; Carvalho, Carlos Alfredo Lopes deO presente estudo teve por objetivo realizar a identificação dos fungos encontrados na própolis produzido por Apis mellifera L. da Baía do Iguape, Brasil. Para tanto, foram utilizadas técnicas morfológicas, bioquímicas e moleculares, sendo averiguado o perfil de restrição gerado por espaçador interno transcrito (ITS1 e ITS4). O tamanho dos produtos de PCR foi analisado quanto ao perfil de restrição obtidos com ...
Cloning, characterization, in vitro and in planta expression of a necrosis‑indu...
Martins, Ivone; Meirinho, Sofia G.; Costa, Rodrigo Arthur Fonseca; Cravador, Alfredo; Choupina, AltinoThe soil-borne oomycete Phytophthora cinnamomi is a highly destructive Phytophthora species associated with the decline of forest. This pathogen secretes a novel class of necrosis-inducing proteins known as Nep1-like proteins (NLPs). In this work, we report the sequencing and molecular characterization of one of these proteins, more specifically the necrosis-inducing Phytophthora protein 1 (NPP1). The ORF of th...
Cloning and expression analysis of an endo‑1,3‑β‑d‑glucosidase from Phytophthor...
Costa, Rodrigo Arthur Fonseca; Choupina, Altino; Dominguez, ÁngelPhytophthora is considered one of the most destructive genus for many agricultural plant species worldwide, with a strong environmental and economic impact. Phytophthora cinnamomi is a highly aggressive Phytophthora species associated with the forest decline and responsible for the ink disease in chestnut trees (Castanea sativa Miller), a culture which is extremely important in Europe. This pathogenicity occurs...
Silencing the gip gene of Phytophthora cinnamomi by iRNA and subcellular locali...
Chahed, Abdessalem; Chatti, Noureddine; Costa, Rodrigo Arthur Fonseca; Sousa, Maria João; Choupina, AltinoInk Disease is considered one of the most important causes of the decline of chestnut orchards. The break-in yield of Castanea sativa Mill is mainly caused by Phytophthora cinnamomi one of the most aggressive and widespread plant pathogen causing enormous economic losses and up to now, no efficient treatments are available to fight these pathogens. Because of the importance of chestnut at economical and ecologi...
Knockdown of Phytophthora cinnamomi gip gene by iRNA
Chahed, Abdessalem; Costa, Rodrigo Arthur Fonseca; Sousa, Maria João; Choupina, AltinoInk Disease is considered one of the most important causes of the decline of chestnut orchards. The break in yield of Castanea sativa Mill is mainly caused by Phytophthora cinnamomi one of the most aggressive and widespread plant pathogen causing enormous economic losses and up to now no efficient treatments are available to fight these pathogens. Because of the importance of chestnut at economical and ecologic...
Determination of the subcellular localisation of GIP and NPP1 proteins in Phyto...
Chahed, Abdessalem; Chatti, Noureddine; Costa, Rodrigo Arthur Fonseca; Sousa, Maria João; Choupina, AltinoP. cinnamomi is considered one of the world's worst invasive pathogens with a serious threat to a wide range of plant species throughout the world. These species cause several billions of dollars of damage on crop, ornamental, and native plants and so far no efficient solutions have been found to control the disease. A notable characteristic of the interaction between plants and their microbial pathogens is the...
Silencing the gip gene of Phytophthora cinnamomi by iRNA and subcellular locali...
Chahed, Abdessalem; Chatti, Noureddine; Costa, Rodrigo Arthur Fonseca; Sousa, Maria João; Choupina, AltinoInk Disease is considered one of the most important causes of the decline of chestnut orchards. The break in yield of Castanea sativa Mill is mainly caused by Phytophthora cinnamomi one of the most aggressive and widespread plant pathogen causing enormous economic losses and up to now no efficient treatments are available to fight these pathogens. Because of the importance of chestnut at economical and ecologic...
Transglutaminases: recent achievements and new sources
Martins, Ivone; Matos, Mauro; Costa, Rodrigo Arthur Fonseca; Silva, Fátima H.; Pascoal, Ananias; Estevinho, Leticia M.; Choupina, AltinoTransglutaminases are a family of enzymes (EC 2.3.2.13), widely distributed in various organs, tissues, and body fluids, that catalyze the formation of a covalent bond between a free amine group and the γ-carboxamide group of protein or peptide-bound glutamine. Besides forming these bonds, that exhibit high resistance to proteolytic degradation, transglutaminases also form extensively cross-linked, generally in...
Sequence analysis and expression of genes involved cytoskeleton and infection m...
Choupina, Altino; Estevinho, Leticia M.; Costa, Rodrigo Arthur Fonseca; Dominguez, ÁngelOomycetes from the genus Phytophthora are plant pathogens that are devastating for agriculture and natural ecosystems. The biggest productivity and yield break occurs due to the ink disease; caused by Phytophthora cinnamomi, which is one of the most widely distributed Phytophthora species, with nearly 1 000 host species. The knowledge about the molecular mechanisms responsible for its pathogenicity is an import...
Enzimas β-Glucanases: aplicações da hidrólise de polissacarídeos /β-Glucanases ...
Costa, Rodrigo Arthur Fonseca; Choupina, AltinoThe enzymatic degradation of polysaccharides is accomplished through the action of several hydrolases with different specificities and way of action. For this reason, there is now a growing interest in the study of these enzymes related to its potential industry. The β-glucanases enzymes belong to this group of hydrolases because of its ability to hydrolyze glycosidiclinkages β-D-glucosides 1,3-β-glucans and 1,...