12 documents found, page 1 of 2
Use of iRNA in the post-transcriptional gene silencing of necrosis-inducing Phy...
Pascoal‑Ferreira, Patrick; Chahed, Abdessalem; Costa, Rodrigo Arthur Fonseca; Branco, Iuliia; Choupina, AltinoPhytophthora cinnamomi is an Oomycetes associated with soil, this Oomycete is one of the most destructive species of Phytophthora, being responsible for the decline of more than 5000 ornamental, forest, or fruit plants. It can secrete a class of protein NPP1 (Phytophthora necrosis inducing protein 1), responsible for inducing necrosis in leaves and roots of plants, leading to their death. Objective This work wi...
Post-transcriptional gene silencing of glucanase inhibitor protein in Phytophth...
Ferreira, Patrick; Chahed, Abdessalem; Estevinho, Leticia M.; Seixas, Natália da Assunção Lisboa; Costa, Rodrigo Arthur Fonseca; Choupina, AltinoInk disease is considered one of the most significant causes contributing to the decline of chestnut orchards. The reduced yield of Castanea sativa Mill can be attributed to two main species: Phytophthora cinnamomi and Phytophthora cambivora, with the first being the main pathogen responsible for ink disease in Portugal. P. cinnamomi is a highly aggressive and widely distributed plant pathogen, capable of infec...
Diversidade da microbiota de fungos da própolis in natura
Costa, Rodrigo Arthur Fonseca; Bispo, Aline Simões da Rocha; Choupina, Altino; Evangelista-Barreto, Norma Suely; Carvalho, Carlos Alfredo Lopes deO presente estudo teve por objetivo realizar a identificação dos fungos encontrados na própolis produzido por Apis mellifera L. da Baía do Iguape, Brasil. Para tanto, foram utilizadas técnicas morfológicas, bioquímicas e moleculares, sendo averiguado o perfil de restrição gerado por espaçador interno transcrito (ITS1 e ITS4). O tamanho dos produtos de PCR foi analisado quanto ao perfil de restrição obtidos com ...
Cloning and expression analysis of an endo‑1,3‑β‑d‑glucosidase from Phytophthor...
Costa, Rodrigo Arthur Fonseca; Choupina, Altino; Dominguez, ÁngelPhytophthora is considered one of the most destructive genus for many agricultural plant species worldwide, with a strong environmental and economic impact. Phytophthora cinnamomi is a highly aggressive Phytophthora species associated with the forest decline and responsible for the ink disease in chestnut trees (Castanea sativa Miller), a culture which is extremely important in Europe. This pathogenicity occurs...
Cloning, characterization, in vitro and in planta expression of a necrosis‑indu...
Martins, Ivone; Meirinho, Sofia G.; Costa, Rodrigo Arthur Fonseca; Cravador, Alfredo; Choupina, AltinoThe soil-borne oomycete Phytophthora cinnamomi is a highly destructive Phytophthora species associated with the decline of forest. This pathogen secretes a novel class of necrosis-inducing proteins known as Nep1-like proteins (NLPs). In this work, we report the sequencing and molecular characterization of one of these proteins, more specifically the necrosis-inducing Phytophthora protein 1 (NPP1). The ORF of th...
Determination of the subcellular localisation of GIP and NPP1 proteins in Phyto...
Chahed, Abdessalem; Chatti, Noureddine; Costa, Rodrigo Arthur Fonseca; Sousa, Maria João; Choupina, AltinoP. cinnamomi is considered one of the world's worst invasive pathogens with a serious threat to a wide range of plant species throughout the world. These species cause several billions of dollars of damage on crop, ornamental, and native plants and so far no efficient solutions have been found to control the disease. A notable characteristic of the interaction between plants and their microbial pathogens is the...
Silencing the gip gene of Phytophthora cinnamomi by iRNA and subcellular locali...
Chahed, Abdessalem; Chatti, Noureddine; Costa, Rodrigo Arthur Fonseca; Sousa, Maria João; Choupina, AltinoInk Disease is considered one of the most important causes of the decline of chestnut orchards. The break-in yield of Castanea sativa Mill is mainly caused by Phytophthora cinnamomi one of the most aggressive and widespread plant pathogen causing enormous economic losses and up to now, no efficient treatments are available to fight these pathogens. Because of the importance of chestnut at economical and ecologi...
Knockdown of Phytophthora cinnamomi gip gene by iRNA
Chahed, Abdessalem; Costa, Rodrigo Arthur Fonseca; Sousa, Maria João; Choupina, AltinoInk Disease is considered one of the most important causes of the decline of chestnut orchards. The break in yield of Castanea sativa Mill is mainly caused by Phytophthora cinnamomi one of the most aggressive and widespread plant pathogen causing enormous economic losses and up to now no efficient treatments are available to fight these pathogens. Because of the importance of chestnut at economical and ecologic...
Silencing the gip gene of Phytophthora cinnamomi by iRNA and subcellular locali...
Chahed, Abdessalem; Chatti, Noureddine; Costa, Rodrigo Arthur Fonseca; Sousa, Maria João; Choupina, AltinoInk Disease is considered one of the most important causes of the decline of chestnut orchards. The break in yield of Castanea sativa Mill is mainly caused by Phytophthora cinnamomi one of the most aggressive and widespread plant pathogen causing enormous economic losses and up to now no efficient treatments are available to fight these pathogens. Because of the importance of chestnut at economical and ecologic...
Transglutaminases: recent achievements and new sources
Martins, Ivone; Matos, Mauro; Costa, Rodrigo Arthur Fonseca; Silva, Fátima H.; Pascoal, Ananias; Estevinho, Leticia M.; Choupina, AltinoTransglutaminases are a family of enzymes (EC 2.3.2.13), widely distributed in various organs, tissues, and body fluids, that catalyze the formation of a covalent bond between a free amine group and the γ-carboxamide group of protein or peptide-bound glutamine. Besides forming these bonds, that exhibit high resistance to proteolytic degradation, transglutaminases also form extensively cross-linked, generally in...