7 documents found, page 1 of 1
Effect of hyaluronan molecular weight on the stability and biofunctionality of ...
Albuquerque, A. V.; Soares da Costa, D.; Correia, Cátia Sofia Palma; Reis, R. L.; Alves, N. M.; Costa, Rui Filipe Ramos; Pashkuleva, I.Nervous system disorders are characterized by a progressive loss of function and structure of neurons that ultimately leads to a decline in cognitive and motor functions. In this study, we used interfacial polyelectrolyte complexation (IPC) to produce fibers for neural tissue regeneration. IPC is a processing method that allows spinning of sensitive biopolymers. The rate of spinning and the properties of the us...
Projeto e automatização de uma bancada de testes em válvulas esféricas
Costa, Rui Filipe RamosEste relatório detalha o desenvolvimento de uma bancada de testes em válvulas, estruturada em dois módulos principais: o módulo de controlo e o módulo de suporte e fixação. A necessidade deste projeto emergiu da análise crítica das instalações de certificação existentes na empresa, que revelou tanto as vantagens quanto as limitações do sistema em uso, fornecendo diretrizes claras para a possível reutilização, o...
A label-free multitechnique approach to characterize the interaction of bioacti...
Fernandes, Eduarda; Costa, Rui Filipe Ramos; Machado, Raúl; Reis, R. L.; Pashkuleva, I.; Lúcio, MarleneExtensive research has been conducted on biomimetic interfaces mimicking the complex and diverse microenvironment of cell membranes to gain insights into bioactive compound interactions and membrane biophysics modulation. The present study proposes an innovative approach that combines five prospective label-free methodologies (derivative spectroscopy, synchrotron small- and wide-angle X-Ray scattering, attenuat...
Layer-by-layer coated calcium carbonate nanoparticles for targeting breast canc...
Bastos, F. R.; Soares da Costa, Diana; Reis, R. L.; Alves, N. M.; Pashkuleva, I.; Costa, Rui Filipe RamosBreast cancer is resistant to conventional treatments due to the specific tumour microenvironment, the associated acidic pH and the overexpression of receptors that enhance cells tumorigenicity. Herein, we optimized the synthesis of acidic resorbable calcium carbonate (CaCO3) nanoparticles and the encapsulation of a low molecular weight model molecule (Rhodamine). The addition of ethylene glycol during the synt...
Glycosaminoglycans as polyelectrolytes: implications in bioactivity and assembl...
Costa, Rui Filipe Ramos; Reis, R. L.; Pashkuleva, I.The innate negative charge of glycosaminoglycans (GAG) is in the origin of their bioactivity: it drives their spontaneous complexation with positively charged biomolecules and ions, regulating homeostatic functions by protein stabilization, protection and activation. Copycatting these interactions enables different supramolecular approaches towards the assembly of biofunctional devices. Such approaches allow pr...
Mineralization of layer-by-layer ultrathin films containing microfluidic-produc...
Rial, R.; Costa, Rui Filipe Ramos; Reis, R. L.; Liu, Z.; Pashkuleva, I.; Ruso, J. M.We describe the assembly of layer-by-layer (LbL) ultrathin films containing bioactive hydroxyapatite (HAp) rod-shaped nanoparticles with mineralizing capacity. Monodisperse 96 nm long and 9 nm wide HAp nanorods with a surface charge of â 14 mV were produced with a microfluidic system. The negatively charged HAp nanorods were assembled with the polycation poly-L-lysine (PLL) in LbL fashion. The successful deposi...
Oxidized cashew gum scaffolds for tissue engineering
Maciel, Jeanny S.; Azevedo, Sara; Correia, Clara R.; Costa, Ana M. S.; Costa, Rui Filipe Ramos; Magalhães, Francisco A.; Monteiro, Aliny Abreu deIn the last few years, several strategies have been proposed to fabricate scaffolds for tissue engineering (TE) applications; however, they are based on harsh and timeâ consuming techniques. The choice for natural polymers such as cashew gum (CG) allows to circumvent the demands of biocompatibility and degradability of TE systems. In this work, CG, a polysaccharide derived from Anacardium occidentale trees, is ...