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Development of a phage-based lab-on-chip for the multiplex detection of bloodst...
Costa, Susana Patrícia ChavesBloodstream infections (BSIs) are characterized by high incidence and mortality and thus can greatly impact healthcare systems. The prompt administration of antibiotics along with their de-escalation as early as possible are key elements for BSIs effective control. One of the main constraints that limit these actions is the difficulty in detecting the BSIs’ causative agents. The current diagnostic approaches su...
A microfluidic platform combined with bacteriophage receptor binding proteins f...
Costa, Susana Patrícia Chaves; Caneira, Catarina R. F.; Chu, Virginia; Freitas, Paulo P.; Conde, João P.Bloodstream infections (BSIs) are triggered by the existence of pathogens in blood and are considered a major health burden worldwide, especially when they result in sepsis and septic shock. Common diagnostic methods are time-consuming, present low specificity, or suffer from interference of blood components, which hampers a timely and effective treatment of BSIs. In this work, a novel microfluidic assay was de...
Potential of bacteriophage proteins as recognition molecules for pathogen detec...
Costa, Susana Patrícia Chaves; Nogueira, Catarina L.; Cunha, Alexandra Maria Pinto; Lisac, Ana; Carvalho, Carla Alexandra Oliveira Cunha MedeirosBacterial pathogens are leading causes of infections with high mortality worldwide having a great impact on healthcare systems and the food industry. Gold standard methods for bacterial detection mainly rely on culture-based technologies and biochemical tests which are laborious and time-consuming. Regardless of several developments in existing methods, the goal of achieving high sensitivity and specificity, as...
Burden of bacterial bloodstream infections and recent advances for diagnosis
Costa, Susana Patrícia Chaves; Carvalho, Carla Alexandra Oliveira Cunha MedeirosBloodstream infections (BSIs) and subsequent organ dysfunction (sepsis and septic shock) are conditions that rank among the top reasons for human mortality and have a great impact on healthcare systems. Their treatment mainly relies on the administration of broad-spectrum antimicrobials since the standard blood culture-based diagnostic methods remain time-consuming for the pathogen's identification. Consequentl...
A phage receptor-binding protein as a promising tool for the detection of Esche...
Costa, Susana Patrícia Chaves; Cunha, Alexandra Maria Pinto; Freitas, Paulo P.; Carvalho, Carla Alexandra Oliveira Cunha MedeirosEscherichia coli is a problematic pathogen that causes life-threatening diseases, being a frequent causative agent of several nosocomial infections such as urinary tract and bloodstream infections. Proper and rapid bacterial identification is critical for allowing prompt and targeted antimicrobial therapy. (Bacterio)phage receptor-binding proteins (RBPs) display high specificity for bacterial surface epitopes a...
Bacteriophage-receptor binding proteins for multiplex detection of Staphylococc...
Santos, Sílvio Roberto Branco; Cunha, Alexandra; Macedo, M.; Nogueira, Catarina; Brandão, Ana Catarina; Costa, Susana Patrícia ChavesHealth care-associated infections (HCAIs) affect hundreds of millions of patients, representing a significant burden for public health. They are usually associated to multidrug resistant bacteria, which increases their incidence and severity. Bloodstream infections (BSIs) are among the most frequent and life-threatening HCAIs, with Enterococcus and Staphylococcus among the most common isolated pathogens. The co...
Development of a phage-based lab-on-chip for the detection of foodborne pathogens
Costa, Susana Patrícia ChavesFoodborne diseases are caused by the ingestion of contaminated food or water, representing a worldwide problem. Campylobacter and Staphylococcus are important pathogens to control, since they are frequent causes of foodborne illness and represent a challenge for the food industry. The microbiological techniques remain the gold standard for detection of pathogens but are time consuming and extremely laborious. O...