8 documents found, page 1 of 1
Exploring quercetin's potential to counteract intestinal pro-inflammatory effec...
Sousa, Adelaide; Azevedo, Rui; Costa, Vera M.; Oliveira, Sara; Viana, Sofia; Reis, Flávio; Almeida, Agostinho; Matafome, Paulo; Dias-Pereira, PatríciaBackground: Silver nanoparticles (AgNP) elicit an intestinal response characterized by vascular and cellular changes, alongside the release of cytokines linked to the activation of the nuclear factor-кB (NF-кB) pathway. Consequently, a search was conducted for a compound capable of counteracting the primary pro-inflammatory effects induced by these nanoparticles [1]. Given the reported anti-inflammatory propert...
Anticancer drugs and their impact on chemobrain development: an in vitro invest...
Barbosa-Azevedo, Maria; Cardoso, Maria; Carvalho, Félix; Costa, Vera M.Background: Cancer incidence has been increasing worldwide, over the past few years. “Chemobrain” refers to alterations in cognitive function after cancer treatment, including memory deficits and reduced attention capacity [1]. The blood-brain barrier restricts the entry of certain anticancer drugs into the brain. However, "chemobrain" can also arise from factors extending far beyond direct drug exposure to the...
Methods for the analysis of transcriptome dynamics
Rodrigues, Daniela F.; Costa, Vera M.; Silvestre, Ricardo Jorge Leal; Bastos, Maria L.; Carvalho, FélixThe transcriptome is the complete set of transcripts in a cell or tissue and includes ribosomal RNA (rRNA), messenger RNA (mRNA), transfer RNA (tRNA), and regulatory noncoding RNA. At steady-state, the transcriptome results from a compensatory variation of the transcription and decay rate to maintain the RNA concentration constant. RNA transcription constitutes the first stage in gene expression, and thus is a ...
A breakthrough on Amanita phalloides poisoning: an effective antidotal effect b...
Garcia, Juliana; Costa, Vera M.; Carvalho, Alexandra T.P.; Silvestre, Ricardo; Duarte, José A.; Dourado, Daniel F.A.R.; Arbo, Marcelo D.Amanita phalloides is responsible for more than 90 % of mushroom-related fatalities, and no effective antidote is available. α-Amanitin, the main toxin of A. phalloides, inhibits RNA polymerase II (RNAP II), causing hepatic and kidney failure. In silico studies included docking and molecular dynamics simulation coupled to molecular mechanics with generalized Born and surface area method energy decomposition on ...
Co-ingestion of amatoxins and isoxazoles-containing mushrooms and successful tr...
Garcia, Juliana; Costa, Vera M.; Costa, Ana Elisa; Andrade, Sérgio; Carneiro, Ana Cristina; Conceição, Filipe; Paiva, José Artur; Pinho, P. Guedes deMushroom poisonings occur when ingestion of wild mushrooms containing toxins takes place, placing the consumers at life-threatening risk. In the present case report, an unusual multiple poisoning with isoxazoles- and amatoxins-containing mushrooms in a context of altered mental state and poorly controlled hypertension is presented. A 68-year-old female was presented to São João hospital (Portugal) with complain...
Amanita phalloides poisoning: Mechanisms of toxicity and treatment
Garcia, Juliana; Costa, Vera M.; Carvalho, Alexandra T.P.; Baptista, Paula; Pinho, P. Guedes de; Bastos, Maria de Lourdes; Carvalho, FélixAmanita phalloides, also known as 'death cap', is one of the most poisonous mushrooms, being involved in the majority of human fatal cases of mushroom poisoning worldwide. This species contains three main groups of toxins: amatoxins, phallotoxins, and virotoxins. From these, amatoxins, especially α-amanitin, are the main responsible for the toxic effects in humans. It is recognized that α-amanitin inhibits RNA ...
Quantification of alpha-amanitin in biological samples by HPLC using simultaneo...
Garcia, Juliana; Costa, Vera M.; Baptista, Paula; Bastos, Maria de Lourdes; Carvalho, Félixα-Amanitin is a natural bicyclic octapeptide, from the family of amatoxins, present in the deadly mushroom species Amanita phalloides. The toxicological and clinical interests raised by this toxin, require highly sensitive, accurate and reproducible quantification methods for pharmacokinetic studies. In the present work, a high-performance liquid chromatographic (HPLC) method with in-line connected diode-array ...
Cumulative Mitoxantrone-Induced Haematological and Hepatic Adverse Effects in a...
Rossato, Luciana G.; Costa, Vera M.; Dallegrave, Eliane; Arbo, Marcelo; Dinis-Oliveira, Ricardo J.; Santos-Silva, Alice; Duarte, José A.Mitoxantrone (MTX) is an antineoplastic agent that can induce hepato- and haematotoxicity. This work aimed to investigate the occurrence of cumulative early and late MTX-induced hepatic and haematological disturbances in an vivo model. A control group and two groups treated with three cycles of 2.5 mg/kg MTX at days 0, 10 and 20 were formed. One of the treated groups suffered euthanasia on day 22 (MTX22) to eva...