8 documents found, page 1 of 1
The hCOMET project: international database comparison of results with the comet...
Milić, Mirta; Ceppi, Marcello; Bruzzone, Marco; Azqueta, Amaya; Brunborg, Gunnar; Godschalk, Roger; Koppen, Gudrun; Langie, Sabine; Møller, PeterThe alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in the human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - repre...
The hCOMET project: International database comparison of results with the comet...
Milić, Mirta; Ceppi, Marcello; Bruzzone, Marco; Azqueta, Amaya; Brunborg, Gunnar; Godschalk, Roger; Koppen, Gudrun; Langie, Sabine; Møller, PeterThe alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - represent...
Harmonized definition of occupational burnout: a systematic review, semantic an...
Guseva Canu, Irina; Marca, Sandy Carla; Dell'Oro, Francesca; Balázs, Ádám; Bergamaschi, Enrico; Besse, Christine; Bianchi, Renzo; Bislimovska, JovankaObjective: A consensual definition of occupational burnout is currently lacking. We aimed to harmonize the definition of occupational burnout as a health outcome in medical research and reach a consensus on this definition within the Network on the Coordination and Harmonisation of European Occupational Cohorts (OMEGA-NET). Methods: First, we performed a systematic review in MEDLINE, PsycINFO and Embase (Januar...
Oxidative DNA damage among workers exposed to noise - a pilot study on non-audi...
Sousa, Catarina; Carvalhais, Carlos; Neves, Maria Paula; Gaivão, Isabel; Teixeira, João Paulo; Costa Pereira, CristianaObjective: This study was designed to assess the effect of occupational noise exposure in nonauditory systems, particularly the cardiovascular system
Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations f...
Møller, Peter; Azqueta, Amaya; Boutet-Robinet, Elisa; Koppen, Gudrun; Bonassi, Stefano; Milić, Mirta; Gajski, Goran; Costa, SolangeThe comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here...
Optimization of the harvesting and freezing conditions of human cell lines for ...
Bessa, Maria João; Brandão, Fátima; Machado Querido, Micaela; Costa Pereira, Cristiana; Valdiglesias, Vanessa; Laffon, Blanca; Carriere, MarieThe comet assay is a commonly used method for in vitro and in vivo genotoxicity assessment. This versatile assay can be performed in a wide range of tissues and cell types. Although most of the studies use samples immediately processed after collection, frozen biological samples can also be used. The present study aimed to optimize a collection and freezing protocol to minimize the DNA damage associated with th...
Association between Polymorphisms in Antioxidant Genes and Inflammatory Bowel D...
Costa Pereira, Cristiana; Durães, Cecília; Coelho, Rosa; Grácio, Daniela; Silva, Marco; Peixoto, Armando; Lago, Paula; Pereira, Márcia; Catarino, TelmoInflammation is the driving force in inflammatory bowel disease (IBD) and its link to oxidative stress and carcinogenesis has long been accepted. The antioxidant system of the intestinal mucosa in IBD is compromised resulting in increased oxidative injury. This defective antioxidant system may be the result of genetic variants in antioxidant genes, which can represent susceptibility factors for IBD, namely Croh...
Project on genetic and epigenetic effects of maternal environmental exposure to...
Costa Pereira, Cristiana; Costa, CarlaPrenatal tobacco smoke exposure in early pregnancy may have important and lasting effects on DNA methylation and consequently influence gene expression and disease phenotypes across the life course. Given the scarcity of studies regarding the genotoxic potential of tobacco smoke during pregnancy and the lack of information regarding the transplacental transfer of different compounds present in tobacco smoke, th...