7 documents found, page 1 of 1
Vapor phase of white thyme essential oil: effect on Candida albicans and preser...
Fernandes, Liliana Sousa; Silva, Inês da; Araújo, Daniela Eira; Costa, Raquel Pinho; Silva, Sónia Carina; Mira, Nuno Pereira; Costa-de-Oliveira, SofiaThe balance of the vaginal ecosystem results from synergistic interactions established between the different colonizing microorganisms. Lactobacillus spp. stands out as a predominant member, acting a crucial protective role in maintaining vaginal health. As vulvovaginal candidiasis remains prevalent and antifungal resistance grows, there is a pressing need for alternative therapies. However, any development of ...
Development and evaluation of microencapsulated oregano essential oil as an alt...
Fernandes, Liliana Sousa; Barco-Tejada, Ainara; Blázquez, Elena; Araújo, Daniela Eira; Ribeiro, Artur; Silva, Sónia Carina; Cussó, LorenaVulvovaginal candidiasis (VVC) is characterized as a very common fungal infection that significantly affects womens health worldwide. Essential oils (EOs) are currently being evaluated as an alternative therapy. The development of efficient techniques such as micro- or nanoencapsulation for protecting and controlling release is essential to overcome the limitations of EO applications. Therefore, the aim of this...
Oregano essential oil: an effective and non-toxic approach for prevent or treat...
Fernandes, Liliana Sousa; Silva, Inês; Blázquez, Elena; Tejada, Ainara; Ribeiro, Artur; Silva, Sónia Carina; Mira, Nuno; Cussó, LorenaVulvovaginal candidiasis (VVC) is one of the most prevalent vaginal infectious diseases, and the emergence of drug-resistant Candida strains has presented a growing challenge in its treatment. This highlights the urgent need to develop effective and non-toxic alternative treatments. In this context, essential oils (EOs) have emerged as a promising alternative considering low toxicity and high antimicrobial acti...
Effect of vapor-phase oregano essential oil on resistant Candida species biofil...
Fernandes, Liliana Sousa; Costa, Raquel; Silva, Sónia Carina; Henriques, Mariana; Costa-de-Oliveira, Sofia; Rodrigues, M. ElisaThis work presents a new approach to the application of essential oils, exposure to the vapor phase, which can be considered a first approach for the development of a complementary or alternative form of vulvovaginal candidiasis (VVC) treatment. VVC is a significant infection caused by Candida species and remains a common disease that affects millions of women every year. Vulvovaginal candidiasis (VVC) is one o...
Discrimination of clinically relevant Candida species by Fourier-transform infr...
Silva, Sónia Carina; Tobaldini-Valerio, Flávia; Costa-de-Oliveira, Sofia; Henriques, Mariana; Azeredo, Joana; Ferreira, Eugénio C.; Lopes, João AlmeidaAccurate Candida species identification remains a challenge due to their phenotypic and genotypic similarity. Species belonging to the *psilosis group, are even described as phenotypically indistinguishable. Also, most of the genotypic methods commonly used to discriminate these species are laborious and very expensive. In this work we developed a Fourier-transform infrared spectroscopy with attenuated total re...
Determination of chitin content in fungal cell wall: an alternative flow cytome...
Costa-de-Oliveira, Sofia; Silva, Ana P.; Miranda, Isabel M.; Salvador, Alexandre; Azevedo, Maria M.; Munro, Carol A.; Rodrigues, Acácio G.The conventional methods used to evaluate chitin content in fungi, such as biochemical assessment of glucosamine release after acid hydrolysis or epifluorescence microscopy, are low throughput, laborious, time-consuming, and cannot evaluate a large number of cells. We developed a flow cytometric assay, efficient, and fast, based on Calcofluor White staining to measure chitin content in yeast cells. A staining i...
Novel method using a laser scanning cytometer for detection of Mycobacteria in ...
Pina-Vaz, Cidália; Costa-de-Oliveira, Sofia; Rodrigues, Acácio G.; Salvador, AlexandreIn order to evaluate the capacity of laser scanning cytometry (LSC) to detect acid-fast bacilli directly on clinical samples, a comparison between Kinyoun-stained smears analyzed under light microscopy and propidium iodide-auramine-stained smears analyzed by LSC was performed. The results were compared with those for culture on BACTEC MGIT 960. LSC is a new, reliable methodology to detect Mycobacteria.