16 documents found, page 1 of 2
Brazilian isolates of Trypanosoma cruzi from humans and triatomines classified ...
Fernandes, Octávio; Souto, Ricardo Peres; Castro, José A.; Pereira, José Borges; Fernandes, Neide Carrara; Junqueira, Ângela Cristina VeríssimoTraditional molecular and biochemical methods, such as schizodeme analysis, karyotyping, DNA fingerprinting, and enzyme electrophoretic profiles, have shown a large variability among Trypanosoma cruzi isolates. In contrast to those results, polymerase chain reaction (PCR) amplification of sequences from the 24Sα ribosomal RNA gene and from the mini-exon gene nontranscribed spacer indicated a dimorphism among T....
Epidemiology, control and surveillance of Chagas disease - 100 years after its ...
Coura, José Rodrigues; Dias, João Carlos PintoFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Doenças Parasitárias. Rio de Janeiro, RJ, Brasil.; Fundação Oswaldo Cruz. Instituto de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.; Chagas disease originated millions of years ago as an enzootic infection of wild animals and began to be transmitted to humans as an anthropozoonosis when man invaded wild ecotopes. While evidence of human infecti...
The main sceneries of Chagas disease transmission. The vectors, blood and oral ...
Coura, José RodriguesFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Doenças Parasitárias. Rio de Janeiro, RJ, Brasil.; This review deals with transmission of Trypanosoma cruzi by the most important domestic vectors, blood transfusion and oral intake. Among the vectors, Triatoma infestans, Panstrongylus megistus, Rhodnius prolixus, Triatoma dimidiata, Triatoma brasiliensis, Triatoma pseudomaculata, Triatoma sordida, T...
A serological, parasitological and clinical evaluation of untreated Chagas dise...
Assis, Girley Francisco Machado de; Diniz, Glaucia Alessio; Montoya, Roberto Araújo; Dias, João Carlos Pinto; Coura, José RodriguesThe etiological treatment of Chagas disease is recommended for all patients with acute or recent chronic infection, but controversies remain regarding the benefit of chemotherapy and interpretations of the parasitological cure after etiological treatment. This study compares the laboratory and clinical evaluations of Chagas disease patients who were diagnosed 13 years earlier. Fifty-eight Chagas disease patient...
Clinical and laboratory status of patients with chronic Chagas disease living i...
Lana, Marta de; Lopes, Leonardo de Araújo; Martins, Helen Rodrigues; Bahia, Maria Terezinha; Assis, Girley Francisco Machado deTwenty-eight Chagas disease patients (CD), 22 with the indeterminate clinical form (IND) and six with the cardiac or digestive form (CARD/DIG), were treated with benznidazole and underwent clinical and laboratorial analysis before (IND and CARD/DIG) and nine years after [patients after treatment (CDt), patients with the indeterminate clinical form at treatment onset (INDt) and with the cardiac or digestive form...
Evaluation of three different DNA extraction methods from blood samples collect...
Miguel, Renata Bortolasse; Coura, José Rodrigues; Samudio, Franklyn; Suárez-Mutis, Martha CecíliaAsymptomatic Plasmodium infection is a new challenge for public health in the American region. The polymerase chain reaction (PCR) is the best method for diagnosing subpatent parasitemias. In endemic areas, blood collection is hampered by geographical distances and deficient transport and storage conditions of the samples. Because DNA extraction from blood collected on filter paper is an efficient method for mo...
Cross-sectional study defines difference in malaria morbidity in two Yanomami c...
Tosta, Carlos Eduardo; Morgado, Anastácio; Coura, José Rodrigues; Marcano, Teodardo JoséIt is well established that immunity to malaria is short-lived and is maintained by the continuous contact with the parasite. We now show that the stable transmission of malaria in Yanomami Amerindian communities maintains a degree of immunity in the exposed population capable to reduce prevalence and morbidity of malaria. We examined 508 Yanomami Amerindians living along Orinoco (407) and Mucajaí (101) rivers,...
Cross sectional study reveals a high percentage of asymptomatic Plasmodium viva...
Suárez-Mutis, Martha C.; Cuervo, Patricia; Leoratti, Fabiana M.S.; Moraes-Avila, Sandra L.; Ferreira, Antonio Walter; Fernandes, OctavioA parasitological, clinical, serological and molecular cross-sectional study carried out in a highly endemic malaria area of Rio Negro in the Amazon State, Brazil, revealed a high prevalence of asymptomatic Plasmodium vivax infection. A total of 109 persons from 25 families were studied in five villages. Ninety-nine inhabitants (90.8%) had at least one previous episode of malaria. Serology showed 85.7% and 46.9...
The use of the antifungal agent miconazole as an inhibitor of Blastocystis homi...
Gonçalves, Alessandra Queiroga; Viana, João da Costa; Pires, Edna Maria; Bóia, Márcio Neves; Coura, José Rodrigues; Silva, Edward FélixIn regions with high prevalence, Blastocystis hominis is frequently found in association with Entamoeba histolytica/E. dispar in xenic cultures. Its exacerbated growth is often superimposed on the growth of amebas, thus impeding the continuation of the amebas in the culture, within a few generations. The present study reports on the excellent efficacy (100%) of the antifungal agent miconazole in eliminating B. ...
Mass treatment for intestinal helminthisis control in an Amazonian endemic area...
Bóia, Márcio Neves; Carvalho- Costa, Filipe Anibal; Sodré, Fernando Campos; Eyer-Silva, Walter A.; Lamas, Cristiane Cruz; Lyra, Marcelo RosadinskiThe objective of the present study was to estimate the prevalence of soil-transmitted helminthiasis and evaluate the sanitary conditions and the role of a mass treatment campaign for control of these infections in Santa Isabel do Rio Negro. A cross-sectional survey was carried out in 2002, to obtain data related to the sanitary conditions of the population and fecal samples for parasitological examination in 30...