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Epidemiology and clinical presentation of community-acquired Staphylococcus aur...

Garrine, M; Quinto, L; Costa, SS; Messa, A; Massinga, AJ; Vubil, D; Nhampossa, T; Massora, S; Acacio, S; Cossa, A; Sigauque, B; Bassat, Q; Couto, I

Staphylococcus aureus bacteraemia (SAB) is one of the most common bloodstream infections globally. Data on the burden and epidemiology of community-acquired SAB in low-income countries are scarce but needed to defne preventive and management strategies. Blood samples were collected from children<5 years of age with fever or severe disease admitted to the Manhiça District Hospital for bacterial isolation, includ...


Plasmid profiling of Staphylococcus pseudintermedius associated with skin and s...

Morais, C; Costa, SS; Hanke, D.; Krüger-Haker, H.; Santos, A.; Pomba, Constança; Feßler, Andrea T; Schwarz, S,; Couto, I


Assessment of chlorohexidine and benzalkonium chloride activity against Staphyl...

Morais, C; Costa, SS; Pomba, Constança; Couto, I

Introduction: Staphylococcus pseudintermedius is the main bacterial pathogen responsible for skin and soft-tissue infections (SSTIs) in companion animals and is increasingly associated with antimicrobial resistance (AMR). Objective: This study analyzed the effectiveness of the biocides chlorhexidine (CHX) and benzalkonium chloride (BAC) against S. pseudintermedius causing SSTIs in companion animals. Methodology...


CPLP-TB:

Perdigão, João; Silva, Carla; Diniz, Jaciara; Pereira, Catarina; Machado, Diana; Ramos, Jorge; Silva, Hugo; Abilleira , Fernanda; Brum, Clarice

A Tuberculose (TB) permanece um grave problema de saúde pública na Comunidade dos Países de Língua Portuguesa (CPLP). Apesar da ampla variância da incidência da TB nos seus estados-membro e de um fluxo migratório contínuo entre os países que integram este grupo, existe uma enorme lacuna no que diz respeito ao conhecimento da estrutura populacional conjunta do Mycobacterium tuberculosis e circulação de estirpes ...


Complete sequences of two Staphylococcus aureus plasmids carrying genes for res...

Costa, SS; Kadlec, Kristina; Viveiros, M; Melo-Cristino, Josè; Schwarz, Stefan; Couto, I

[P292]; Aim: To gain detailed insight into the genetic organization of two multidrug resistance plasmids, pSM31 and pSM39, from clinical S. aureus isolates collected in a hospital in Lisbon, Portugal, and to identify factors that may explain their selection and persistence. Methods: Plasmid pSM31 was sequenced by Sanger sequencing using cloning and primer walking whereas pSM39 was sequenced by next generation s...


Emergence of non-wild-type populations towards biocides in Staphylococcus epide...

Costa, SS; Rosa, Mafalda; Holtreman, Frederico; Rodrigues, Ana Catarina; Viveiros, M; Pomba, Constança; Couto, I

[P269]; Aims: To characterize biocide susceptibility of Staphylococcus epidermidis colonizing veterinary staff; propose epidemiological cut-off (ECOFF) values; assess relationships between biocide and antibiotic resistance phenotypes.Methods: The susceptibility profiles of 112 S. epidermidis isolates from nasal colonization of veter-inary staff towards six biocides (including benzalkonium chloride, chlorhexidin...


Biocide susceptibility in Staphylococcus epidermidis causing infection in pets

Costa, SS; Rosa, Mafalda; Belas, Adriana; Viveiros, M; Pomba, Constança; Couto, I

[P280]; Aim: To characterize biocide susceptibility of Staphylococcus epidermidis isolates causing infection in pets and evaluate proposed biocide epidemiological cut-off (tentative ECOFF) values. Methods: The study comprised a collection of 17 S. epidermidis isolates collected from several infection sources in cats and dogs from 2001 to 2016. Susceptibility to benzalkonium chloride (BAC), cetrim-ide, chlorhexi...


Development of multidrug resistance in staphylococci driven by efflux

Costa, SS; Rosato, Adriana E.; Viveiros, M; Couto, I

[P282]; Aim: To study the efflux driven response of two major staphylococcal pathogens, S. aureus and S. epi-dermidis to the challenge by non-antibiotic drugs. Methods: We adapted three reference strains to ethidium bromide (EtBr), a broad substrate of bacte-rial efflux pumps. The parental strains, S. aureus ATCC25923, S. epidermidis ATCC12228 and S. epider-midis RP62A were cultured in varying concentrations of...


Characterization of antimicrobial resistance in Staphylococcus epidermidis colo...

Costa, SS; Rosa, Mafalda; Rodrigues, Ana Catarina; Santos, Cláudia Mineiro; Holtreman, Frederico; Viveiros, M; Pomba, Constança; Couto, I


Antibiotic and biocide resistance in Staphylococcus epidermidis colonizing huma...

Costa, SS; Rosa, Mafalda; Rodrigues, Ana Catarina; Holtreman, Frederico; Viveiros, M; Pomba, Constança; Couto, I

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