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Saccharomyces cerevisiae as a host for chondroitin production

Chondroitin is a glycosaminoglycan that has gained widespread use in nutraceuticals and pharmaceuticals, mainly for treating osteoarthritis. Traditionally, it has been extracted from animal cartilage but recently, biotechnological processes have emerged as a commercial alternative to avoid the risk of viral or prion contamination and offer a vegan-friendly source. Typically, these methods involve producing the ...

Optimization of chondroitin production in E. coli using genome scale models

Chondroitin is a natural occurring glycosaminoglycan with applications as a nutraceutical and pharmaceutical ingredient and can be extracted from animal tissues. Microbial chondroitin-like polysaccharides emerged as a safer and more sustainable alternative source. However, chondroitin titers using either natural or recombinant microorganisms are still far from meeting the increasing demand. The use of genome-sc...

Sustainable exopolysaccharide production by rhizobium viscosum CECT908 using co...

Microbial exopolysaccharides (EPS) are promising alternatives to synthetic polymers in a variety of applications. Their high production costs, however, limit their use despite their outstanding properties. The use of low-cost substrates such as agro-industrial wastes in their production, can help to boost their market competitiveness. In this work, an alternative low-cost culture medium (CSLM) was developed for...

Heterologous production of Chondroitin

Chondroitin sulfate (CS) is a glycosaminoglycan with a broad range of applications being a popular dietary supplement for osteoarthritis. Usually, CS is extracted from animal sources. However, the known risks of animal products use have been driving the search for alternative methods and sources to obtain this compound. Several pathogenic bacteria naturally produce chondroitin-like polysaccharides through well-...

Cloning, expression and characterization of UDP-glucose dehydrogenases

Uridine diphosphate-glucose dehydrogenase (UGD) is an enzyme that produces uridine diphosphate-glucuronic acid (UDP-GlcA), which is an intermediate in glycosaminoglycans (GAGs) production pathways. GAGs are generally extracted from animal tissues. Efforts to produce GAGs in a safer way have been conducted by constructing artificial biosynthetic pathways in heterologous microbial hosts. This work characterizes n...

Metabolic engineering of Escherichia coli for biotechnological chondroitin prod...

[Excerpt] Background: Chondroitin is a glycosaminoglycan that is usually extracted from animal cartilage. It has been widely used in dietary supplements for osteoarthritis due to its anti-inflammatory properties. Microbial fermentation has been evaluated as a promising, safer and more sustainable source for this compound. The most studied microorganism for biotechnological production of chondroitin is the patho...

Alternative enzymes for glycosaminoglycans biosynthetic pathway design

UDP-glucuronic acid heterologous production in Escherichia coli and Saccharomyc...

Glycosaminoglycans (GAG) such as hyaluronic acid and chondroitin sulfate are nutraceuticals with important medical and cosmetic applications. Usually, these compounds are extracted from animal sources, though concerns on animal products use are driving the search for alternative GAG sources. Some pathogenic bacterial strains naturally produce GAG like polysaccharides through identified pathways which have been ...

The biopolymer produced by Rhizobium viscosum CECT 908 is a promising agent for...

Polymer flooding is one of the most promising techniques used to increase the productivity of mature oil reservoirs. Polymers reduce the mobility ratio of the injected water relative to the crude oil, improving the displacement of the entrapped oil and consequently, increasing oil recovery. Biopolymers such as xanthan gum have emerged as environmentally friendly alternatives to the chemical polymers commonly em...

Development of a low-cost culture medium for biopolymer production by Rhizobium...

Polymers are a versatile class of compounds that play an essential role in our society, being their production estimated in more than 180 million tons per year. Nowadays, the world market is dominated by synthetic and plant-derived polymers. Biopolymers of microbial origin are characterized by their better environmental compatibility and biodegradability when compared with the synthetic ones, and their producti...