87 documents found, page 1 of 9
De novo assembly of Phlomis purpurea after challenging with Phytophthora cinnamomi
Baldé, Aladje; Neves, Dina; García-Breijo, Francisco J; Pais, Maria S; Cravador, A.Background Phlomis plants are a source of biological active substances with potential applications in the control of phytopathogens. Phlomis purpurea (Lamiaceae) is autochthonous of southern Iberian Peninsula and Morocco and was found to be resistant to Phytophthora cinnamomi. Phlomis purpurea has revealed antagonistic effect in the rhizosphere of Quercus suber and Q. ilex against P. cinnamomi. Phlomis purpurea...
Nothophytophthora gen. nov., a new sister genus of Phytophthora from natural an...
Jung, Thomas; Scanu, B.; Bakonyi, J.; Seress, D.; Kovács, G.M.; Durán, A.; Sanfuentes von Stowasser, E.; Schena, L.; Mosca, S.; Thu, P.Q.; Nguyen, C.M.During various surveys of Phytophthora diversity in Europe, Chile and Vietnam slow growing oomycete isolates were obtained from rhizosphere soil samples and small streams in natural and planted forest stands. Phylogenetic analyses of sequences from the nuclear ITS, LSU, β-tubulin and HSP90 loci and the mitochondrial cox1 and NADH1 genes revealed they belong to six new species of a new genus, officially describe...
Widespread Phytophthora infestations in European nurseries put forest, semi-nat...
Jung, Thomas; Orlikowski, L.; Henricot, B.; Abad-Campos, P.; Aday, A. G.; Aguin Casal, O.; Bakonyi, J.; Cacciola, S. O.; Cech, T.; Chavarriaga, D.An analysis of incidence of Phytophthora spp. in 732 European nurseries producing forest transplants, larger specimen trees, landscape plants and ornamentals, plus 2525 areas in which trees and shrubs were planted, is presented based on work conducted by 38 research groups in 23 European countries between 1972 and 2013. Forty-nine Phytophthora taxa were recorded in 670 nurseries (91.5%); within these nurseries,...
Induction of defence responses by cinnamomins against Phytophthora cinnamomi in...
Cravador, A.; Martins, JorgeThe strong association between Phytophthora cinnamomi and the mortality and decline of Quercus suber and Q. ilex subsp. rotundifolia has been known for two decades. The ability of elicitins secreted by this pathogen to trigger defence responses in these Quercus against itself was evaluated in this work. Biomass quantification by quantitative real-time PCR revealed a significant decrease in pathogen colonization...
Cloning, characterization and in vitro and in planta expression of a glucanase ...
Martins, I.; Martins, F.; Belo, H.; Vaz, M.; Carvalho, M.; Cravador, A.; Choupina, A.Oomycetes from the genus Phytophthora are fungus-like plant pathogens that are devastating for agriculture and natural ecosystems. They are able to secrete a glucanase inhibitor protein (GIP) that inhibits the activity of endoglucanases (EGases) involved in defense responses against infection. One of the most widely distributed and aggressive Phytophthora species, with more than 1,000 host plants is P. cinnamom...
Quantitative RT-PCR analysis of differentially expressed genes in Quercus suber...
Ebadzad, G.; Cravador, A.cDNA-AFLP methodology was used to gain insight into gene fragments differentially present in the mRNA profiles of Quercus suber roots infected with zoospores of Phytophthora cinnamomi at different post challenge time points. Fifty-three transcript-derived fragments (TDFs) were identified and sequenced. Six candidate genes were selected based on their expression patterns and homology to genes known to play a rol...
A comprehensive assessment of the transcriptome of cork oak (Quercus suber) thr...
Pereira-Leal, José B.; Abreu, Isabel A.; Alabaça, Cláudia S.; Almeida, Maria H.; Almeida, Paulo; Almeida, Tânia; Amorim, Maria I.; Araújo, SusanaBackground: Cork oak (Quercus suber) is one of the rare trees with the ability to produce cork, a material widely used to make wine bottle stoppers, flooring and insulation materials, among many other uses. The molecular mechanisms of cork formation are still poorly understood, in great part due to the difficulty in studying a species with a long life-cycle and for which there is scarce molecular/genomic inform...
Anti-Phytophthora cinnamomi activity of Phlomis purpurea plant and root extracts
Neves, D.; Caetano, P.; Oliveira, J. V.; Maia, Cristiana; Sousa, N.; Salgado, M.; Dionisio, L.; Magan, N.; Cravador, A.Phlomis purpurea (Lamiaceae), found in Quercus suber and Quercus ilex ssp. rotundifolia forest habitats in southern Portugal, is a non-host for the oomycete Phytophthora cinnamomi, the main biotic factor involved in cork oak and holm oak decline in the Iberian Peninsula. The effect of P. purpurea crude ethanol root extract was evaluated in vitro on P. cinnamomi mycelial growth, sporangial production, zoospore r...
Strategies of attack and defence in woody plant-Phytophthora interactions
Oßwald, W.; Fleischmann, F.; Rigling, D.; Coelho, A. C.; Cravador, A.; Diez, J.; Dalio, R. J.; Horta Jung, Marília; Pfanz, H.; Robin, C.; Sipos, G.This review comprises both well-known and recently described Phytophthora species and concentrates on Phytophthora–woody plant interactions. First, comprehensive data on infection strategies are presented which were the basis for three models that explain invasion and spread of Phytophthora pathogens in different woody host plants. The first model describes infection of roots, the second concentrates on invasio...
Association of polymorphism of the ß(1, 4)-galactosyltransferase-I gene with mi...
Shahbazkia, Homayon Reza; Aminlari, Mahmoud; Cravador, A.The b(1,4)-galactosyltransferase-I gene (b4galt1) encodes the catalytic part of the enzyme lactose synthase, responsible of lactose synthesis in the mammary gland. The complete coding region of the gene was screened for the presence of allelic variation among a sample of 1,200 Iranian Holstein cows, using PCR-SSCP technique followed by sequencing. Nine polymorphic nucleotide sites were identified- one in exons ...