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Amplified Sensitivity in SERS Detection of L1CAM With Silver Plasmonic Mesoporo...
Castaño-Guerrero, Yuselis; Arjones-Fernández, Belén; Moreira, Felismina T. C.; Alvarez-Puebla, Ramon A.; Correa-Duarte, Miguel A.; Águas, H.This study presents a novel approach for dual detection, leveraging a combination of a Raman reporter-bearing nanomaterial and molecular imprinting polymers (MIP). A core-shell Au-Ag nanoparticles (Au-Ag NPs) encapsulated in mesoporous silica nanocapsules (Au-Ag NCs) and a new MIP-based material targeting L1CAM are used. The MIP prepared via surface imprinting on a carbon screen-printed electrode (C-SPE) used t...
Breaking the classics: Next-generation biosensors for the isolation, profiling ...
Vaz, Raquel; Serrano, Verónica M.; Castaño-Guerrero, Yuselis; Cardoso, Ana R.; Frasco, Manuela F.; Sales, M. Goreti F.Extracellular vesicles (EVs) contain biomarkers that may represent a paradigm shift in timely disease diagnosis and personalized therapeutic approaches. Despite tremendous progress in this field, the considerable complexity and heterogeneity of EVs, combined with hurdles in isolation and accurate characterization, have delayed their envisioned clinical translation. At the same time, emerging biosensor technolog...
Poly(Thionine)-Modified Screen-Printed Electrodes for CA 19-9 Detection and Its...
Castaño-Guerrero, Yuselis; Romaguera-Barcelay, Yonny; Moreira, Felismina Teixeira Coelho; Brito, Walter Ricardo; Fortunato, ElviraPolythionine (PTH) is an electroactive compound known for its excellent electron transfer capacity. It has stable and redox centers in its structure, and it can also be generated by electropolymerization of thionine (TH). Due to its properties, it has been used in a large number of applications, including the construction of electrochemical biosensors. In this work, PTH is explored for its ability to generate e...
Poly(thionine)-modified screen-printed electrodes for CA 19-9 detection and its...
Castaño-Guerrero, Yuselis; Barcelay, Yonny Romaguera; Moreira, Felismina Teixeira Coelho; Brito, Walter Ricardo; Fortunato, ElviraPolythionine (PTH) is an electroactive compound known for its excellent electron transfer capacity. It has stable and redox centers in its structure, and it can also be generated by electropolymerization of thionine (TH). Due to its properties, it has been used in a large number of applications, including the construction of electrochemical biosensors. In this work, PTH is explored for its ability to generate e...
Breaking the classics: next-generation biosensors for the isolation, profiling ...
Vaz, Raquel; Serrano, Verónica M.; Castaño-Guerrero, Yuselis; Cardoso, Ana R.; Frasco, Manuela Faria; Sales, M. Goreti F.Extracellular vesicles (EVs) contain biomarkers that may represent a paradigm shift in timely disease diagnosis and personalized therapeutic approaches. Despite tremendous progress in this field, the considerable complexity and heterogeneity of EVs, combined with hurdles in isolation and accurate characterization, have delayed their envisioned clinical translation. At the same time, emerging biosensor technolog...
SERS and electrochemical impedance spectroscopy immunoassay for carcinoembryoni...
Castaño-Guerrero, Yuselis; Moreira, Felismina; Sousa-Castillo, Ana; Correa-Duarte, Miguel A.; Sales, GoretiThis work describes an innovative dual detection approach, combining electrochemical and surface-enhanced Raman scattering (SERS) sequential readings, on the same sensing surface. This was achieved by establishing (i) an antibody binding stage on a suitably modified screen-printed electrode (Au-SPE), to produce an electrochemical signalling system, followed by (ii) a second antibody binding stage, on the same s...
Data Employed in the Construction of a Composite Protein Database for Proteogen...
Almeida, Daniela; Domínguez-Pérez, Dany; Matos, Ana; Agüero-Chapin, Guillermin; Castaño-Guerrero, Yuselis; Vasconcelos, Vitor; Campos, AlexandreHere we provide all datasets and details applied in the construction of a composite protein database required for the proteogenomic analyses of the article “Putative Antimicrobial Peptides of the Posterior Salivary Glands from the Cephalopod Octopus vulgaris Revealed by Exploring a Composite Protein Database”. All data, subdivided into six datasets, are deposited at the Mendeley Data repository as follows. Data...
Avoiding the interference of doxorubicin with MTT measurements on the MCF-7 bre...
Luis, Carla; Castaño-Guerrero, Yuselis; Soares, Raquel; Sales, M. G. F.; Fernandes, RúbenDoxorubicin (DOXO) is an adjuvant chemotherapy agent and is also commonly used in cell biology research. Cytotoxic assays in cell culture are frequently used in order to stablish drug concentrations that are useful for controlling cell proliferation. One common cytotoxic method used is 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT). Our present research aims to support future studies in enga...
Avoiding the interference of doxorubicin with MTT measurements on the MCF-7 bre...
Luís, Carla; Castaño-Guerrero, Yuselis; Soares, Raquel; Sales, Goreti; Fernandes, RúbenDoxorubicin (DOXO) is an adjuvant chemotherapy agent and is also commonly used in cell biology research. Cytotoxic assays in cell culture are frequently used in order to stablish drug concentrations that are useful for controlling cell proliferation. One common cytotoxic method used is 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT). Our present research aims to support future studies in enga...