11 documents found, page 1 of 2
Editorial: Disease-on-a-chip: from point-of-care to personalized medicine
Cruz, Andrea; Fernandes, Elisabete; Rodrigues, Raquel Oliveira; Catarino, Susana Oliveira; Pinho, Diana[Excerpt] Introduction Miniaturization has played a pivotal role in driving innovation in microfluidic devices, particularly in their application across advanced technologies, such as Lab-on-chip platforms, Point-of-care systems, Organ-, Body- and Disease-on-a-chip technology. The personalized nature inherent in these systems, coupled with physiologically relevant readouts, opens novel avenues for individualize...
Non-invasive diagnosis and monitoring tool of children’s mental health: a point...
Cruz, Andrea; Vieira, Maria; Mesquita, Ana Raquel Marcelino; Sampaio, Adriana; Mendes-Pinto, Inês; Soares, Isabel; Freitas, Paulo P.Mental disorders are commonly featured as chronic conditions with often onset during childhood. In this context, inflammation has been associated with a higher risk of developing physical and mental health problems. Interleukin (IL)-6 is a key mediator of inflammatory responses and plays a pivotal role in immune and nervous system interaction. High levels of IL-6 during childhood are associated with mental prob...
IL-17A promotes intracellular growth of Mycobacterium by inhibiting apoptosis o...
Cruz, Andrea; Ludovico, Paula; Torrado, Egídio; Gama, José B.; Sousa, Jeremy Nicolas Carvalho; Gaifem, Joana; Appelberg, RuiThe fate of infected macrophages is a critical aspect of immunity to mycobacteria. By depriving the pathogen of its intracellular niche, apoptotic death of the infected macrophage has been shown to be an important mechanism to control bacterial growth. Here, we show that IL-17 inhibits apoptosis of Mycobacterium bovis BCG- or Mycobacterium tuberculosis-infected macrophages thus hampering their ability to contro...
Mycobacterium tuberculosis strains are differentially recognized by TLRs with a...
Carmona, J. A.; Cruz, Andrea; Teixeira, Lucia de Fatima Moreira; Sousa, Carole Lara Veiga de; Sousa, Jeremy Nicolas Carvalho; Osório, Nuno S.Tuberculosis associates with a wide spectrum of disease outcomes. The Beijing (Bj) lineage of Mycobacterium tuberculosis (Mtb) is suggested to be more virulent than other Mtb lineages and prone to elicit non-protective immune responses. However, highly heterogeneous immune responses were reported upon infection of innate immune cells with Bj strains or stimulation with their glycolipids. Using both in vitro and...
TLR2 deficiency by compromising p19 (IL-23) expression limits T helper 17 cell ...
Coelho, Maria Teixeira; Cruz, Andrea; Carmona, J. A.; Sousa, Carole Lara Veiga de; Pereira, Daniela Maria Ramos; Saraiva, Ana Laura; Veldhoen, MarcCD4+ Th1 cells producing IFN-γ are of extreme importance in controlling infections by Mycobacterium tuberculosis both in mice and in men. In addition to IFN-γ-producing T cells, IL-17-producing T cells (Th17) have been observed during mycobacterial infections. Nevertheless, their contribution for the host immune response to mycobacteria as well as the signals triggering M. tuberculosis -specific Th17 cell diffe...
Mycobacterium ulcerans Triggers T-cell immunity followed by local and regional ...
Fraga, Alexandra G.; Cruz, Andrea; Martins, Teresa G.; Torrado, Egídio; Saraiva, Margarida; Pereira, Daniela Maria Ramos; Meyers, Wayne M.Buruli ulcer (BU) is a neglected infectious disease caused by Mycobacterium ulcerans, characterized by necrotic cutaneous lesions induced by the exotoxin mycolactone. Despite evidence of Th1-mediated protective immunity, M. ulcerans infection has been associated with systemic immunosuppression. We show that early during mouse infection with either mycolactone-positive or negative strains, pathogen-specific IFN-...
Pathological role of interleukin 17 in mice subjected to repeated BCG vaccinati...
Cruz, Andrea; Fraga, Alexandra G.; Fountain, Jeffrey J.; Rangel-Moreno, Javier; Torrado, Egídio; Saraiva, Margarida; Pereira, Daniela Maria RamosInfection usually leads to the development of acquired immune responses associated with clearance or control of the infecting organism. However, if not adequately regulated, immune-mediated pathology can result. Tuberculosis is a worldwide threat, and development of an effective vaccine requires that the protective immune response to Mycobacterium tuberculosis (Mtb) be dissected from the pathological immune res...
The selective COX-2 inhibitor etoricoxib reduces acute inflammatory markers in ...
Rodrigues, Manuel Lima; Lamas, Nuno Jorge; Fernandes, Ana Valle; Cruz, Andrea; Vieira, Artur Jorge Gomes; Oliveira, Pedro; Pedrosa, JorgeOBJECTIVE: A randomised experimental study was used to evaluate the therapeutic effect of a selective cyclooxygenase-2 (COX-2) inhibitor in neurogenic laryngitis. MATERIALS AND METHODS: Male Wistar Han rats were subjected to the nasogastric intubation model (NGI) of laryngitis for 1 and 2 weeks. The NGI animals were divided into three groups: (1) treated with COX-2 inhibitor Etoricoxib, (2) vehicle and (3) non-...
A new model of laryngitis: neuropeptide, cyclooxygenase, and cytokine profile
Rodrigues, Manuel Lima; Fernandes, Ana Valle; Lamas, Nuno Jorge; Cruz, Andrea; Baltazar, Fátima; Milanezi, Fernanda; Nunes, Rui; Reis, R. M.Objectives/Hypothesis: To develop and characterize a new model of laryngeal inflammation by analyzing the presence of neurogenic peptides and expression of cyclooxygenases (COX) and cytokines in the mucosa.Study Design: Laryngitis induced by nasogastric intubation (NGI) was evaluated by histopathologic changes of the mucosa, alterations in calcitonin gene related peptide (CGRP) and substance P (SP) neuropeptide...
Understanding BCG vaccination: implications for the design of new preventive st...
Cruz, AndreaWorldwide, Mycobacterium tuberculosis infections are responsible for approximately 2 million deaths each year and the control of tuberculosis remains one of the most important challenges in the fight against infectious disease. The struggle against this mycobacterial infection has been performed using the vaccine M. bovis Bacille Calmette-Guérin (BCG) and the administration of anti-mycobacterial drugs. However ...