15 documents found, page 1 of 2
Microexon gene transcriptional profiles and evolution provide insights into blo...
Li, Xiao Hong; DeMarco, Ricardo; Neves, Leandro Xavier; James, Sally R.; Newling, Katherine; Ashton, Peter D.; Cao, Jian Ping; Wilson, R. AlanAdult schistosomes have a well-developed alimentary tract comprising an oral sucker around the mouth, a short esophagus and a blind ending gut. The esophagus is not simply a muscular tube for conducting blood from the mouth to gut but is divided into compartments, surrounded by anterior and posterior glands, where processing of ingested blood is initiated. Self-cure of rhesus macaques from a Schistosoma japonic...
Vaccination with enzimatically cleaved GPI-anchored proteins from Schistosoma m...
Martins, Vicente de Paulo; Pinheiro, Carina da Silva; Figueiredo, Bárbara de Castro Pimentel; Assis, Natan Raimundo Gonçalves deThe flatworm Schistosoma mansoni is a blood fluke parasite that causes schistosomiasis, a debilitating disease that occurs throughout the developing world. Current schistosomiasis control strategies are mainly based on chemotherapy, but many researchers believe that the best long-termstrategy to control schistosomiasis is through immunization with an antischistosomiasis vaccine combined with drug treatment. In ...
What’s in SWAP? Abundance of the principal constituents in a soluble extract of...
Neves, Leandro Xavier; Sanson, Ananda Lima; Wilson, R. Alan; Borges, William de CastroBackground: The soluble antigen preparation of adult schistosomes (SWAP) has often been used to probe host responses against these blood-dwelling parasites. Despite its long established use there is limited knowledge about its composition. The information we provide here on the molecular composition of SWAP may contribute as a guide for a rational selection of antigenic targets. Methods: Label-free quantitative...
Do schistosome vaccine trials in mice have an intrinsic flaw that generates spu...
Wilson, R. Alan; Li, Xiao Hong; Borges, William de CastroThe laboratory mouse has been widely used to test the efficacy of schistosome vaccines and a long list of candidates has emerged from this work, many of them abundant internal proteins. These antigens do not have an additive effect when co-administered, or delivered as SWAP homogenate, a quarter of which comprises multiple candidates; the observed protection has an apparent ceiling of 40–50 %. We contend that t...
Abundance of tegument surface proteins in the human blood fluke Schistosoma man...
Borges, William de Castro; Simpson, Deborah M.; Dowle, Adam A.; Curwen, Rachel S.; Oates, Jane Thomas; Beynon, Robert J.; Wilson, R. AlanThe schistosome tegument provides a major interface with the host blood stream in which it resides. Our recent proteomic studies have identified a range of proteins present in the complex tegument structure, and two models of protective immunity have implicated surface proteins as mediating antigens. We have used the QconCAT technique to evaluate the relative and absolute amounts of tegument proteins identified...
The schistosome eesophageal gland : initiator of blood processing.
Li, Xiao Hong; Borges, William de Castro; Manuel, Sophie J. Parker; Vance, Gillian M.; DeMarco, Ricardo; Neves, Leandro Xavier; Evans, Garet J. O.Background: Although the ultrastructure of the schistosome esophageal gland was described .35 years ago, its role in the processing of ingested blood has never been established. The current study was prompted by our identification of MEG-4.1 expression in the gland and the observation of erythrocyte uncoating in the posterior esophagus. Methodology/Principal Findings: The salient feature of the posterior esopha...
Characterization of phosphodiesterase-5 as a surface protein in the tegument of...
Rofatto, Henrique Krambeck; Tararam, Cibele Aparecida; Borges, William de Castro; Wilson, R. Alan; Leite, Luciana Cesar de CerqueiraSchistosoma mansoni is a major causative agent of schistosomiasis, an important parasitic disease that constitutes a severe health problem in developing countries. Even though an effective treatment exists, it does not prevent re-infection and the development of an effective vaccine still remains the most desirable means of control for this disease. In thisworkwe describe the cloning and characterization of a S...
Exploring the Fasciola hepatica tegument proteome.
Wilson, R. Alan; Wright, Janelle M.; Borges, William de Castro; Manuel, Sophie J. Parker; Dowle, Adam A.; Ashton, Peter D.; Young, Neil D.The surface tegument of the liver fluke Fasciola hepatica is a syncytial cytoplasmic layer bounded externally by a plasma membrane and covered by a glycocalyx, which constitutes the interface between the parasite and its ruminant host. The tegument’s interaction with the immune system during the fluke’s protracted migration from the gut lumen through the peritoneal cavity and liver parenchyma to the lumen of th...
Enzymatic shaving of the tegument surface of live schistosomes for proteomic an...
Borges, William de Castro; Dowle, Adam A.; Curwen, Rachel S.; Oates, Jane Thomas; Wilson, R. AlanBackground: The membrane-associated and membrane-spanning constituents of the Schistosoma mansoni tegument surface, the parasite’s principal interface with the host bloodstream, have recently been characterized using proteomic techniques. Biotinylation of live worms using membrane-impermeant probes revealed that only a small subset of the proteins was accessible to the reagents. Their position within the multil...
The proteasome-ubiquitin pathway in the Schistosoma mansoni egg has development...
Mathieson, William; Borges, William de Castro; Wilson, R. AlanSchistosoma mansoni eggs, consisting of an ovum surrounded by nutritive vitelline cells packaged in a tanned protein shell, are produced by paired worms residing in the mesenteric veins of the human host. The vitelline cells are degraded as the larval miracidium matures, the fully developed egg either crossing the gut wall to escape the host or becoming lodged in the host’s tissues where it dies and disintegrat...