7 documents found, page 1 of 1
Mucocutaneous mycoses in AIDS patients hospitalised in a tertiary healthcare ce...
Schaeffer, Lindomar; Rossicléia,; Lucilaide, Oliveira Santos,; Sousa, Diego Rayan Teixeira de; Cruz, Kátia Santana; Souza, João Vicente Braga de
Screening and antifungal activity of a β-carboline derivative against cryptococ...
Cruz, Kátia Santana; Lima, Emerson Silva; Silva, Marcia de Jesus Amazonas da; Souza, Érica Simplício de; Montoia, Andréia; Pohlit, Adrian MartinBackground. Cryptococcosis is a fungal disease of bad prognosis due to its pathogenicity and the toxicity of the drugs used for its treatment. The aim of this study was to investigate the medicinal potential of carbazole and β-carboline alkaloids and derivatives against Cryptococcus neoformans and C. gattii. Methods. MICs were established in accordance with the recommendations of the Clinical and Laboratory Sta...
MLST reveals a clonal population structure for Cryptococcus neoformans molecula...
Rocha, Diego Fernando Silva; Cruz, Kátia Santana; Silva Santos, Carla Silvana da; Stephanny Fernandes Menescal, Lizandra; Silva Neto, João Ricardo daCryptococcosis is considered endemic in Amazonas state, occurring more frequently in individuals with AIDS, who are predominantly infected by Cryptococcus neoformans molecular type VNI. Infections by Cryptococcus gattii VGII predominate in immunocompetent hosts from the American continent and are associated with outbreaks in North America, particularly the subtypes VGIIa and VGIIb, which are also present in the...
Fluorescent in situ hybridization of pre-incubated blood culture material for t...
Silva, Roberto Moreira da; Silva Neto, João Ricardo da; Santos, Carla Silvana; Cruz, Kátia Santana; Frickmann, Hagen; Poppert, Sven; Koshikene, DanielaFluorescence in situ hybridization (FISH) has been shown to be useful for the detection of Candida and Cryptococcus species in blood culture materials. FISH procedures for the detection of Histoplasma capsulatum var. capsulatum have not been reported so far. This study describes the development and evaluation of fluorescently labeled rRNA-targeting FISH probes to detect and identify H. capsulatum in blood cultu...
Antifungal and Antiplasmodial Activity of Isolated Compounds from Handroanthus ...
Melo, Loretta Ennes Sabóia de; Cruz, Kátia Santana; Lima, Soares, Pedro Igor; Nascimento, Claudete Catanhede Do; Souza, João Vicente Braga de
PCR-RFLP as a useful tool for diagnosis of invasivemycoses in a healthcare faci...
Sousa, Diego Rayan Teixeira de; Silva Santos, Carla Silvana da; Wanke, Bodo; Silva, Roberto Moreira da; dos Santos, Marcelo CordeiroBackground: The incidence of invasive mycoses is increasing worldwide. PCR-RFLP was applied to the identification of 10 reference strains and 90 cultures of agents of invasive mycoses. In addition, the new approach was applied to detect fungal agents in 120 biological samples (blood, cerebrospinal fluid and bone marrow). PCR-RFLP results were compared with the ones obtained with conventional methods (culture, m...
Evaluation of fluorescence in situ hybridisation (FISH) for the detection of fu...
Silva, Roberto Moreira da; Silva Neto, João Ricardo da; Santos, Carla Silvana; Frickmann, Hagen; Poppert, Sven; Cruz, Kátia Santana; Koshikene, DanielaThe aim of this study was to evaluate the diagnostic performance of in-house FISH (fluorescence in situ hybridisation) procedures for the direct identification of invasive fungal infections in blood cultures and cerebrospinal fluid (CSF) samples and to compare these FISH results with those obtained using traditional microbiological techniques and PCR targeting of the ITS1 region of the rRNA gene. In total, 112 ...