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Multiproduct microalgae biorefineries mediated by ionic liquids

Eppink, Michel H. M.; Ventura, Sónia P. M.; Coutinho, João A. P.; Wijffels, Rene H.

Ionic liquids (ILs) are salts with low melting points that can be used as solvents for mild extraction and selective fractionation of biomolecules (e.g., proteins, carbohydrates, lipids, and pigments), enabling the valorisation of microalgal biomass in a multiproduct biorefinery concept, while maintaining the biomolecules' structural integrity and activity. Aqueous biphasic systems and emulsions stabilised by c...


Fucoxanthin production from Tisochrysis lutea and Phaeodactylum tricornutum at ...

Pereira, Hugo; Sá, Marta; Maia, Inês Beatriz; Rodrigues, Alexandre; Teles, Iago; Wijffels, Rene H.; Navalho, João; Barbosa, Maria

Fucoxanthin is a xanthophyll carotenoid with high market value. Currently, seaweeds are the primary source for fucoxanthin industrial production. However, marine microalgae reach 5 to 10 times higher concentrations (2.24 to 26.6 mg g-1 DW) and are considered a promising feedstock. In this work, two marine microalgae were produced at industrial scale to evaluate biomass and fucoxanthin production: Phaeodactylum ...


Temperature-dependent lipid accumulation in the polar marine microalga chlamydo...

Morales-Sánchez, Daniela; Schulze, Peter S.C.; Kiron, Viswanath; Wijffels, Rene H.

The exploration of cold-adapted microalgae offers a wide range of biotechnological applications that can be used for human, animal, and environmental benefits in colder climates. Previously, when the polar marine microalga Chlamydomonas malina RCC2488 was cultivated under both nitrogen replete and depleted conditions at 8°C, it accumulated lipids and carbohydrates (up to 32 and 49%, respectively), while protein...


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