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Untargeted metabolomics reveals antidepressant effects in a marine photosynthet...

Duarte, Bernardo; Feijão, Eduardo; Cruz de Carvalho, Ricardo; Duarte, Irina A.; Marques, Ana Patrícia; Maia, Marisa; Hertzog, Jasmine; Matos, Ana Rita

The increased use of antidepressants, along with their increased occurrence in aquatic environments, is of concern for marine organisms. Although these pharmaceutical compounds have been shown to negatively affect marine diatoms, their mode of action in these non-target, single-cell phototrophic organisms is yet unknown. Using a Fourier-transform ion cyclotron-resonance mass spectrometer (FT-ICR-MS) we evaluate...


Effect Biomarkers of the Widespread Antimicrobial Triclosan in a Marine Model D...

Duarte, Bernardo; Feijão, Eduardo; Cruz de Carvalho, Ricardo; Matos, Ana Rita; Cabrita, Maria Teresa; Novais, Sara C.; Moutinho, Ariana

The present-day COVID-19 pandemic has led to the increasing daily use of antimicrobials worldwide. Triclosan is a manmade disinfectant chemical used in several consumer healthcare products, and thus frequently detected in surface waters. In the present work, we aimed to evaluate the effect of triclosan on diatom cell photophysiology, fatty acid profiles, and oxidative stress biomarkers, using the diatom Phaeoda...


Ecotoxicological effects of the anionic surfactant Sodium Dodecyl Sulfate (SDS)...

Cruz de Carvalho, Ricardo; Feijão, Eduardo; Matos, Ana; Cabrita, Maria Teresa; Utkin, Andrei; Novais, Sara; Lemos, Marco; Caçador, Isabel; Marques, João

Sodium Dodecyl Sulfate (SDS) is an anionic surfactant, extensively used in detergents, household and personal care products, as well as in industrial processes. The present study aimed to disclose the potential toxicological effects of SDS exposure under environmentally relevant concentrations (0, 0.1, 1, 3, and 10 mg L−1 ) on the physiology and biochemistry (photosynthesis, pigment, and lipid composition, anti...


Fluoxetine induces photochemistry-derived oxidative stress on Ulva lactuca

Feijão, Eduardo; Cruz de Carvalho, Ricardo; Duarte, Irina A.; Matos, Ana Rita; Cabrita, Maria Teresa; Utkin, Andrei B.; Caçador, Isabel

Emerging pollutants impose a high degree of stress on marine ecosystems, compromising valuable resources, the planet and human health. Pharmaceutical residues often reach marine ecosystems, and their input is directly related to human activities. Fluoxetine is an antidepressant, and one of the most prescribed selective serotonin reuptake inhibitors globally and has been detected in aquatic ecosystems in concent...


Effects of Glyphosate-Based Herbicide on Primary Production and Physiological F...

Cruz de Carvalho, Ricardo; Feijão, Eduardo; Matos, Ana Rita; Cabrita, Maria Teresa; Utkin, Andrei B.; Novais, Sara C.; Lemos, Marco F. L.

The use of glyphosate-based herbicides (GBHs) worldwide has increased exponentially over the last two decades increasing the environmental risk to marine and coastal habitats. The present study investigated the effects of GBHs at environmentally relevant concentrations (0, 10, 50, 100, 250, and 500 µg·L −1 ) on the physiology and biochemistry (photosynthesis, pigment, and lipid composition, antioxidative system...


Unlocking Kautsky’s dark box: development of an optical toxicity classification...

Duarte, Bernardo; Feijão, Eduardo; Cruz de Carvalho, Ricardo; Franzitta, Marco; Carlos Marques, João; Caçador, Isabel; Cabrita, Maria Teresa

Chlorophyll a induction curves, or Kautsky curves, have been extensively used to study physiological stress conditions in phototrophic organisms, with the analysis of several derived parameters. Nevertheless, these variables use only about 10 % of the information comprised in the complete Kautsky curve dataset, leaving 90 % of the photochemical data within an underutilized dark box, that is not translated into ...


Optimising Artificial Moss Growth for Environmental Studies in the Mediterranea...

Varela, Zulema; Real, Carlos; Branquinho, Cristina; do Paço, Teresa Afonso; Cruz de Carvalho, Ricardo

Bryophytes are poikilohydric organisms that play a key role in ecosystems, while some of them are also resistant to drought and environmental disturbances but present a slow growth rate. Moss culture in the laboratory can be a very useful tool for ecological restoration or the development of urban green spaces (roof and wall) in the Mediterranean region. Therefore, we aim to: (i) determine the optimal culture c...


Glyphosate-Based Herbicide Toxicophenomics in Marine Diatoms: Impacts on Primar...

Cruz de Carvalho, Ricardo; Feijão, Eduardo; Matos, Ana Rita; Cabrita, Maria Teresa; Novais, Sara C.; Lemos, Marco F. L.; Caçador, Isabel

Glyphosate is the main active component of the commercial formulation Roundup®, the most widely used chemical herbicide worldwide. However, its potential high toxicity to the environment and throughout trophic webs has come under increasing scrutiny. The present study aims to investigate the application of bio-optical techniques and their correlation to physiological and biochemical processes, including primary...


Effects of Propranolol on Growth, Lipids and Energy Metabolism and Oxidative St...

Duarte, Bernardo; Feijão, Eduardo; Cruz de Carvalho, Ricardo; Duarte, Irina A; Silva, Marisa; Matos, Ana Rita; Cabrita, Maria Teresa; Novais, Sara C

Present demographic trends suggest a rise in the contributions of human pharmaceuticals into coastal ecosystems, underpinning an increasing demand to evaluate the ecotoxicological effects and implications of drug residues in marine risk assessments. Propranolol, a non-selective β-adrenoceptor blocker, is used worldwide to treat high blood pressure conditions and other related cardiovascular conditions. Although...


Fluoxetine Arrests Growth of the Model Diatom Phaeodactylum tricornutum by Incr...

Feijão, Eduardo; Cruz de Carvalho, Ricardo; Duarte, Irina A.; Matos, Ana Rita; Cabrita, Maria Teresa; Novais, Sara C.; Lemos, Marco F. L.

Pharmaceutical residues impose a new and emerging threat to aquatic environments and its biota. One of the most commonly prescribed pharmaceuticals is the antidepressant fluoxetine, a selective serotonin re-uptake inhibitor that has been frequently detected, in concentrations up to 40 μg L-1, in aquatic ecosystems. The present study aims to investigate the ecotoxicity of fluoxetine at environmentally relevant c...


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