25 documents found, page 1 of 3
Development of a standardized histopathology scoring system for intervertebral ...
Gullbrand, SE; Ashinsky, BG; Lai, A; Gansau, J; Crowley, J; Cunha, C; Engiles, JB; Fusellier, M; Muehleman, C; Pelletier, M; Presciutti, S; Schol, JBackground: The rabbit lumbar spine is a commonly utilized model for studying intervertebral disc degeneration and for the pre-clinical evaluation of regenerative therapies. Histopathology is the foundation for which alterations to disc morphology and cellularity with degeneration, or following repair or treatment are assessed. Despite this, no standardized histology grading scale has yet been established for t...
Circulating micrornas correlate with multiple myeloma and skeletal osteolytic l...
Moura, SR; Abreu, H; Cunha, C; Ribeiro-Machado, C; Oliveira, CM; Barbosa, MA; Marques, H; Almeida, MIMultiple myeloma (MM) is the second most frequent hematological disease and can cause skeletal osteolytic lesions. This study aims to evaluate the expression of circulating microRNAs (miR-NAs) in MM patients and to correlate those levels with clinicopathological features, including bone lesions. A panel of miRNAs associated with MM onset and progression, or with bone remodeling, was analyzed in the plasma of 82...
Functional genetic variants in atg10 are associated with acute myeloid leukemia
Castro, I; Sampaio-Marques, B; Areias, AC; Sousa, H; Fernandes, Â; Sanchez-Maldonado, JM; Cunha, C; Carvalho, A; Sainz, J; Ludovico, PAcute myeloid leukemia (AML) is the most common acute leukemia, characterized by a heterogeneous genetic landscape contributing, among others, to the occurrence of metabolic repro-gramming. Autophagy, a key player on metabolism, plays an essential role in AML. Here, we examined the association of three potentially functional genetic polymorphisms in the ATG10 gene, central for the autophagosome formation. We sc...
Therapeutic strategies for IVD regeneration through hyaluronan/SDF-1-based hydr...
Cunha, C; Pereira, CL; Ferreira, JR; Ribeiro-Machado, C; Grad, S; Santos, SG; Gonçalves, RMIntervertebral disc (IVD) degeneration involves a complex cascade of events, including degradation of the native extracellular matrix, loss of water content, and decreased cell numbers. Cell recruitment strategies for the IVD have been increasingly explored, aiming to recruit either endogenous or transplanted cells. This study evaluates the IVD therapeutic potential of a chemoattractant delivery system (HAPSDF5...
Modulation of the in vivo inflammatory response by pro-versus anti-inflammatory...
Cunha, C; Teixeira, GQ; Ribeiro-Machado, C; Leite Pereira, C; Ferreira, JR; Molinos, M; Santos, SG; Barbosa, MA; Gonçalves, RMInflammation is central in intervertebral disc (IVD) degeneration/regeneration mechanisms, and its balance is crucial to maintain tissue homeostasis. This work investigates the modulation of local and systemic inflammatory response associated with IVD degeneration/herniation by administration of PRO-versus ANTI-inflammatory treatments. Chitosan/poly-¿-glutamic acid nanocomplexes, known as pro-inflammatory (PRO)...
Macrophages down-regulate gene expression of intervertebral disc degenerative m...
Silva, AJ; Ferreira, JR; Cunha, C; Corte-Real, JV; Bessa-Gonçalves, M; Barbosa, MA; Santos, SG; Gonçalves, RMLow back pain is a highly prevalent clinical problem and intervertebral disc (IVD) degeneration is now accepted as the major pathophysiological mechanism responsible for this condition. Accumulating evidence suggests that inflammation plays a crucial role in the progression of human IVD degeneration, with macrophages being pointed as the key immune cell players in this process since their infiltration in degene...
The systemic immune response to collagen-induced arthritis and the impact of bo...
Teixeira, JH; Silva, AM; Almeida, MI; Bessa-Gonçalves, M; Cunha, C; Barbosa, MA; Santos, SGRheumatoid arthritis (RA) is a systemic disease that affects the osteoarticular system, associated with bone fragility and increased risk of fractures. Herein, we aimed to characterize the systemic impact of the rat collagen-induced arthritis (CIA) model and explore its combination with femoral bone defect (FD). The impact of CIA on endogenous mesenchymal stem/stromal cells (MSC) was also investigated. CIA indu...
MÓDULO 6 - Especialidades Cirúrgicas: 3º CURSO DE FORMAÇÃO PARA INTERNOS 2015 -...
Bento, C; Rodrigues, F; Oliveira, G; Lopes, MF; Brito, MJ; Silva, P; Pires, A; Gata, L; Mação, P; Félix, M; Santos, L; Cunha, C; Piedade, C
Profiling the circulating miRnome reveals a temporal regulation of the bone inj...
Silva, AM; Almeida, MI; Teixeira, JH; Ivan, C; Oliveira, J; Vasconcelos, DM; Neves, N; Ribeiro-Machado, C; Cunha, C; Barbosa, MA; Calin, G; Santos, SGBone injury healing is an orchestrated process that starts with an inflammatory phase followed by repair and remodelling of the bone defect. The initial inflammation is characterized by local changes in immune cell populations and molecular mediators, including microRNAs (miRNAs). However, the systemic response to bone injury remains largely uncharacterized. Thus, this study aimed to profile the changes in the ...
The inflammatory response in the regression of lumbar disc herniation
Cunha, C; Silva, A; Pereira, P; Vaz, R; Gonçalves, RM; Barbosa, MALumbar disc herniation (LDH) is highly associated with inflammation in the context of low back pain. Currently, inflammation is associated with adverse symptoms related to the stimulation of nerve fibers that may lead to pain. However, inflammation has also been indicated as the main factor responsible for LDH regression. This apparent controversy places inflammation as a good prognostic indicator of spontaneou...