26 documents found, page 1 of 3
Signature cytokine-associated transcriptome analysis of effector γδ T cells ide...
Inácio, Daniel; Amado, Tiago; Pamplona, Ana; Sobral, Daniel; Cunha, Carolina; Santos, Rita F.; Oliveira, Liliana; Rouquié, Nelly; Carmo, Alexandre M.γδ T cells producing either interleukin-17A (γδ17 cells) or interferon-γ (γδIFN cells) are generated in the mouse thymus, but the molecular regulators of their peripheral functions are not fully characterized. Here we established an Il17a-GFP:Ifng-YFP double-reporter mouse strain to analyze at unprecedented depth the transcriptomes of pure γδ17 cell versus γδIFN cell populations from peripheral lymph nodes. Wit...
The Influence of Waiting Time on Customer Satisfaction: An empirical study
Cunha, Carolina; Rodrigues, Margarida; Oliveira, Cidália; Martins, NayraPara garantir melhores resultados, as organizações precisam focar no indicador mais importante que os clientes podem fornecer: a satisfação, que pode ser afetada por fatores como o tempo de espera, resultando na adoção de medidas eficientes e eficazes para a sua redução. Assim, o objetivo deste artigo é compreender a influência do tempo de espera na satisfação dos clientes, bem como os fatores que influenciam o...
Dissection of the microRNA transcriptomes of CD4+ T cell subsets in autoimmune ...
Cunha, Carolina; Romero, Paula Vargas; Inácio, Daniel; Pais, Ana Teresa; Pelicano, Catarina; Costa, Marina; Mensurado, Sofia; Gonçalves-Sousa, NatachaMicroRNAs (miRNAs) are key regulators of CD4+ T cell differentiation, but how they contribute to the course of an autoimmune disease in vivo remains poorly studied. Given the known roles in autoimmunity of pro-inflammatory T helper 1 (Th)1 and Th17 cells, and anti-inflammatory Foxp3+ regulatory cells, we established a triple reporter mouse for Ifng, Il17 and Foxp3, and subjected it to experimental autoimmune en...
Signature cytokine-associated transcriptome analysis of effector γδ T cells ide...
Inácio, Daniel; Amado, Tiago; Pamplona, Ana; Sobral, Daniel; Cunha, Carolina; Santos, Rita F.; Oliveira, Liliana; Rouquié, Nelly; Carmo, Alexandre M.γδ T cells producing either interleukin-17A (γδ cells) or interferon-γ (γδ cells) are generated in the mouse thymus, but the molecular regulators of their peripheral functions are not fully characterized. Here we established an Il17a-GFP:Ifng-YFP double-reporter mouse strain to analyze at unprecedented depth the transcriptomes of pure γδ cell versus γδ cell populations from peripheral lymph nodes. Within a very...
Dissecting the role of microRNAs in effector versus regulatory CD4+ T cell diff...
Cunha, Carolina; Romero, Paula Vargas; Inácio, Daniel; Pais, Ana Teresa; Pelicano, Catarina; Sobral, Daniel; Costa, Marina; Mensurado, SofiaIntroduction: MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate gene expression at the post-transcriptional level. They have been implicated in the regulation of the differentiation and function of CD4+ T cell subsets, key players in host defense against pathogens, but also responsible for immune-mediated diseases depending on the correct vs incorrect balance, respectively, between pro-infla...
Dissection of the IFN-γ versus IL-17-specific transcriptomes of γδ T cells: a n...
Inácio, Daniel; Amado, Tiago; Pamplona, Ana; Sobral, Daniel; Cunha, Carolina; Lesourne, Renaud; Gomes, Anita Q.; Silva-Santos, BrunoThe crucial role of murine γδ T cells in several (patho)physiological contexts stems from a complex process of ‘developmental pre-programming’ in the thymus, after which a significant fraction of γδ T cells populate peripheral sites already endowed with the capacity to secrete either IL-17 or IFN-γ. However, despite the relevance of these γδ T cell effector subsets, we still lack knowledge on the transcriptomes...
Dissecting the IFN-g versus IL-17-specific transcriptomes of effector gd T lymp...
Inácio, Daniel; Amado, Tiago; Pamplona, Ana; Sobral, Daniel; Cunha, Carolina; Lesourne, Renaud; Gomes, Anita Q.; Silva-Santos, BrunoThe crucial role of murine γδ T cells in several (patho)physiological contexts stems from a complex process of ‘developmental preprogramming’ in the thymus, after which a significant fraction of γδ T cells populate peripheral sites already endowed with the capacity to secrete either IL-17 or IFN-γ1. However, despite the relevance of these γδ T cell effector subsets, we still lack knowledge on the transcriptomes...
microRNAs are key regulators of the development and functional differentiation ...
Inácio, Daniel; Amado, Tiago; Silva, Marta; Sobral, Daniel; Cunha, Carolina; Enguita, Francisco; Pamplona, Ana; Gomes, Anita Q.; Silva-Santos, BrunoThe ability of murine γδ T cells to rapidly produce the pro-inflammatory cytokines interleukin-17 (IL-17) or interferon-γ (IFN-γ) underlies their crucial and non-redundant roles in several (patho)physiological contexts, such as tissue homeostasis, infection, autoimmunity and cancer. This capacity stems from a complex process of ‘developmental pre-programming’ in the thymus, after which a large fraction of γδ T ...
Maternal γδ T cells shape offspring pulmonary type 2 immunity in a microbiota-d...
Papotto, Pedro H.; Yilmaz, Bahtiyar; Pimenta, Gonçalo; Mensurado, Sofia; Cunha, Carolina; Fiala, Gina; Gomes Da Costa, Daniel; Gonçalves-Sousa, NatachaImmune development is profoundly influenced by vertically transferred cues. However, little is known about how maternal innate-like lymphocytes regulate offspring immunity. Here, we show that mice born from γδ T cell-deficient (TCRδ-/-) dams display an increase in first-breath-induced inflammation, with a pulmonary milieu selectively enriched in type 2 cytokines and type 2-polarized immune cells, when compared ...
Differences in Immune-Related Genes Underlie Temporal and Regional Pathological...
Fernandes, Adelaide; Caldeira, Cláudia; Cunha, Carolina; Ferreiro, Elisabete; Vaz, Ana Rita; Brites, DoraThe prevalence of Alzheimer's disease (AD), the most common cause of age-associated dementia, is estimated to increase over the next decades. Evidence suggests neuro-immune signaling deregulation and risk genes beyond the amyloid-β (Aβ) deposition in AD pathology. We examined the temporal profile of inflammatory mediators and microglia deactivation/activation in the brain cortex and hippocampus of 3xTg-AD mice ...