8 documents found, page 1 of 1
Bioprospecting bioactive peptides in Halobatrachus didactylus Body Mucus: From ...
Cunha, Marta Fernandez; Coscueta, Ezequiel R.; Brassesco, María Emilia; Almada, Frederico; Gonçalves, David; Pintado, Maria ManuelaFish body mucus plays a protective role, especially in Halobatrachus didactylus, which inhabits intertidal zones vulnerable to anthropogenic contaminants. In silico predicted bioactive peptides were identified in its body mucus, namely, EDNSELGQETPTLR (HdKTLR), DPPNPKNL (HdKNL), PAPPPPPP (HdPPP), VYPFPGPLPN (HdVLPN), and PFPGPLPN (HdLPN). These peptides were studied in vitro for bioactivities and aggregation be...
Bioprospecting bioactive peptides in halobatrachus didactylus body mucus: from ...
Cunha, Marta Fernandez; Coscueta, Ezequiel R.; Brassesco, María Emilia; Almada, Frederico; Gonçalves, David; Pintado, Maria ManuelaFish body mucus plays a protective role, especially in Halobatrachus didactylus, which inhabits intertidal zones vulnerable to anthropogenic contaminants. In silico predicted bioactive peptides were identified in its body mucus, namely, EDNSELGQETPTLR (HdKTLR), DPPNPKNL (HdKNL), PAPPPPPP (HdPPP), VYPFPGPLPN (HdVLPN), and PFPGPLPN (HdLPN). These peptides were studied in vitro for bioactivities and aggregation be...
NanoLC-MS/MS sequencing and in silico analysis of bioactive peptides from Lusit...
Cunha, Marta Fernandez; Coscueta, Ezequiel R.; Brassesco, María Emilia; Almada, Frederico; Gonçalves, David; Pintado, ManuelaThe mucus covering a fish's body works as a protective barrier and contains molecules, such as antimicrobial peptides, that defend against pathogens. In this study, two adult Halobatrachus didactylus were captured from the wild in Sesimbra, and mucus was collected by scraping the dorsal-lateral body with a sponge. Our objective was to identify new bioactive peptides in mucus samples via chromatography. SE-HPLC ...
Sustainable bioprospecting and circular economy approach: fractionation and ide...
Cunha, Marta Fernandez; Coscueta, Ezequiel R.; Brassesco, María Emilia; Almada, Frederico; Gonçalves, David; Pintado, ManuelaBioactive molecules from marine organisms, especially the Halobatrachus didactylus, also known as the Lusitanian toadfish, native to the Portuguese coast, remains largely unexplored. It is important to use sustainable bioprospecting practices to protect both the species and its ecosystem (1). Therefore, by adopting a circular economy approach, we can maximize the sustainable use of marine resources by sampling ...
NanoLC-MS/MS sequencing and in silico analysis of bioactive peptides from Lusit...
Cunha, Marta Fernandez; Coscueta, Ezequiel R.; Brassesco, María Emilia; Almada, Frederico; Gonçalves, David; Pintado, ManuelaThe mucus covers the fish's body, working as a protective barrier. Besides physical protection, mucus provides molecules that protect the fish from damaging pathogens [1,2]. Has been reported that antimicrobial peptides are secreted in the mucus, which play an essential role in defense against microbial pathogens since these belong to the innate immune system [2,3].
Methods for the collection of fish mucus: a systematic review
Cunha, Marta Fernandez; Coscueta, Ezequiel R.; Brassesco, María Emilia; Almada, Frederico; Gonçalves, David; Pintado, ManuelaThe aquatic environment holds a vast source of organisms that provide numerous opportunities to bioprospect new molecules. Notably, fish are producers of an epidermal mucus that offers protection against pathogens, making it a promising source of bioactive molecules. This source of molecules, however, has yet to be thoroughly explored, and particularly, optimization of methods for collection and study is needed...
Exploring bioactivities and peptide content of body mucus from the lusitanian t...
Cunha, Marta Fernandez; Coscueta, Ezequiel; Emilia Brassesco, M.; Marques, Rita; Neto, José; Almada, Frederico José Oliveira de; Goncalves, DavidIdentifying bioactive molecules from marine organisms is still vastly understudied. Fish remain an untapped source of bioactive molecules, even when considering species whose toxicity to other fish species has been noticed before. We assessed potential applications of crude body mucus of the Lusitanian toadfish (Halobratachus didactylus) and characterized its peptide fraction composition. Mucus samples from thr...
Exploring bioactivities and peptide content of body mucus from the lusitanian t...
Cunha, Marta Fernandez; Coscueta, Ezequiel R.; Brassesco, María Emilia; Marques, Rita; Neto, José; Almada, Frederico; Gonçalves, David; Pintado, ManuelaIdentifying bioactive molecules from marine organisms is still vastly understudied. Fish remain an untapped source of bioactive molecules, even when considering species whose toxicity to other fish species has been noticed before. We assessed potential applications of crude body mucus of the Lusitanian toadfish (Halobratachus didactylus) and characterized its peptide fraction composition. Mucus samples from thr...