15 documents found, page 1 of 2
New insights into the structure and mode of action of Mo-CBP3, an antifungal Ch...
Batista, Adelina B.; Oliveira, José T. A.; Gifoni, Juliana M.; Pereira, Mirella L.; Almeida, Marina G. G.; Gomes, Valdirene M.; Cunha, Maura daMo-CBP3 is a chitin-binding protein purified from Moringa oleifera Lam. seeds that displays inhibitory activity against phytopathogenic fungi. This study investigated the structural properties and the antifungal mode of action of this protein. To this end, circular dichroism spectroscopy, antifungal assays, measurements of the production of reactive oxygen species and microscopic analyses were utilized. Mo-CBP3...
Purification, biochemical characterization and antifungal activity of a new lip...
Zottich, Umberto; Cunha, Maura da; Carvalho, André O.; Dias, Germana Bueno; Silva, Nádia C.M.; Santos, Izabela S.; Nacimento, Viviane V. doBackground: A growing number of cysteine-rich antimicrobial peptides (AMPs) have been isolated from plants and particularly from seeds. It has become increasingly clear that these peptides, which include lipid transfer proteins (LTPs), play an important role in the protection of plants against microbial infection. Methods: Peptides from Coffea canephora seeds were extracted in Tris–HCl buffer (pH 8.0), and chro...
Micromorfologia da superfície aérea de Psychotria nuda (Cham. & Schlecht.) Wawr...
Klein, Denise Espellet; Moraes, T. M. S.; Miguel, Emilio de Castro; Silva, A. de S. da; Alexandrino, Cristiane Duarte; Moraes, Daniel Gomes deHá cerca de 400 milhões de anos, a parede periclinal externa das primeiras plantas terrestre já era coberta por cutícula [1]. Em primeira análise, a superfície aérea das plantas, por ser uma região de fronteira com o ambiente, funciona como uma proteção contra adversidades bióticas e abióticas. A superfície dos órgãos aéreos de P. nuda, apresentase glabra, ou seja, sem tricomas, com exceção da base da corola e ...
Ultrastructure of secretory and senescence phase in colleters of Bathysa gymnoc...
Miguel, Emilio de Castro; Klein, Denise Espellet; Oliveira, Marco Antonio de; Cunha, Maura daColleters are secretory structures formed by a parenchymatic axis with vascular bundles, bound by a layer of secretory palisade-like epidermis. Some studies regarding the structure of colleters have focused on secretory cells structure, but not distinguished the secretory and senescent phases. Generally, in mucilage-secreting cells such as colleters, the endoplasmic reticulum and Golgi apparatus are involved in...
Stipular colleters in Psychotria nuda (Cham. & Schltdl.) Wawra (Rubiaceae): mic...
Miguel, Emilio de Castro; Moraes, Daniel Gomes de; Cunha, Maura daColleters are secretory structures, with a protective function, that occur in different organs and in many Angiospermae families. The aim of this work was to describe the anatomy and micromorphology of colleters present in Psychotria nuda (Cham. & Schltdl.) Wawra as well as analyze the structure and composition of crystals from the central axis of the structure under study. The material was collected at the Tin...
Ultrastructural, physiological and biochemical analyses of chlorate toxicity on...
Borges, Regis; Miguel, Emilio de Castro; Dias, Janice Maria Ribeiro; Cunha, Maura da; Bressan-Smith, Ricardo Enrique; Oliveira, Jurandi Gonçalves deChlorates are oxidizing substances considered phytotoxic to all green plant parts. In this study the physiological, biochemical and ultrastructural effects of chlorate treatments were studied in rice seedlings grown at two different temperatures (28 and 6 ◦C). Visual analyses demonstrated that the chlorate treatment provoked severe damage to plant leaves only at 28 ◦C. The electron microscopy of the chloroplast...
Vitamin K supplementation modulates bone metabolism and ultra-structure of ovar...
Rangel, Letícia Batista Azevedo; Siqueira, Daniel de; Soares, Olívia do Rosário; Santana, Higor Scardini; Miguel, Emilio de Castro; Cunha, Maura daOsteoporosis is a bone metabolic disease that affects mostly postmenopausal women. There has been shown that vitamin K (VK) supplementation during menopause may decrease bone loss as well as risk of bone breaking. Aiming to clarify the beneficial role of VK in bone metabolism during menopause, we investigated mineral metabolism and bone ultrastructure of ovariectomized (OVX) mice. Methods: To determine the effe...
Outer cell wall structure and the secretion mechanism of colleters of Bathysa n...
Miguel, Emilio de Castro; Pireda, Saulo; Barros, Claudia Franca; Zottich, Umberto; Gomes, Valdirene Moreira; Miguens, Flavio Costa; Cunha, Maura daSecretory structures are formed by many types of plants and are present on numerous diff erent organs. Among the many types of known secretory structures, colleters are predominant on plants of the Rubiaceae. One remarkable characteristic of secretory tissues is the export of exudates; however, the precise mechanism involved in this process is still unclear. To better understand the mechanisms of exudate extern...
Seed structure in Canavalia brasiliensis Mart. ex Benth. (Leguminosae) and subc...
Miguel, Emilio de Castro; Miguel, Thaiz Batista Azevedo Rangel; Pireda, S.; Marques, Jonas de Brito Campolina; Cunha, Maura da; Cajazeiras, João BatistaLectins are proteins capable of specific and reversible recognition of carbohydrates without modifying them. Many studies have isolated these molecules from Leguminosae seeds but little attention was given to subcellular localization and biological function of these molecules. Therefore, this work aimed to describe Canavalia brasiliensis seed structure and the subcellular localization of ConBr. In addition, we ...
Morphology, ultrastructure and immunocytochemistry of Hypnea cervicornis and Hy...
Miguel, Thaiz Batista Azevedo Rangel; Schmidt, Eder C.; Bouzon, Zenilda Laurita; Nascimento, Fernando E.P.; Cunha, Maura da; Fernandes, Saulo PiredaBased on their morphological and physiological features, red algae comprise a complex and variable group of multiple genera, including Hypnea. In particular, the genus Hypnea J.V. Lamouroux (Cystocloniaceae, Rhodophyta) consists of approximately 54 species, including Hypnea cervicornis and H. musciformis. Lectins were described for both species; however, the localization of these proteins is still unclear. Ther...