14 documents found, page 1 of 2
Immunogenicity of a Recombinant Gonadotropin-releasing Hormone Associated to th...
Eslabão,Lívia Budziarek; Conrad,Neida Lucia; Albuquerque,Pedro Machado Medeiros de; Cunha,Rodrigo Casquero; Varela Junior,Antônio SérgioAbstract Gonadotropin-releasing hormone (GnRH) is one of the main targets for the development of immunocontraceptives vaccines. The aim of this study was to clone and express the recombinant GnRH fused to the B subunit of Escherichia coli heat-labile enterotoxin (LTB) molecule in Pichia pastoris and Escherichia coli platforms and evaluate their immunogenicity in mice. P. pastoris (pGnRH/LTB) and E. coli (eGnRH/...
Staphylococcal slime layers and biofilm from different origins
Cunha,Rodrigo Casquero; Rosa,Michelle Dias Hornes da; Silva,Cleomar da; Santos,Francisco Denis Souza; Leite,Fábio Pereira LeivasABSTRACT: The genus Staphylococcus comprises some of the most important pathogenic bacteria for both humans and animals. It is responsible for bovine mastitis and canine otitis, besides being present in the microbiota of animals and as a contaminant in food. Its pathogenesis is related to the formation of capsule and biofilm, which contribute to its infectivity. The objective of this study was to observe the pr...
Wildlife species, Ixodid fauna and new host records for ticks in an Amazon fore...
Zimmermann,Namor Pinheiro; Aguirre,André de Abreu Rangel; Rodrigues,Vinicius da Silva; Garcia,Marcos Valério; Medeiros,Jansen FernandesAbstract The objective of this work was to evaluate the diversity of ticks associated with free-living animals and to investigate new host records for ticks. Ticks were collected from animals rescued during the flood of the Jamari River in the municipality of Ariquemes, state of Rondônia, North Region of Brazil. A total of 39 animals were captured, out of which 10 were amphibians, 19 were reptiles and 10 were m...
Analysis of Bm86 conserved epitopes: is a global vaccine against Cattle Tick Rh...
Blecha,Isabella Maiumi Zaidan; Csordas,Bárbara Guimarães; Aguirre,André de Abreu Rangel; Cunha,Rodrigo Casquero; Garcia,Marcos Valério; Andreotti,RenatoAbstract The cattle tick Rhipicephalus microplus causes significant economic losses in agribusiness. Control of this tick is achieved mainly through the application of chemical acaricides, often resulting in contamination of animal food products and of the environment. Another major concern associated with acaricide use is the increasing reports of resistance of this tick vector against the active ingredients o...
New insights from molecular characterization of the tick Rhipicephalus (Boophil...
Csordas,Bárbara Guimarães; Garcia,Marcos Valério; Cunha,Rodrigo Casquero; Giachetto,Poliana Fernanda; Blecha,Isabella Maiumi Zaidan; Andreotti,RenatoAbstract The Rhipicephalus (Boophilus) microplus complex currently consists of five taxa, namely R. australis, R. annulatus, R. (B.) microplus clade A sensu, R. microplus clade B sensu, and R. (B.) microplus clade C sensu. Mitochondrial DNA-based methods help taxonomists when they are facing the morpho-taxonomic problem of distinguishing members of the R. (B.) microplus complex. The purpose of this study was to...
Neospora caninum and Toxoplasma gondii serodiagnosis in human immunodeficiency ...
Oshiro,Leandra Marla; Motta-Castro,Ana Rita Coimbra; Freitas,Solange Zacalusni; Cunha,Rodrigo Casquero; Dittrich,Rosangela LocatelliABSTRACTINTRODUCTION:Neospora caninum and Toxoplasma gondii belong to the Sarcocystidae family, and both have one definitive and various intermediary hosts. Owing to their weak immune systems, immunocompromised persons might be prone to opportunistic infections. The aim of this study was to investigate the presence of anti- N. caninum and anti- T. gondii antibodies in immunocompromised individuals.METHODS:This ...
Recombinant gp19 as a potential antigen for detecting anti-Ehrlichia canis anti...
Oliveira,Rômulo Silva de; Cunha,Rodrigo Casquero; Moraes-Filho,Jonas; Gonçales,Relber Aguiar; Lara,Ana Paula de Souza Stori deThe canine monocytic ehrlichiosis, caused by Ehrlichia canis, is endemic in several regions of Brazil. Some serological diagnostic techniques using immunodominant proteins of E. canis as antigens are available, but their specificities and sensitivities are questionable. Based on this, the objective of this study was to test the antigenic potential of the recombinant gp19 protein (rGP19) for subsequent use in di...
Geographical distribution of Trypanosoma cruzi in triatomine vectors in the Sta...
Cominetti,Marlon Cezar; Csordas,Bárbara Guimarães; Cunha,Rodrigo Casquero; Andreotti,RenatoIntroduction This work presents the initial findings of a molecular epidemiological investigation of Trypanosoma cruzi in triatomine insects in State of Mato Grosso do Sul. Methods A total of 511 triatomines from different regions of the state were examined. Deoxyribonucleic acid (DNA) was extracted from the intestinal contents of the insects using phenol-chloroform-isoamyl alcohol (25:24:1). Polymerase chain r...
Detection of Leishmania infantum in Lutzomyia longipalpis captured in Campo Gra...
Cunha,Rodrigo Casquero; Andreotti,Renato; Cominetti,Marlon Cezar; Silva,Elaine AraújoLeishmaniasis is a zoonotic disease caused by protozoa of the genus Leishmania (Ross, 1903) and is the focus of considerable attention in human and veterinary medicine. In the city of Campo Grande, MS, the causative agent of visceral leishmaniasis is Leishmania infantum (= L. chagasi) primary vector, comprising approximately 92.9% of the local sandfly population, is Lutzomyia longipalpis. The aim of this work w...
Environmentally associated ticks (Acari: Ixodidae) in Campo Grande, Mato Grosso...
Garcia,Marcos Valério; Silva,Dayana Campelo da; Almeida,Robson Ferreira Cavalcante de; Cunha,Rodrigo Casquero; Matias,JaquelineHerein, we report tick species found on wild and domestic animals and in the environment during a one-year sampling period at the Brazilian Farming Research Company beef cattle unit (Embrapa Beef Cattle), which is located within the urban area of Campo Grande, Mato Grosso do Sul, Brazil. From 55 wild hosts including six different species (Nasua nasua, Cebus spp., Cerdocyon thous,Myrmecophaga tridactyla, Tamandu...