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Quick decline and stem pitting Citrus tristeza virus isolates induce a distinct...
Dandlen, Susana; Da Silva, José Paulo; Miguel, Maria Graca; Duarte, Amilcar; Power, Deborah; Marques, NatáliaSusceptibility to the severe Citrus tristeza virus (CTV), T36, is higher for Citrus macrophylla (CM) than for C. aurantium (CA). How host-virus interactions are reflected in host physiology is largely unknown. In this study, the profile of metabolites and the antioxidant activity in the phloem sap of healthy and infected CA and CM plants were evaluated. The phloem sap of quick decline (T36) and stem pitting (T3...
Bile acids as putative social signals in Mozambique tilapia (Oreochromis mossam...
Ashouri, Samyar; Da Silva, José Paulo; Canario, Adelino; Hubbard, PeterChemical cues provide potential mates with information about reproductive status and resource-holding potential. In the Mozambique tilapia (Oreochromis mossambicus), males can distinguish female reproductive status through chemical cues, and accessibility of males to females depends on their position in the hierarchy, determined in part by chemical cues. Here, we hypothesized that tilapia faecal cues are attrac...
LC-HRMS profiling of paralytic shellfish toxins in Mytilus galloprovincialis af...
Lage, Sandra; Reis Costa, Pedro; Canario, Adelino; Da Silva, José PauloSaxitoxin and its more than 50 analogues are a group of naturally occurring neurotoxins collectively designated as paralytic shellfish toxins (PSTs). PSTs are toxic to humans and maximum legal limits in seafood have been implemented by regulatory authorities worldwide. In the European Union, monitoring of PSTs is performed using the AOAC Official Method 2005.06, based on liquid chromatography coupled with fluor...
Effects of foliar application of organic acids on strawberry plants
Saavedra, Teresa; Gama, Florinda; Rodrigues, Maria Joao; Abadía, Javier; de Varennes, Amarilis; Pestana, Maribela; Da Silva, José PauloThe large economic costs and environmental impacts of iron-chelate treatments has led to the search for alter-native methods and compounds to control iron (Fe) deficiency chlorosis. Strawberry plants (Fragaria x ananassa) were grown in Hoagland's nutrient solution in a greenhouse with two levels of Fe: 0 and 10 mu M Fe(III)-EDDHA. After 20 days, plants growing without Fe showed typical symptoms of Fe deficiency...
Tissue accumulation of tetrodotoxin (TTX) and analogues in trumpet shell Charon...
Lage, Sandra; Afonso, Inês I.; Reis Costa, Pedro; Canario, A.V.M.; Da Silva, José PauloTetrodotoxin (TTX) is a potent neurotoxin responsible for a human intoxication event in Spain associated with the consumption of trumpet shell Charonia lampas. In Europe, TTX is not regulated or monitored, and there is little knowledge about its presence in seafood. Here, we investigated the tissue distribution of TTX and analogues in three specimens of trumpet shell C. lampas bought in a market in southern Por...
2-Hydroxychalcone−β-Cyclodextrin Conjugate with pH-Modulated Photoresponsive Bi...
Paulino, Micael; Pérez-Juste, Ignacio; Cid, María Magdalena; Da Silva, José Paulo; Pereira, M. Manuela A.; Basílio, NunoStimuli-responsive supramolecular receptors are important building blocks for the construction of self-assembled functional materials. We report the design and synthesis of a pH and light-responsive 2-hydroxychalcone-beta-cyclodextrin conjugate (1-Ct) and its characterization by spectroscopic and computational methods. 1-Ct follows the typical reaction network of trans-chalcone-flavylium photoswitches. Upon lig...
Insights into the antimicrobial activities and metabolomes of Aquimarina (Flavo...
Silva, Sandra Godinho; Paula, Patrícia; Da Silva, José Paulo; Mil-Homens, Dalila; Teixeira, Miguel Cacho; Fialho, Arsénio Mendes; Costa, RodrigoTwo novel natural products, the polyketide cuniculene and the peptide antibiotic aquimarin, were recently discovered from the marine bacterial genus Aquimarina. However, the diversity of the secondary metabolite biosynthetic gene clusters (SM-BGCs) in Aquimarina genomes indicates a far greater biosynthetic potential. In this study, nine representative Aquimarina strains were tested for antimicrobial activity ag...
Fluorescence sensing of microplastics on surfaces
Costa, Camila Q. V.; Cruz, Joana; Martins, Jorge; Teodosio, Maria; Jockusch, Steffen; Ramamurthy, V.; Da Silva, José PauloMicroplastics, nanoplastics and related products have been recently found in marine ecosystems worldwide, calling for new analytical methods for rapid detection and risk assessment. Fluorescence is a sensitive technique that when used with polarity probes can potentially detect low-polarity plastic particles in environments. Here, we evaluated the fuorescence technique to sense polystyrene microparticles direct...
A novel plant extract as a biostimulant to recover strawberry plants from iron ...
Saavedra, Teresa; Gama, Florinda; Correia, P.J.; Da Silva, José Paulo; Miguel, Maria da Graça; de Varennes, Amarilis; Pestana, MaribelaThe traditional agricultural production systems are evolving more towardorganic, sustainable or environmental friendly systems and products; how-ever, it is important to keep the yield and the quality of crops. The aim ofthis work was to evaluate the recovery of iron deficient strawberry plantsby foliar spray, using a gramineous plants extract (GE). Bare-root trans-plants of strawberry (Fragaria ananassaDuch. c...
Competing pathways for photoremovable protecting groups: the effects of solvent...
Field, Thomas; Peterson, Julie; Ma, Chicheng; Jagadesan, Pradeepkumar; Da Silva, José Paulo; Rubina, Marina; Ramamurthy, V.; Givens, Richard S.Extending the applications of Photoremovable Protecting Groups (PPGs) to "cage" phenols has generally met with unusually complex PPG byproducts. In this study, we demonstrate that thep-hydroxyphenacyl (pHP) cage for both simple and complex phenolics, including tyrosine, dispenses free phenols. With the simpler unsubstituted phenols, the reaction is governed by their Bronsted Leaving Group ability. On the other ...