8 documents found, page 1 of 1
Studying and analyzing humane endpoints in the fructose-fed and streptozotocin-...
Silva-Reis, R; Faustino-Rocha, Ana I; Silva, J; Valada, A; Azevedo, T; Anjos, L; Gonçalves, L; Pinto, ML; Ferreira, R; Silva, AM; Cardoso, SMThis study assessed a humane endpoint scoring system to detect animal suffering in a rat model of type 2 diabetes. Sprague-Dawley male rats were divided into control and induced (fructose-fed and streptozotocin (STZ) administration) groups. Induced animals drank 10% fructose for 14 days, then received STZ (40 mg/kg) intraperitoneally. Weekly monitoring of body weight, water, and food consumption, 14 parameters ...
Animal welfare in diabetes research: a humane endpoint scoring system
Faustino-Rocha, Ana I; Silva-Reis, R; Silva, J; Valada, A; Azevedo, T; Anjos, L; Silva, MAS; Cardoso, SM; Oliveira, PAHumane endpoints are increasingly being used in animal experiments to ensure compliance with ethical regulations, namely the 3Rs policy. This study aimed to develop a clear humane endpoint (HE) scoring system for a streptozotocin (STZ)-induced diabetes model in male Sprague-Dawley rats. The rats were divided into a control (n=8) and an induced (n=16) group, with the latter receiving 10% fructose in their drinki...
Animal welfare in diabetes research: a humane endpoint scoring system
Faustino-Rocha, Ana I; Silva-Reis, R; Silva, J; Valada, A; Azevedo, T; Anjos, L; Silva, MAS; Cardoso, SM; Oliveira, PAaustino-Rocha AI, Silva-Reis R, Silva J, Valada A, Azevedo T, Anjos L, Silva MAS, Cardoso SM, Oliveira PA. 2023. Animal welfare in diabetes research: a humane endpoint scoring system. 3rd International Conference on Diabetes, Endocrinology & Obesity, 20 a 21 de março.
Phenolic Bioactives as Antiplatelet Aggregation Factors: The Pivotal Ingredient...
Sharifi-Rad, J; Quispe, C; Zam, W; Kumar, M; Cardoso, SM; Pereira, OR; Ademiluyi, AO; Adeleke, O; Moreira, AC; Živkovic, J; Noriega, F; Ayatollahi, SACardiovascular diseases (CVD) are one of the main causes of mortality in the world. The development of these diseases has a specific factor-alteration in blood platelet activation. It has been shown that phenolic compounds have antiplatelet aggregation abilities and a positive impact in the management of CVD, exerting prominent antioxidant, anti-inflammatory, antitumor, cardioprotective, antihyperglycemic, and ...
Chitosan nanoparticles as a promising tool in nanomedicine with particular emph...
Sharifi-Rad, J; Quispe, C; Butnariu, M; Rotariu, LS; Sytar, O; Sestito, S; Rapposelli, S; Akram, M; Iqbal, M; Krishna, A; Kumar, NVA; Braga, SSThe study describes the current state of knowledge on nanotechnology and its utilization in medicine. The focus in this manuscript was on the properties, usage safety, and potentially valuable applications of chitosan-based nanomaterials. Chitosan nanoparticles have high importance in nanomedicine, biomedical engineering, discovery and development of new drugs. The manuscript reviewed the new studies regarding ...
Mitochondrial-dependent apoptosis in Huntington's disease human cybrids
Ferreira, IL; Nascimento, MV; Ribeiro, MH; Almeida, S; Cardoso, SM; Grazina, M; Pratas, J; Santos, MJ; Januário, C; Oliveira, CR; Rego, ACWe investigated the involvement of mitochondrial-dependent apoptosis in Huntington's disease (HD) vs. control (CTR) cybrids, obtained from the fusion of human platelets with mitochondrial DNA-depleted NT2 cells, and further exposed to 3-nitropropionic acid (3-NP) or staurosporine (STS). Untreated HD cybrids did not exhibit significant modifications in the activity of mitochondrial respiratory chain complexes I-...
Mitochondrial function in Parkinson's disease cybrids containing an nt2 neuron-...
Esteves, AR; Domingues, AF; Ferreira, IL; Januário, C; Swerdlow, RH; Oliveira, CR; Cardoso, SMMitochondria likely play a role in Parkinson's disease (PD) neurodegeneration. We modelled PD by creating cytoplasmic hybrid (cybrid) cell lines in which endogenous mitochondrial DNA (mtDNA) from PD or control subject platelets was expressed within human teratocarcinoma (NT2) cells previously depleted of endogenous mtDNA. Complex I activity was reduced in both PD cybrid lines and in the platelet mitochondria us...
Mitochondria dysfunction of Alzheimer's disease cybrids enhances Abeta toxicity
Cardoso, SM; Santana, I; Swerdlow, RH; Oliveira, CRAlzheimer's disease (AD) brain reveals high rates of oxygen consumption and oxidative stress, altered antioxidant defences, increased oxidized polyunsaturated fatty acids, and elevated transition metal ions. Mitochondrial dysfunction in AD is perhaps relevant to these observations, as such may contribute to neurodegenerative cell death through the formation of reactive oxygen species (ROS) and the release of mo...