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Isolation and characterization of plastid terminal oxidase gene from carrot and...

Campos, MD; Campos, C; Cardoso, Hélia; Simon, PW; Oliveira, M; Nogales, A; Arnholdt-Schmitt, B

Carrot (Daucus carota L.) is a biennial plant that accumulates considerable amounts of carotenoid pigments in the storage root. To better understand the molecular mechanisms for carotenoid accumulation in developing storage roots, plastid terminal oxidase (PTOX) cDNA was isolated and selected for reverse-transcription quantitative polymerase chain reaction (RT-qPCR). Present in photosynthetic species, PTOX is a...


Wild carrot differentiation in Europe and positive selection at DcAOX1 gene

Nobre, Tânia; Oliveira, M; Arnholdt-Schmitt, B

By definition, the domestication process leads to an overall reduction of crop genetic diversity. This lead to the current search of genomic regions in wild crop relatives (CWR), an important task for modern carrot breeding. Nowadays massive sequencing possibilities can allow for discovery of novel genetic resources in wild populations, but this quest could be aided by the use of a surrogate gene (to first iden...


Involvement of alternative oxidase (AOX) in adventitious rooting of Olea europa...

Santos Macedo, E.; Sircar, D.; Cardoso, Hélia; Peixe, A; Arnholdt-Schmitt, B

Alternative oxidase (AOX) has been proposed as a functional marker candidate in a number of events involving cell differentiation, including rooting efficiency in semi-hardwood shoot cuttings of olive (Olea europaea L.). To ascertain the general importance of AOX in olive rooting, the auxin-induced rooting process was studied in an in vitro system for microshoot propagation. Inhibition of AOX by salicylhydroxami...


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