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Effect of carbon source on somatic embryogenesis of Bactris gasipaes
Bettencourt, Gisela Manuela de França; Zanella, Laudiane Bruna; Quoirin, Marguerite Germaine Ghislaine; Degenhardt-Goldbach, JulianaThin cell layer of meristematic region was successfully used to induce somatic embryogenesis in greenhouse-grown plants of pejibaye. The effects of three sources of carbohydrates, sucrose, glucose and mannitol on induction of somatic embryogenic calli were evaluated. Somatic embryos were observed on media containing either sucrose or glucose.; A metodologia de camada fina de células foi utilizada com sucesso pa...
In vitro seed germination and multiplication of Calophyllum brasiliense
Silveira, Sheila Susy; Degenhardt-Goldbach, Juliana; Quoirin, Marguerite Germaine GhislaineCalophyllum brasiliense is a tree species with limited natural reproduction. In vitro germination may be an alternative for obtaining high-quality seedlings. Seeds were maintained in water before surface disinfestation and compared with control seeds (i.e. not immersed), without differences between treatments. HgCl2 used during surface-disinfestation reduced contamination rates of cultures. Fungal contamination...
Callus induction in vitro on leaves explants of Ilex paraguariensis
Stachevski, Thamires Weigert; Franciscon, Luziane; Degenhardt-Goldbach, JulianaOrganogenesis is a technique rarely studied in the micro propagation of Ilex paraguariensis. This study evaluated different culture media on callus induction and in vitro organogenesis of this species. Leaves were collected from greenhouse grown plants. Leaves segments were placed on culture media ¼ MS, WPM or JADS, containing zeatin and 2,4-D. The MS medium was more efficient in callus inducing. Rooting was ob...