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Potassium bromate as positive assay control for the Fpg-modified comet assay
Møller, P; Muruzabal, D; Bakuradze, T; Richling, E; Bankoglu, EE; Stopper, H; Langie, SAS; Azqueta, A; Jensen, A; Scavone, F; Giovannelli, LThe comet assay is a popular assay in biomonitoring studies. DNA strand breaks (or unspecific DNA lesions) are measured using the standard comet assay. Oxidative stress-generated DNA lesions can be measured by employing DNA repair enzymes to recognise oxidatively damaged DNA. Unfortunately, there has been a tendency to fail to report results from assay controls (or maybe even not to employ assay controls). We b...
Date: 2020
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Origin: Repositório Aberto da Universidade do Porto