15 documents found, page 1 of 2

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A Real Time PCR strategy for the detection and quantification of Candida albica...

Busser, Felipe Delatorre; Coelho, Vivian Caso; Fonseca, Claudia de Abreu; Del Negro, Gilda Maria Barbaro; Shikanai-Yasuda, Maria Aparecida

Candidemia is a significant cause of bloodstream infections (BSI) in nosocomial settings. The identification of species can potentially improve the quality of care and decrease human mortality. Quantitative PCR (qPCR) was evaluated for Candida albicans detection using culture suspensions containing C. albicans, spiked human blood, the cloned qPCR target fragment (ITS2 region) and the results of these assays wer...

Date: 2020   |   Origin: Oasisbr


Paracoccidioides brasiliensis: a mycologic and immunochemical study of a sample...

Vidal, Monica Scarpelli Martinelli; Melo, Natalina Takahashi de; Garcia, Nilma Maciel; Del Negro, Gilda Maria Barbaro; Assis, Cezar Mendes de

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T19:04:25Z No. of bitstreams: 1 S0036-46651995000100007.pdf: 897597 bytes, checksum: 5cd06ce16898f7704f543141f811f999 (MD5); Made available in DSpace on 2013-08-22T19:04:25Z (GMT). No. of bitstreams: 1 S0036-46651995000100007.pdf: 897597 bytes, checksum: 5cd06ce16898f7704f543141f811f999 (MD5) Previous issue date: 1995-02-01; Made availabl...

Date: 2014   |   Origin: Oasisbr

Detecção de DNA de Bartonella henselae em amostras clínicas, incluindo sangue p...

Kawasato, Karina Hatamoto; Oliveira, Léa Campos de; Velho, Paulo Eduardo Neves Ferreira; Yamamoto, Lidia; Del Negro, Gilda Maria Barbaro

Bacteria of the genus Bartonella are emerging pathogens detected in lymph node biopsies and aspirates probably caused by increased concentration of bacteria. Twenty-three samples of 18 patients with clinical, laboratory and/or epidemiological data suggesting bartonellosis were subjected to three nested amplifications targeting a fragment of the 60-kDa heat shock protein (HSP), the internal transcribed spacer 16...

Date: 2013   |   Origin: Oasisbr

Viability and molecular authentication of Coccidioides spp. isolates from the I...

Cavalcanti, Sarah Desirée Barbosa; Vidal, Mônica Scarpelli Martinelli; Sousa, Maria da Glória Teixeira de; Del Negro, Gilda Maria Barbaro

Coccidioidomycosis is an emerging fungal disease in Brazil; adequate maintenance and authentication of Coccidioides isolates are essential for research into genetic diversity of the environmental organisms, as well as for understanding the human disease. Seventeen Coccidioides isolates maintained under mineral oil since 1975 in the Instituto de Medicina Tropical de São Paulo (IMTSP) culture collection, Brazil, ...

Date: 2013   |   Origin: Oasisbr

Factors associated with hyperglycemia and low insulin levels in children underg...

Arkader, Ronaldo; Malbouisson, Luiz Marcelo; Del Negro, Gilda Maria Barbaro; Yamamoto, Lidia; Okay, Thelma Suely

OBJECTIVES: Administering steroids before cardiopulmonary bypass in pediatric heart surgery modulates systemic inflammatory response syndrome and improves postoperative recovery. However, the use of steroids aggravates hyperglycemia, which is associated with a poor prognosis. Adult patients with systemic inflammatory response syndrome usually evolve with hyperglycemia and high insulin levels, whereas >;90% of p...

Date: 2013   |   Origin: Oasisbr

First report of a clinical isolate of Candida haemulonii in Brazil

Del Negro, Gilda Maria Barbaro

Date: 2012   |   Origin: Oasisbr

First report of a clinical isolate of Candida haemulonii in Brazil

Almeida Jr., João Nobrega de; Motta, Adriana Lopes; Rossi, Flávia; Abdala, Edson; Pierrotti, Ligia Camera; Kono, Adriana Satie Gonçalves

Date: 2012   |   Origin: Oasisbr


Significant performance variation among PCR systems in diagnosing congenital to...

Okay, Thelma Suely; Yamamoto, Lidia; Oliveira, Léa Campos; Manuli, Erika Regina; Andrade Junior, Heitor Franco de; Del Negro, Gilda Maria Barbaro

INTRODUCTION: Performance variation among PCR systems in detecting Toxoplasma gondii has been extensively reported and associated with target genes, primer composition, amplification parameters, treatment during pregnancy, host genetic susceptibility and genotypes of different parasites according to geographical characteristics. PATIENTS: A total of 467 amniotic fluid samples from T. gondii IgM- and IgG-positiv...

Date: 2009   |   Origin: Oasisbr

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