5 documents found, page 1 of 1
Purification and primary structure determination of a galactose-specific lectin...
Silva, Helton Colares; Nagano, C. S.; Souza, Luis A.G.; Nascimento, K. S.; Isídro, Renato; Delatorre, Plínio; Rocha, Bruno Anderson Matias daVatairea guianensis seeds, a typical plant from the Brazilian Amazon region that belongs to the Dalbergieae tribe, possess a lectin that was isolated by precipitation with solid ammonium sulfate followed by guar gum affinity chromatography. This lectin was named VGL. The V. guianensis lectin strongly agglutinated rabbit erythrocytes and was inhibited by d-galactose and d-galactose-derived sugars, especially N-a...
A novel vasorelaxant lectin purified from seeds of Clathrotropis nitida: Partia...
Alves, Ana Cecília; Vasconcelos, Mayron Alves de; Santiago, Mayara Quiroz; Pinto-Junior, Vanir Reis; Osterne, Vinicius José SilvaA novel lectin from seeds of Clathrotropis nitida (CNA) was purified and characterized. CNA is a glycoprotein containing approximately 3.3% carbohydrates in its structure. CNA promoted intense agglutination of rabbit erythrocytes, which was inhibited by galactosides and porcine stomach mucin (PSM). The lectin maintained its hemagglutinating activity after incubation in a wide range of temperatures (30-60 °C) an...
Structural basis for both pro- and anti-inflammatory response induced by mannos...
Rocha, Bruno Anderson Matias da; Delatorre, Plínio; Oliveira, Taianá Maia; Benevides, R. G.; Pires, Alana de Freitas; Sousa, Albertina A.S.Legume lectins, despite high sequence homology, express diverse biological activities that vary in potency and efficacy. In studies reported here, the mannose-specific lectin from Cymbosema roseum (CRLI), which binds N-glycoproteins, shows both pro-inflammatory effects when administered by local injection and anti-inflammatory effects when by systemic injection. Protein sequencing was obtained by Tandem Mass Sp...
cDNA cloning and 1.75 Å crystal structure determination of PPL2, an endochitina...
Cavada, Benildo S.; Moreno, Frederico B. B. [UNESP]; Da Rocha, Bruno A. M.; De Azevedo Jr., Walter F.; Castellón, Rolando E. R.; Goersch, Georg V.Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:21:57Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:34:48Z : No. of bitstreams: 1 2-s2.0-33747413686.pdf: 511841 bytes, checksum: 1e5bb0ef6e0943c719824a16bc330739 (MD5); Made available in DSpace on 2014-05-27T11:21:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2006-09-01; Parkia platycephala lectin 2 was pu...
Purification of a PHA-like chitin-binding protein from Acacia farnesiana seeds ...
Santi-Gadelha, Tatiane; Rocha, Bruno Anderson Matias; Oliveira, Cecília Carvalho; Aragão, Karoline Sabóia; Marinho, EEmmanuel SilvaA lectin-like protein from the seeds of Acacia farnesiana was isolated from the albumin fraction, characterized, and sequenced by tandem mass spectrometry. The albumin fraction was extracted with 0.5 M NaCl, and the lectin-like protein of A. farnesiana (AFAL) was purified by ion-exchange chromatography (Mono-Q) followed by chromatofocusing. AFAL agglutinated rabbit erythrocytes and did not agglutinate human ABO...