7 documents found, page 1 of 1
Tubulin acetylation and the cellular mechanosensing and stress response
Carmona, Bruno; Delgado, Inês L. S.; Nolasco, Sofia; Marques, Rita; Gonçalves, João; Soares, HelenaMicrotubule (MT) acetylation has emerged as a critical regulator of cellular stress responses, integrating mechanical and oxidative stimuli to support cellular adaptability and survival. This post-translational modification (PTM) enhances MT flexibility and resilience, enabling cells to withstand mechanical challenges such as changes in extracellular matrix stiffness and applied forces. Through its impact on MT...
Combined TLR2/TLR4 activation equip non-mucosal dendritic cells to prime Th1 ce...
Zúquete, Sara; Ferreira, Mariana; Delgado, Inês L. S.; Gazalle, Paula; Andaluz, Stephanie; Rosa, Maria Teresa; Mendes, Ana Catarina; Santos, DulceActivated CD4+ T cells located at mucosal surfaces orchestrate local effector immune mechanisms. When properly polarized, these cells contribute to block infections at early stages and may be essential to restrain the local growth of mucosal tumors, playing a critical role in host protection. How CD4+ T cells simultaneously integrate gut-homing instructions and Th polarization signals transmitted by TLR-activat...
The apicomplexan parasite Toxoplasma gondii
Delgado, Inês L. S.; Zúquete, Sara; Santos, Dulce; Basto, Afonso P.; Leitão, Alexandre; Nolasco, SofiaToxoplasma gondii is a ubiquitous zoonotic parasite with an obligatory intracellular lifestyle. It relies on a specialized set of cytoskeletal and secretory organelles for host cell invasion. When infecting its felid definitive host, T. gondii undergoes sexual reproduction in the intestinal epithelium, producing oocysts that are excreted with the feces and sporulate in the environment. In other hosts and/or tis...
Impact of endemic Besnoitiosis on the performance of a dairy cattle herd
Anastácio, Catarina; Bexiga, Ricardo; Nolasco, Sofia; Zúquete, Sara; Delgado, Inês L. S.; Nunes, Telmo; Leitão, AlexandreThis study aimed to assess the effect of Besnoitia besnoiti infection on the reproductive and productive performance of a dairy cattle herd. A serological screening was performed by indirect fluorescent antibody test (IFAT) on every animal aged over one year (n = 262). Subsequently, 211 animals were clinically examined, with 96 of those being screened for detection of sclerocysts. The overall seroprevalence was...
Characterization of a MOB1 homolog in the Apicomplexan parasite toxoplasma gondii
Delgado, Inês L. S.; Tavares, Alexandra; Francisco, Samuel; Santos, Dulce; Coelho, João; Basto, Afonso P.; Zúquete, Sara; Müller, JoachimMonopolar spindle One Binder1 (MOB1) proteins are conserved components of the tumor-suppressing Hippo pathway, regulating cellular processes such as cytokinesis. Apicomplexan parasites present a life cycle that relies on the parasites’ ability to differentiate between stages and regulate their proliferation; thus, Hippo signaling pathways could play an important role in the regulation of the apicomplexan life c...
MOB: pivotal conserved proteins in cytokinesis, cell architecture and tissue ho...
Delgado, Inês L. S.; Carmona, Bruno; Nolasco, Sofia; Santos, Dulce; Leitão, Alexandre; Soares, HelenaThe MOB family proteins are constituted by highly conserved eukaryote kinase signal adaptors that are often essential both for cell and organism survival. Historically, MOB family proteins have been described as kinase activators participating in Hippo and Mitotic Exit Network/ Septation Initiation Network (MEN/SIN) signaling pathways that have central roles in regulating cytokinesis, cell polarity, cell prolif...
Mechanisms of transepithelial ammonia excretion and luminal alkalinization in t...
Wilson, Jonathan M.; Silva, Joana M.; Delgado, Inês L. S.; Ebanks, Sue C.; Vijayan, Mathilakath M.; Coimbra, J.; Grosell, MartinThe weatherloach, Misgurnus angulliacaudatus, is an intestinal air-breathing, freshwater fish that has the unique ability to excrete ammonia through gut volatilization when branchial and cutaneous routes are compromised during high environmental ammonia or air exposure. We hypothesized that transepithelial gut NH4 + transport is facilitated by an apical Na+/H+ (NH4 +) exchanger (NHE) and a basolateral Na+/K+(NH...