16 documents found, page 1 of 2
Molecular reprogramming in grapevine woody tissues at bud burst
Noronha, Henrique Luís Silva; Garcia, Virginie; Silva, Angélica; Delrot, Serge; Gallusci, Philippe; Gerós, H.Perennial woody plants undergo a period of dormancy from the beginning of autumn until the end of spring. Whereas the molecular and physiological events that characterize dormancy release of buds have been described in detail, those occurring in woody tissues underneath the buds are mostly unknown. To bridge this gap, the mRNA populations of cane segments located underneath the bud were analyzed at bud dormancy...
Correction to: A molecular perspective on starch metabolism in woody tissues
Noronha, Henrique Luís Silva; Silva, Angélica; Dai, Zhanwu; Gallusci, Philippe; Rombolà, Adamo D.; Delrot, Serge; Gerós, H.The original article was corrected.
A molecular perspective on starch metabolism in woody tissues
Noronha, Henrique; Silva, Angélica; Dai, Zhanwu; Gallusci, Philippe; Rombolà, Adamo D.; Delrot, Serge; Gerós, H.The elucidation of the molecular mechanisms of starch synthesis and mobilization in perennial woody tissues is of the utmost scientific and agricultural importance. Starch is the main carbohydrate reserve in plants and is fundamental in human nutrition and several industrial processes. In leaves, starch accumulated during the day is degraded throughout the night and the resulting sugars, glucose and maltose, ar...
Low source-sink ratio reduces reserve starch in grapevine woody canes and modul...
Silva, Angélica; Noronha, Henrique; Dai, Zhanwu; Delrot, Serge; Gerós, H.Severe leaf removal decreases storage starch and sucrose in grapevine cv. Cabernet Sauvignon fruiting cuttings and modulates the activity of key enzymes and the expression of sugar transporter genes.
The grapevine uncharacterized intrinsic protein 1 (VvXIP1) is regulated by drou...
Noronha, Henrique; Araujo, Diogo; Conde, Carlos; Martins, Ana P.; Soveral, Graca; Chaumont, Francois; Delrot, Serge; Gerós, H.A MIP (Major Intrinsic Protein) subfamily called Uncharacterized Intrinsic Proteins (XIP) was recently described in several fungi and eudicot plants. In this work, we cloned a XIP from grapevine, VvXIP1, and agrobacterium-mediated transformation studies in Nicotiana benthamiana revealed that the encoded aquaporin shows a preferential localization at the endoplasmic reticulum membrane. Stopped-flow spectrometry ...
Grape berry vacuole: a complex and heterogeneous membrane system specialized in...
Fontes, N.; Gerós, H.; Delrot, SergeVacuoles fulfill highly specialized functions depending on cell type and tissue and plant developmental stage. This complex and dynamic organelle is the main reservoir of grape berry cells, playing a major role during fruit development and ripening. Berry development is accompanied by modifications in size, composition, color, texture, flavor, and pathogen susceptibility, primarily because of changes in vacuola...
Biochemical changes throughout grape berry development and fruit and wine quality
Conde, C.; Silva, Paulo; Fontes, N.; Dias, Alberto Carlos Pires; Tavares, R. M.; Sousa, Maria João; Agasse, A.; Delrot, Serge; Gerós, H.Wine is made up of more than one thousand compounds, the majority of which, such as vitamins and minerals, come from the grapes, while others, like ethanol and glycerol, are products of the winemaking process. Although sugars are either partially or completely transformed, sugar import and accumulation into the ripening berry is a major parameter of wine quality. Sugar status is directly related to the final al...
Utilization and transport of mannitol in Olea europaea and implications on salt...
Silva, Paulo; Conde, C.; Agasse, A.; Lemoine, Remi; Delrot, Serge; Tavares, R. M.; Gerós, H.Mannitol is one of the primary photosynthetic products and major phloem-translocated carbohydrate in Olea europaea L., an important and widespread crop in the Mediterranean basin. In O. europaea cell suspensions, we have previously identified and characterized a H+-dependent polyol transport system whose activity was correlated with OeMaT1 (Olea europaea Mannitol Transporter) expression. Addition of 100-500 mM ...
Pathways of glucose regulation of monosaccharide transport in grape cells
Conde, Carlos; Agasse, A.; Glissant, David; Tavares, R. M.; Gerós, H.; Delrot, SergeGrape (Vitis vinifera) heterotrophic suspension-cultured cells were used as a model system to study glucose (Glc) transport and its regulation. Cells transported D-[14C]Glc according to simple Michaelis-Menten kinetics superimposed on first-order kinetics. The saturating component is a high-affinity, broad-specificity H+-dependent transport system (Km = 0.05 mM). Glc concentration in the medium tightly regulate...
Mechanisms underlying the adjustment of cell volume of Olea europaea in respons...
Silva, Paulo; Conde, C.; Agasse, A.; Delrot, Serge; Sampaio, Paula; Tavares, R. M.; Gerós, H.Olea europaea cell cultures we have previously identified and characterized a polyol:H+ symport system whose activity was correlated with OeMaT1 (Olea europaea Mannitol Transporter) expression. Salt (100-500 mM NaCl) enhances the capacity of the transport system and the amount of OeMaT1 transcripts, whereas it strongly represses mannitol dehydrogenase activity, enzyme responsible for the first step of the polyo...